Rabbit Recombinant Monoclonal FGFR2 antibody. Suitable for WB, IHC-P and reacts with Transfected cell lysate - Human, Human samples.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
WB | IHC-P | |
---|---|---|
Human | Tested | Tested |
Transfected cell lysate - Human | Tested | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Transfected cell lysate - Human | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100 - 1/500 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Transfected cell lysate - Human | Dilution info - | Notes - |
Tyrosine-protein kinase that acts as a cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation, migration and apoptosis, and in the regulation of embryonic development. Required for normal embryonic patterning, trophoblast function, limb bud development, lung morphogenesis, osteogenesis and skin development. Plays an essential role in the regulation of osteoblast differentiation, proliferation and apoptosis, and is required for normal skeleton development. Promotes cell proliferation in keratinocytes and immature osteoblasts, but promotes apoptosis in differentiated osteoblasts. Phosphorylates PLCG1, FRS2 and PAK4. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. FGFR2 signaling is down-regulated by ubiquitination, internalization and degradation. Mutations that lead to constitutive kinase activation or impair normal FGFR2 maturation, internalization and degradation lead to aberrant signaling. Over-expressed FGFR2 promotes activation of STAT1.
BEK, KGFR, KSAM, Fibroblast growth factor receptor 2, FGFR-2, K-sam, Keratinocyte growth factor receptor, KGFR
Rabbit Recombinant Monoclonal FGFR2 antibody. Suitable for WB, IHC-P and reacts with Transfected cell lysate - Human, Human samples.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
EPR30797-567
Affinity purification Protein A
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling FGFR2IIIb/FGFR2b with ab322553 at 1/100 (5.0 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on human cerebrum. The section was incubated with ab322553 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemical analysis of paraffin-embedded (A) KATO III (human stomach spherical cell) cell pellet. (B) HepG2 (human hepatocellular carcinoma epithelial cell) cell pellet. tissue labeling FGFR2IIIb/FGFR2b with ab322553 at 1/500 (1.0 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on KATO III (image A). Negative staining on HepG2 (image B). The section was incubated with ab322553 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemical analysis of paraffin-embedded Human endometrial carcinoma tissue labeling FGFR2IIIb/FGFR2b with ab322553 at 1/100 (5.0 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human endometrial carcinoma. The section was incubated with ab322553 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling FGFR2IIIb/FGFR2b with ab322553 at 1/100 (5.0 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human ovarian carcinoma. The section was incubated with ab322553 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemical analysis of paraffin-embedded Human stomach adenocarcinoma tissue labeling FGFR2IIIb/FGFR2b with ab322553 at 1/100 (5.0 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human stomach adenocarcinoma (only one of 45 human stomach adenocarcinoma cases was positive). The section was incubated with ab322553 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This antibody does not cross-react with human FGFR2IIIc, FGFR3IIIb and FGFR1IIIb.
Samples are non-boiled as boiling may cause protein aggregation.
In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
All lanes: Western blot - Anti-FGFR2IIIb/FGFR2b antibody [EPR30797-567] (ab322553) at 1/1000 dilution
Lane 1: 293T cells transfected with a human FGFR2IIIb expression vector containing a His-tag, whole cell lysate at 20 µg
Lane 2: 293T cells transfected with a human FGFR2IIIc expression vector containing a His-tag, whole cell lysate at 20 µg
Lane 3: 293T cells transfected with a human FGFR3IIIb expression vector containing a His-tag, whole cell lysate at 20 µg
Lane 4: 293T cells transfected with a human FGFR1IIIb expression vector containing a His-tag, whole cell lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 135 kDa
Exposure time: 3s
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: HepG2(PMID: 20093481)
Low expression: LoVo(PMID: PMID:22701813)
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 24968263).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-FGFR2IIIb/FGFR2b antibody [EPR30797-567] (ab322553) at 1/1000 dilution
Lane 1: KATO III (human stomach spherical) whole cell lysate at 40 µg
Lane 2: HepG2 (human hepatocelullar carcinoma epithelial cell) whole cell lysate at 40 µg
Lane 3: LoVo (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 40 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 85-135 kDa, 36 kDa
Exposure time: 3s
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