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Rabbit Recombinant Monoclonal FGFR2 antibody. Suitable for WB, IHC-P and reacts with Transfected cell lysate - Human, Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGFR2IIIb/FGFR2b antibody [EPR30797-567] (AB322553), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGFR2IIIb/FGFR2b antibody [EPR30797-567] (AB322553), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGFR2IIIb/FGFR2b antibody [EPR30797-567] (AB322553), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGFR2IIIb/FGFR2b antibody [EPR30797-567] (AB322553), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGFR2IIIb/FGFR2b antibody [EPR30797-567] (AB322553), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Tested
Transfected cell lysate - Human
Tested
Not recommended

Tested
Tested

Species

Transfected cell lysate - Human

Dilution info

1/1000

Notes

-

Species

Human

Dilution info

1/1000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100 - 1/500

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Transfected cell lysate - Human

Dilution info

-

Notes

-

Target data

Function

Tyrosine-protein kinase that acts as a cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation, migration and apoptosis, and in the regulation of embryonic development. Required for normal embryonic patterning, trophoblast function, limb bud development, lung morphogenesis, osteogenesis and skin development. Plays an essential role in the regulation of osteoblast differentiation, proliferation and apoptosis, and is required for normal skeleton development. Promotes cell proliferation in keratinocytes and immature osteoblasts, but promotes apoptosis in differentiated osteoblasts. Phosphorylates PLCG1, FRS2 and PAK4. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. FGFR2 signaling is down-regulated by ubiquitination, internalization and degradation. Mutations that lead to constitutive kinase activation or impair normal FGFR2 maturation, internalization and degradation lead to aberrant signaling. Over-expressed FGFR2 promotes activation of STAT1.

Alternative names

Rabbit Recombinant Monoclonal FGFR2 antibody. Suitable for WB, IHC-P and reacts with Transfected cell lysate - Human, Human samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR30797-567

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGFR2IIIb/FGFR2b antibody [EPR30797-567] (ab322553), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGFR2IIIb/FGFR2b antibody [EPR30797-567] (ab322553)

    Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling FGFR2IIIb/FGFR2b with ab322553 at 1/100 (5.0 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: no staining on human cerebrum. The section was incubated with ab322553 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGFR2IIIb/FGFR2b antibody [EPR30797-567] (ab322553), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGFR2IIIb/FGFR2b antibody [EPR30797-567] (ab322553)

    Immunohistochemical analysis of paraffin-embedded (A) KATO III (human stomach spherical cell) cell pellet. (B) HepG2 (human hepatocellular carcinoma epithelial cell) cell pellet. tissue labeling FGFR2IIIb/FGFR2b with ab322553 at 1/500 (1.0 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on KATO III (image A). Negative staining on HepG2 (image B). The section was incubated with ab322553 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGFR2IIIb/FGFR2b antibody [EPR30797-567] (ab322553), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGFR2IIIb/FGFR2b antibody [EPR30797-567] (ab322553)

    Immunohistochemical analysis of paraffin-embedded Human endometrial carcinoma tissue labeling FGFR2IIIb/FGFR2b with ab322553 at 1/100 (5.0 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human endometrial carcinoma. The section was incubated with ab322553 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGFR2IIIb/FGFR2b antibody [EPR30797-567] (ab322553), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGFR2IIIb/FGFR2b antibody [EPR30797-567] (ab322553)

    Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling FGFR2IIIb/FGFR2b with ab322553 at 1/100 (5.0 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human ovarian carcinoma. The section was incubated with ab322553 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGFR2IIIb/FGFR2b antibody [EPR30797-567] (ab322553), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGFR2IIIb/FGFR2b antibody [EPR30797-567] (ab322553)

    Immunohistochemical analysis of paraffin-embedded Human stomach adenocarcinoma tissue labeling FGFR2IIIb/FGFR2b with ab322553 at 1/100 (5.0 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human stomach adenocarcinoma (only one of 45 human stomach adenocarcinoma cases was positive). The section was incubated with ab322553 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Western blot - Anti-FGFR2IIIb/FGFR2b antibody [EPR30797-567] (ab322553), expandable thumbnail

    Western blot - Anti-FGFR2IIIb/FGFR2b antibody [EPR30797-567] (ab322553)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This antibody does not cross-react with human FGFR2IIIc, FGFR3IIIb and FGFR1IIIb.

    Samples are non-boiled as boiling may cause protein aggregation.

    In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.

    All lanes: Western blot - Anti-FGFR2IIIb/FGFR2b antibody [EPR30797-567] (ab322553) at 1/1000 dilution

    Lane 1: 293T cells transfected with a human FGFR2IIIb expression vector containing a His-tag, whole cell lysate at 20 µg

    Lane 2: 293T cells transfected with a human FGFR2IIIc expression vector containing a His-tag, whole cell lysate at 20 µg

    Lane 3: 293T cells transfected with a human FGFR3IIIb expression vector containing a His-tag, whole cell lysate at 20 µg

    Lane 4: 293T cells transfected with a human FGFR1IIIb expression vector containing a His-tag, whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 135 kDa

    Exposure time: 3s

  • Western blot - Anti-FGFR2IIIb/FGFR2b antibody [EPR30797-567] (ab322553), expandable thumbnail

    Western blot - Anti-FGFR2IIIb/FGFR2b antibody [EPR30797-567] (ab322553)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: HepG2(PMID: 20093481)

    Low expression: LoVo(PMID: PMID:22701813)

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 24968263).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-FGFR2IIIb/FGFR2b antibody [EPR30797-567] (ab322553) at 1/1000 dilution

    Lane 1: KATO III (human stomach spherical) whole cell lysate at 40 µg

    Lane 2: HepG2 (human hepatocelullar carcinoma epithelial cell) whole cell lysate at 40 µg

    Lane 3: LoVo (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 40 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 85-135 kDa, 36 kDa

    Exposure time: 3s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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