Rabbit Monoclonal FGFR3 antibody. Carrier free. Suitable for WB and reacts with Dog, Cat samples. Immunogen corresponding to Synthetic Peptide within Human FGFR3.
Images
![Western blot - Anti-FGFR3 antibody [HL1595] (AB308173), expandable thumbnail](https://content.abcam.com/products/images/fgfr3-antibody-hl1595-ab308173--western-blot-img407078.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-FGFR3 antibody [HL1595] (AB308173)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- Synthetic Peptide within Human FGFR3. The exact immunogen used to generate this antibody is proprietary information. Database link P22607
Reactivity data
Select an application| WB | |
|---|---|
| Cat | Tested |
| Dog | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Dog | Dilution info 1/500 | Notes - |
Species Cat | Dilution info 1/500 | Notes - |
Target data
Function
Tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation and apoptosis. Plays an essential role in the regulation of chondrocyte differentiation, proliferation and apoptosis, and is required for normal skeleton development. Regulates both osteogenesis and postnatal bone mineralization by osteoblasts. Promotes apoptosis in chondrocytes, but can also promote cancer cell proliferation. Required for normal development of the inner ear. Phosphorylates PLCG1, CBL and FRS2. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Plays a role in the regulation of vitamin D metabolism. Mutations that lead to constitutive kinase activation or impair normal FGFR3 maturation, internalization and degradation lead to aberrant signaling. Over-expressed or constitutively activated FGFR3 promotes activation of PTPN11/SHP2, STAT1, STAT5A and STAT5B. Secreted isoform 3 retains its capacity to bind FGF1 and FGF2 and hence may interfere with FGF signaling.
Alternative names
Fibroblast growth factor receptor 3, FGFR-3, FGFR3, JTK4
Recommended products
Rabbit Monoclonal FGFR3 antibody. Carrier free. Suitable for WB and reacts with Dog, Cat samples. Immunogen corresponding to Synthetic Peptide within Human FGFR3.
Alternative names
Fibroblast growth factor receptor 3, FGFR-3, FGFR3, JTK4
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- Synthetic Peptide within Human FGFR3. The exact immunogen used to generate this antibody is proprietary information. Database link P22607
- Carrier free
Yes
- Clone number
HL1595
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Supplementary info
- Activity summary
FGFR3 or fibroblast growth factor receptor 3 is a tyrosine kinase receptor with a molecular mass of approximately 95 kDa. This receptor also known as CD333 primarily interacts with fibroblast growth factors. It resides on the cell surface and is predominately expressed in bone cartilage and the brain. FGFR3 plays a major role in cell growth regulation and differentiation affecting how cells respond to external signals.
- Biological function summary
FGFR3 participates in important processes such as chondrogenesis and osteogenesis which are essential for bone growth and development. The receptor operates within a complex system involving interactions with ligand-induced dimerization and autophosphorylation. This phosphorylation event activates the receptor's intrinsic kinase activity further triggering downstream signaling pathways for cellular responses.
- Pathways
FGFR3 is involved with the MAPK/ERK and PI3K/AKT pathways which play significant roles in cell proliferation differentiation and survival. FGFR3 links closely with FGF ligands and similar receptor types such as FGFR1 and FGFR2 within these pathways. Differences in pathway activation can lead to diverse biological responses illustrating its pivotal role in maintaining cellular homeostasis.
- Associated diseases and disorders
FGFR3 mutations have significant links to skeletal disorders most notably achondroplasia characterized by short limbs and abnormal bone growth. FGFR3 also associates with bladder cancer where overactivity of the receptor leads to increased cell proliferation and survival. Mutations in FGFR3 often correlate with these conditions impacting signaling pathways and interacting with proteins such as PTPN11 highlighting its critical role in pathogenesis.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Western blot - Anti-FGFR3 antibody [HL1595] (ab308173), expandable thumbnail](https://content.abcam.com/products/images/fgfr3-antibody-hl1595-ab308173--western-blot-img407078.jpg "Western blot - Anti-FGFR3 antibody [HL1595] (ab308173)")
Western blot - Anti-FGFR3 antibody [HL1595] (ab308173)
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FGFR3 antibody [HL1595] (ab308173) diluted at 1/500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
All lanes: Western blot - Anti-FGFR3 antibody [HL1595] (ab308173) at 1/500 dilution
Lane 1: MDCK cell lysate at 30 µg
Lane 2: PG-4 cell lysate at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 87.7 kDa
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