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AB155960

Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)]

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(1 Review)

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(13 Publications)

Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (ab155960) is a rabbit monoclonal antibody detecting FGFR3 in Western Blot, Flow Cytometry (Intra), IP, ICC/IF, Dot Blot. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency
- Over 10 publications

View Alternative Names

CD333, JTK4, FGFR3, Fibroblast growth factor receptor 3, FGFR-3

7 Images
Immunocytochemistry/ Immunofluorescence - Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (AB155960)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (AB155960)

Immunocytochemistry/Immunofluorescence analysis of untreated, Per treated and Per + LP treated MCF7 cells labelling FGFR3 (phospho Y724) with ab155960 (left) and FGFR3 with ab137084 (right) both at a dilution of 1/200.

Cells were fixed with 100% methanol. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

The image shows increased cytoplasmic staining after Pervanadate (1 mM, 30 min) treatment on MCF7 cells. The LP treatment decreased the cytoplasmic staining caused by Pervanadate.

ab137084 was used as a Pan control for ab155960. The results showed cytoplasmic staining on untreated, Per treated and Per + LP treated MCF7 cells.

Flow Cytometry (Intracellular) - Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (AB155960)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (AB155960)

Intracellular Flow Cytometry analysis of MCF7 (human breast carcinoma) treated with 1 mM pervanadate for 30 minutes cells labeling FGFR3 (phospho Y724) with purified ab155960 at 1/50 dilution (red). The secondary antibody was Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution. Green shows untreated MCF7 (human breast carcinoma) cells. A Rabbit monoclonal IgG (Black) was used as the isotype control and cells without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.

Immunoprecipitation - Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (AB155960)
  • IP

Lab

Immunoprecipitation - Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (AB155960)

ab155960 (unpurified) at 1/15 immunoprecipitating FGFR3 (phospho Y724) in MCF7 cells treated with pervanadate. For western blotting, a peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1000).

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Immunoprecipitation - Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (ab155960)

Predicted band size: 88 kDa

Observed band size: 98 kDa

false

Immunoprecipitation - Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (AB155960)
  • IP

Lab

Immunoprecipitation - Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (AB155960)

ab155960 (purified) at 1/50 immunoprecipitating FGFR3 (phospho Y724) in MCF7 cells treated with pervanadate. For western blotting, a peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1000).

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Immunoprecipitation - Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (ab155960)

Predicted band size: 88 kDa

Observed band size: 98 kDa

false

Western blot - Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (AB155960)
  • WB

Lab

Western blot - Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (AB155960)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (ab155960) at 1/10000 dilution

Lane 1:

MCF7 whole cell lysate - treated with pervanadate at 10 µg

Lane 2:

MCF7 whole cell lysate - untreated at 10 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 88 kDa

Observed band size: 98 kDa

false

Exposure time: 30s

Western blot - Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (AB155960)
  • WB

Lab

Western blot - Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (AB155960)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (ab155960) at 1/20000 dilution

Lane 1:

MCF7 cell lysate - untreated at 10 µg

Lane 2:

MCF7 cell lysate - treated with pervanadate at 10 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 88 kDa

Observed band size: 96 kDa

false

Dot Blot - Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (AB155960)
  • Dot

Lab

Dot Blot - Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (AB155960)

Dot blot analysis of human FGFR3 (pY724) phospho peptide (lane 1) and human FGFR3 non-phospho peptide (lane 2) labelling FGFR2 (phospho Y724) with ab155960 at a dilution of 1/1000. A peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/2500).

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 10 seconds.

  • Carrier free

    Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] - BSA and Azide free

  • Carrier free

    Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] - Low endotoxin, Azide free

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)]

  • 660 APC

    APC Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2281(3)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra), ICC/IF, WB, IP, Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

What is this antibody validated in?
Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (ab155960) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunocytochemistry/immunofluorescence (ICC/IF), Dot Blot in Human samples.

What is the molecular weight of FGFR3?
Anti-FGFR3 (phospho Y724) [EPR2281(3)] (ab155960) specifically detects a band for FGFR3 (UniProt: P22607) at a molecular weight of 88kDa.

Trusted by the scientific community
Anti-FGFR3 (phospho Y724) [EPR2281(3)] (ab155960) was first used in a scientific publication in 2013 and has been cited over 10 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR2281(3)] also available for your convenience: ab155960, Carrier free - ab215385, Alexa Fluor® 488 - ab237164, Alexa Fluor® 647 - ab237165, Carrier free - ab271918, APC - ab303576

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FGFR3 or fibroblast growth factor receptor 3 is a tyrosine kinase receptor with a molecular mass of approximately 95 kDa. This receptor also known as CD333 primarily interacts with fibroblast growth factors. It resides on the cell surface and is predominately expressed in bone cartilage and the brain. FGFR3 plays a major role in cell growth regulation and differentiation affecting how cells respond to external signals.
Biological function summary

FGFR3 participates in important processes such as chondrogenesis and osteogenesis which are essential for bone growth and development. The receptor operates within a complex system involving interactions with ligand-induced dimerization and autophosphorylation. This phosphorylation event activates the receptor's intrinsic kinase activity further triggering downstream signaling pathways for cellular responses.

Pathways

FGFR3 is involved with the MAPK/ERK and PI3K/AKT pathways which play significant roles in cell proliferation differentiation and survival. FGFR3 links closely with FGF ligands and similar receptor types such as FGFR1 and FGFR2 within these pathways. Differences in pathway activation can lead to diverse biological responses illustrating its pivotal role in maintaining cellular homeostasis.

FGFR3 mutations have significant links to skeletal disorders most notably achondroplasia characterized by short limbs and abnormal bone growth. FGFR3 also associates with bladder cancer where overactivity of the receptor leads to increased cell proliferation and survival. Mutations in FGFR3 often correlate with these conditions impacting signaling pathways and interacting with proteins such as PTPN11 highlighting its critical role in pathogenesis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Tyrosine-protein kinase that acts as a cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation and apoptosis. Plays an essential role in the regulation of chondrocyte differentiation, proliferation and apoptosis, and is required for normal skeleton development. Regulates both osteogenesis and postnatal bone mineralization by osteoblasts. Promotes apoptosis in chondrocytes, but can also promote cancer cell proliferation. Required for normal development of the inner ear. Phosphorylates PLCG1, CBL and FRS2. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Plays a role in the regulation of vitamin D metabolism. Mutations that lead to constitutive kinase activation or impair normal FGFR3 maturation, internalization and degradation lead to aberrant signaling. Over-expressed or constitutively activated FGFR3 promotes activation of PTPN11/SHP2, STAT1, STAT5A and STAT5B. Secreted isoform 3 retains its capacity to bind FGF1 and FGF2 and hence may interfere with FGF signaling.
See full target information FGFR3 phospho Y724
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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Genes & diseases 11:479-494 PubMed37588207

2023

A novel FGFR1 inhibitor CYY292 suppresses tumor progression, invasion, and metastasis of glioblastoma by inhibiting the Akt/GSK3β/snail signaling axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yanran Bi,Ruiling Zheng,Jiahao Hu,Ruiqing Shi,Junfeng Shi,Yutao Wang,Peng Wang,Wenyi Jiang,Gyudong Kim,Zhiguo Liu,Xiaokun Li,Li Lin

Cancer cell international 22:295 PubMed36167542

2022

METTL3 suppresses anlotinib sensitivity by regulating mA modification of FGFR3 in oral squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Chen,Shuai Li,Zhexun Huang,Congyuan Cao,Anxun Wang,Qianting He

Cell reports 39:110993 PubMed35732135

2022

KRAS-independent feedback activation of wild-type RAS constrains KRAS inhibitor efficacy.

Applications

Unspecified application

Species

Unspecified reactive species

Meagan B Ryan,Oluwadara Coker,Alexey Sorokin,Katerina Fella,Haley Barnes,Edmond Wong,Preeti Kanikarla,Fengqin Gao,Youyan Zhang,Lian Zhou,Scott Kopetz,Ryan B Corcoran

Scientific reports 11:11005 PubMed34040128

2021

Gait disturbances and muscle dysfunction in fibroblast growth factor 2 knockout mice.

Applications

Unspecified application

Species

Unspecified reactive species

C Homer-Bouthiette,L Xiao,Marja M Hurley

Cancer management and research 12:6767-6777 PubMed32801905

2020

Estrogen-Induced Stromal FGF18 Promotes Proliferation and Invasion of Endometrial Carcinoma Cells Through ERK and Akt Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Wu,Xiang Tao,Hong Zhang,Xiang-Hua Yi,Yin-Hua Yu

Journal of experimental & clinical cancer research 38:481 PubMed31801598

2019

Shisa3 brakes resistance to EGFR-TKIs in lung adenocarcinoma by suppressing cancer stem cell properties.

Applications

Unspecified application

Species

Unspecified reactive species

Jiahui Si,Yuanyuan Ma,Ji Wang Bi,Ying Xiong,Chao Lv,Shaolei Li,Nan Wu,Yue Yang

Brain research bulletin 152:285-296 PubMed31220553

2019

FGF9 alters the Wallerian degeneration process by inhibiting Schwann cell transformation and accelerating macrophage infiltration.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjing Lv,Binbin Deng,Weisong Duan,Yi Li,Xueqin Song,Yingxiao Ji,Zhongyao Li,Yakun Liu,Xiaoxiao Wang,Chunyan Li

Biochemical and biophysical research communication 514:618-624 PubMed31076107

2019

Mg-Zn-Mn alloy extract induces the angiogenesis of human umbilical vein endothelial cells via FGF/FGFR signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ding Li,Qi Yuan,Kun Yu,Tao Xiao,Lihong Liu,Yilong Dai,Liang Xiong,Boyu Zhang,Aoyu Li

Nature communications 8:1339 PubMed29109536

2017

Self-patterning of rostral-caudal neuroectoderm requires dual role of Fgf signaling for localized Wnt antagonism.

Applications

Unspecified application

Species

Unspecified reactive species

Nozomu Takata,Eriko Sakakura,Mototsugu Eiraku,Takeya Kasukawa,Yoshiki Sasai

Nature communications 8:607 PubMed28928360

2017

Tumor-associated B-cells induce tumor heterogeneity and therapy resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Rajasekharan Somasundaram,Gao Zhang,Mizuho Fukunaga-Kalabis,Michela Perego,Clemens Krepler,Xiaowei Xu,Christine Wagner,Denitsa Hristova,Jie Zhang,Tian Tian,Zhi Wei,Qin Liu,Kanika Garg,Johannes Griss,Rufus Hards,Margarita Maurer,Christine Hafner,Marius Mayerhöfer,Georgios Karanikas,Ahmad Jalili,Verena Bauer-Pohl,Felix Weihsengruber,Klemens Rappersberger,Josef Koller,Roland Lang,Courtney Hudgens,Guo Chen,Michael Tetzlaff,Lawrence Wu,Dennie Tompers Frederick,Richard A Scolyer,Georgina V Long,Manashree Damle,Courtney Ellingsworth,Leon Grinman,Harry Choi,Brian J Gavin,Margaret Dunagin,Arjun Raj,Nathalie Scholler,Laura Gross,Marilda Beqiri,Keiryn Bennett,Ian Watson,Helmut Schaider,Michael A Davies,Jennifer Wargo,Brian J Czerniecki,Lynn Schuchter,Dorothee Herlyn,Keith Flaherty,Meenhard Herlyn,Stephan N Wagner
View all publications
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