Rabbit Recombinant Monoclonal FGFR3 phospho Y724 antibody. Suitable for IP, Dot, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 11 publications.
Images
![Immunoprecipitation - Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (AB155960), expandable thumbnail](https://content.abcam.com/products/images/fgfr3-phospho-y724-antibody-epr22813-ab155960--immunoprecipitation-img129595.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (AB155960)")
![Western blot - Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (AB155960), expandable thumbnail](https://content.abcam.com/products/images/fgfr3-phospho-y724-antibody-epr22813-ab155960--western-blot-img24643.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (AB155960)")
![Immunoprecipitation - Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (AB155960), expandable thumbnail](https://content.abcam.com/products/images/fgfr3-phospho-y724-antibody-epr22813-ab155960--immunoprecipitation-img110587.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (AB155960)")
![Flow Cytometry (Intracellular) - Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (AB155960), expandable thumbnail](https://content.abcam.com/products/images/fgfr3-phospho-y724-antibody-epr22813-ab155960--flow-cytometry-intracellular-img26290.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (AB155960)")
![Immunocytochemistry/ Immunofluorescence - Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (AB155960), expandable thumbnail](https://content.abcam.com/products/images/fgfr3-phospho-y724-antibody-epr22813-ab155960--immunocytochemistry-immunofluorescence-img121559.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (AB155960)")
Publications
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- Cell reports 39:1109932022KRAS-independent feedback activation of wild-type RAS constrains KRAS inhibitor efficacy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMeagan B Ryan,Oluwadara Coker,Alexey Sorokin,Katerina Fella,Haley Barnes,Edmond Wong,Preeti Kanikarla,Fengqin Gao,Youyan Zhang,Lian Zhou,Scott Kopetz,Ryan B CorcoranPubMed 35732135
- Scientific reports 11:110052021Gait disturbances and muscle dysfunction in fibroblast growth factor 2 knockout mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesC Homer-Bouthiette,L Xiao,Marja M HurleyPubMed 34040128
- Cancer management and research 12:6767-67772020Estrogen-Induced Stromal FGF18 Promotes Proliferation and Invasion of Endometrial Carcinoma Cells Through ERK and Akt Signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJian Wu et. al.PubMed 32801905
- Journal of experimental & clinical cancer research 38:4812019Shisa3 brakes resistance to EGFR-TKIs in lung adenocarcinoma by suppressing cancer stem cell properties.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiahui Si et. al.PubMed 31801598
- Brain research bulletin 152:285-2962019FGF9 alters the Wallerian degeneration process by inhibiting Schwann cell transformation and accelerating macrophage infiltration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenjing Lv et. al.PubMed 31220553
- Biochemical and biophysical research communication 514:618-6242019Mg-Zn-Mn alloy extract induces the angiogenesis of human umbilical vein endothelial cells via FGF/FGFR signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDing Li et. al.PubMed 31076107
- Nature communications 8:13392017Self-patterning of rostral-caudal neuroectoderm requires dual role of Fgf signaling for localized Wnt antagonism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNozomu Takata et. al.PubMed 29109536
- Nature communications 8:6072017Tumor-associated B-cells induce tumor heterogeneity and therapy resistance.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRajasekharan Somasundaram et. al.PubMed 28928360
- Molecular cancer therapeutics 16:614-6242017Akt Activation Mediates Acquired Resistance to Fibroblast Growth Factor Receptor Inhibitor BGJ398.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJharna Datta et. al.PubMed 28255027
- Clinical cancer research : an official journal of the American Association for Cancer Research 23:3003-30112016A Phase II Trial of Dovitinib in BCG-Unresponsive Urothelial Carcinoma with Mutations or Overexpression: Hoosier Cancer Research Network Trial HCRN 12-157.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNoah M Hahn,Trinity J Bivalacqua,Ashley E Ross,George J Netto,Alex Baras,Jong Chul Park,Carolyn Chapman,Timothy A Masterson,Michael O Koch,Richard Bihrle,Richard S Foster,Thomas A Gardner,Liang Cheng,David R Jones,Kyle McElyea,George E Sandusky,Timothy Breen,Ziyue Liu,Costantine Albany,Marietta L Moore,Rhoda L Loman,Angela Reed,Scott A Turner,Francine B De Abreu,Torrey Gallagher,Gregory J Tsongalis,Elizabeth R Plimack,Richard E Greenberg,Daniel M GeynismanPubMed 27932416
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Abcam Recommends
Reactivity data
Select an application| IP | Dot | WB | IHC-P | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Not recommended | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted | Not recommended | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/15 - 1/50 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/80 - 1/300 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
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1 product for Alternative Version
Target data
Function
Tyrosine-protein kinase that acts as a cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation and apoptosis. Plays an essential role in the regulation of chondrocyte differentiation, proliferation and apoptosis, and is required for normal skeleton development. Regulates both osteogenesis and postnatal bone mineralization by osteoblasts. Promotes apoptosis in chondrocytes, but can also promote cancer cell proliferation. Required for normal development of the inner ear. Phosphorylates PLCG1, CBL and FRS2. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Plays a role in the regulation of vitamin D metabolism. Mutations that lead to constitutive kinase activation or impair normal FGFR3 maturation, internalization and degradation lead to aberrant signaling. Over-expressed or constitutively activated FGFR3 promotes activation of PTPN11/SHP2, STAT1, STAT5A and STAT5B. Secreted isoform 3 retains its capacity to bind FGF1 and FGF2 and hence may interfere with FGF signaling.
Alternative names
CD333, JTK4, FGFR3, Fibroblast growth factor receptor 3, FGFR-3
Recommended products
Rabbit Recombinant Monoclonal FGFR3 phospho Y724 antibody. Suitable for IP, Dot, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 11 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR2281(3)
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
FGFR3 or fibroblast growth factor receptor 3 is a tyrosine kinase receptor with a molecular mass of approximately 95 kDa. This receptor also known as CD333 primarily interacts with fibroblast growth factors. It resides on the cell surface and is predominately expressed in bone cartilage and the brain. FGFR3 plays a major role in cell growth regulation and differentiation affecting how cells respond to external signals.
- Biological function summary
FGFR3 participates in important processes such as chondrogenesis and osteogenesis which are essential for bone growth and development. The receptor operates within a complex system involving interactions with ligand-induced dimerization and autophosphorylation. This phosphorylation event activates the receptor's intrinsic kinase activity further triggering downstream signaling pathways for cellular responses.
- Pathways
FGFR3 is involved with the MAPK/ERK and PI3K/AKT pathways which play significant roles in cell proliferation differentiation and survival. FGFR3 links closely with FGF ligands and similar receptor types such as FGFR1 and FGFR2 within these pathways. Differences in pathway activation can lead to diverse biological responses illustrating its pivotal role in maintaining cellular homeostasis.
- Associated diseases and disorders
FGFR3 mutations have significant links to skeletal disorders most notably achondroplasia characterized by short limbs and abnormal bone growth. FGFR3 also associates with bladder cancer where overactivity of the receptor leads to increased cell proliferation and survival. Mutations in FGFR3 often correlate with these conditions impacting signaling pathways and interacting with proteins such as PTPN11 highlighting its critical role in pathogenesis.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Immunoprecipitation - Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (ab155960), expandable thumbnail](https://content.abcam.com/products/images/fgfr3-phospho-y724-antibody-epr22813-ab155960--immunoprecipitation-img129595.jpg "Immunoprecipitation - Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (ab155960)")
Immunoprecipitation - Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (ab155960)
ab155960 (unpurified) at 1/15 immunoprecipitating FGFR3 (phospho Y724) in MCF7 cells treated with pervanadate. For western blotting, a peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1000).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
All lanes: Immunoprecipitation - Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (ab155960)
Predicted band size: 88 kDa
Observed band size: 98 kDa
![Western blot - Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (ab155960), expandable thumbnail](https://content.abcam.com/products/images/fgfr3-phospho-y724-antibody-epr22813-ab155960--western-blot-img24643.jpg "Western blot - Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (ab155960)")
Western blot - Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (ab155960)
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (ab155960) at 1/20000 dilution
Lane 1: MCF7 cell lysate - untreated at 10 µg
Lane 2: MCF7 cell lysate - treated with pervanadate at 10 µg
SecondaryAll lanes: Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 88 kDa
Observed band size: 96 kDa
![Immunoprecipitation - Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (ab155960), expandable thumbnail](https://content.abcam.com/products/images/fgfr3-phospho-y724-antibody-epr22813-ab155960--immunoprecipitation-img110587.jpg "Immunoprecipitation - Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (ab155960)")
Immunoprecipitation - Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (ab155960)
ab155960 (purified) at 1/50 immunoprecipitating FGFR3 (phospho Y724) in MCF7 cells treated with pervanadate. For western blotting, a peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1000).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
All lanes: Immunoprecipitation - Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (ab155960)
Predicted band size: 88 kDa
Observed band size: 98 kDa
![Flow Cytometry (Intracellular) - Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (ab155960), expandable thumbnail](https://content.abcam.com/products/images/fgfr3-phospho-y724-antibody-epr22813-ab155960--flow-cytometry-intracellular-img26290.jpg "Flow Cytometry (Intracellular) - Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (ab155960)")
Flow Cytometry (Intracellular) - Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (ab155960)
Intracellular Flow Cytometry analysis of MCF7 (human breast carcinoma) treated with 1 mM pervanadate for 30 minutes cells labeling FGFR3 (phospho Y724) with purified ab155960 at 1/50 dilution (red). The secondary antibody was Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution. Green shows untreated MCF7 (human breast carcinoma) cells. A Rabbit monoclonal IgG (Black) was used as the isotype control and cells without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.
![Immunocytochemistry/ Immunofluorescence - Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (ab155960), expandable thumbnail](https://content.abcam.com/products/images/fgfr3-phospho-y724-antibody-epr22813-ab155960--immunocytochemistry-immunofluorescence-img121559.jpg "Immunocytochemistry/ Immunofluorescence - Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (ab155960)")
Immunocytochemistry/ Immunofluorescence - Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (ab155960)
Immunocytochemistry/Immunofluorescence analysis of untreated, Per treated and Per + LP treated MCF7 cells labelling FGFR3 (phospho Y724) with ab155960 (left) and FGFR3 with ab137084 (right) both at a dilution of 1/200.
Cells were fixed with 100% methanol. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, a mouse anti-tubulin (1/1000) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.
The image shows increased cytoplasmic staining after Pervanadate (1 mM, 30 min) treatment on MCF7 cells. The LP treatment decreased the cytoplasmic staining caused by Pervanadate.
ab137084 was used as a Pan control for ab155960. The results showed cytoplasmic staining on untreated, Per treated and Per + LP treated MCF7 cells.
![Western blot - Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (ab155960), expandable thumbnail](https://content.abcam.com/products/images/fgfr3-phospho-y724-antibody-epr22813-ab155960--western-blot-img74007.jpg "Western blot - Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (ab155960)")
Western blot - Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (ab155960)
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (ab155960) at 1/10000 dilution
Lane 1: MCF7 whole cell lysate - treated with pervanadate at 10 µg
Lane 2: MCF7 whole cell lysate - untreated at 10 µg
SecondaryAll lanes: Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 88 kDa
Observed band size: 98 kDa
Exposure time: 30s
![Dot Blot - Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (ab155960), expandable thumbnail](https://content.abcam.com/products/images/fgfr3-phospho-y724-antibody-epr22813-ab155960--dot-blot-img11890.jpg "Dot Blot - Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (ab155960)")
Dot Blot - Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (ab155960)
Dot blot analysis of human FGFR3 (pY724) phospho peptide (lane 1) and human FGFR3 non-phospho peptide (lane 2) labelling FGFR2 (phospho Y724) with ab155960 at a dilution of 1/1000. A peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/2500).
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 10 seconds.
![Western blot - Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (ab155960), expandable thumbnail](https://content.abcam.com/products/images/fgfr3-phospho-y724-antibody-epr22813-ab155960--western-blot-img74007.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (ab155960)")
![Dot Blot - Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (ab155960), expandable thumbnail](https://content.abcam.com/products/images/fgfr3-phospho-y724-antibody-epr22813-ab155960--dot-blot-img11890.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-FGFR3 (phospho Y724) antibody [EPR2281(3)] (ab155960)")
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