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AB178396

Anti-FGFR4 antibody [EPR5330(N)]

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(10 Publications)

Rabbit Recombinant Monoclonal FGFR4 antibody. Suitable for WB and reacts with Human samples. Cited in 10 publications.

View Alternative Names

CD334, JTK2, TKF, FGFR4, Fibroblast growth factor receptor 4, FGFR-4

1 Images
Western blot - Anti-FGFR4 antibody [EPR5330(N)] (AB178396)
  • WB

Supplier Data

Western blot - Anti-FGFR4 antibody [EPR5330(N)] (AB178396)

The expression profile observed is consistent with what has been described in the literature PMID : 15709206.

All lanes:

Western blot - Anti-FGFR4 antibody [EPR5330(N)] (ab178396) at 1/1000 dilution

Lane 1:

SW480 cell lysate at 10 µg

Lane 2:

SH-SY5Y cell lysate at 10 µg

Lane 3:

Raji cell lysate at 10 µg

Lane 4:

MOLT4 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 88 kDa

true

  • Carrier free

    Anti-FGFR4 antibody [EPR5330(N)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5330(N)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FGFR4 or fibroblast growth factor receptor 4 is a receptor tyrosine kinase weighing approximately 110 kDa. This protein plays a role in cellular processes by binding to fibroblast growth factors. FGFR4 is expressed in many tissues including muscle liver and the developing epidermis. It is known by alternate names like CD334 or FRFR4. This receptor's activation leads to receptor dimerization and autophosphorylation triggering downstream signaling pathways that affect cell proliferation differentiation and migration.
Biological function summary

FGFR4 influences various developmental and metabolic processes. It is important in limb and skeletal development epithelial cell morphogenesis and muscle fiber regeneration. FGFR4 often partners with heparan sulfate proteoglycans to form a signaling complex which defines the specificity and regulation of fibroblast growth factor signaling. The receptor's interaction with other proteins like FRFR4 and 5b5 further modulates its biological activity.

Pathways

FGFR4 participates in the MAPK and PI3K-Akt signaling pathways both important for regulating cell survival and growth. These pathways allow FGFR4 to connect with several other proteins such as FGFR1 and BLU-554. FGFR4's activation within these pathways helps determine cellular responses to hormonal and mitogenic signals further influencing tissue homeostasis and repair mechanisms.

FGFR4 is linked to certain cancers including hepatocellular carcinoma and colorectal cancer notable in cell lines like HCT116 and others. Overexpression or mutations in FGFR4 can drive tumor progression promoting uncontrolled growth and metastasis. FGFR4's relation to other key proteins such as FRAF and 19h3 highlights its role in pathogenesis making it a potential target for therapeutic interventions. Studies involving FGFR4 ELISA kits enhance understanding of its role in disease development and progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Tyrosine-protein kinase that acts as a cell-surface receptor for fibroblast growth factors and plays a role in the regulation of cell proliferation, differentiation and migration, and in regulation of lipid metabolism, bile acid biosynthesis, glucose uptake, vitamin D metabolism and phosphate homeostasis. Required for normal down-regulation of the expression of CYP7A1, the rate-limiting enzyme in bile acid synthesis, in response to FGF19. Phosphorylates PLCG1 and FRS2. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Promotes SRC-dependent phosphorylation of the matrix protease MMP14 and its lysosomal degradation. FGFR4 signaling is down-regulated by receptor internalization and degradation; MMP14 promotes internalization and degradation of FGFR4. Mutations that lead to constitutive kinase activation or impair normal FGFR4 inactivation lead to aberrant signaling.
See full target information FGFR4
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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Journal of Cancer 15:5415-5424 PubMed39247610

2024

STAT3 inhibitor Stattic Exhibits the Synergistic Effect with FGFRs Inhibitor Erdafitinib in FGFR1-positive Lung Squamous Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Hongqin Zhong,Ling Wang,Xue Zhu,Shu Li,Xiyue Li,Chen Ding,Ke Wang,Xun Wang

British journal of cancer 131:77-89 PubMed38796598

2024

Oncofetal SNRPE promotes HCC tumorigenesis by regulating the FGFR4 expression through alternative splicing.

Applications

Unspecified application

Species

Unspecified reactive species

Qipeng Wu,Ruyan Liao,Chunmeng Miao,Muhammad Hasnat,Le Li,Lixin Sun,Xinru Wang,Ziqiao Yuan,Zhenzhou Jiang,Luyong Zhang,Qinwei Yu

Cell death & disease 15:279 PubMed38637504

2024

Cancer-associated fibroblasts secrete FGF5 to inhibit ferroptosis to decrease cisplatin sensitivity in nasopharyngeal carcinoma through binding to FGFR2.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Liu,Ling Tang,Huai Liu,Yanzhu Chen,Tengfei Xiao,Wangning Gu,Hongmin Yang,Hui Wang,Pan Chen

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e2302677 PubMed37387563

2023

Small Extracellular Vesicle-Derived vWF Induces a Positive Feedback Loop between Tumor and Endothelial Cells to Promote Angiogenesis and Metastasis in Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Samuel Wan Ki Wong,Sze Keong Tey,Xiaowen Mao,Hiu Ling Fung,Zhi-Jie Xiao,Danny Ka Ho Wong,Lung-Yi Mak,Man-Fung Yuen,Irene Oi-Lin Ng,Jing Ping Yun,Yi Gao,Judy Wai Ping Yam

Journal of inflammation research 15:6187-6197 PubMed36386580

2022

FGF15 Protects Septic Mice by Inhibiting Inflammation and Modulating Treg Responses.

Applications

Unspecified application

Species

Unspecified reactive species

Xing Li,Zexiang Zhu,Zhenkun Xia,Bo Xu

Cellular and molecular gastroenterology and hepatology 13:1161-1179 PubMed34990887

2022

Hepatic Reduction in Cholesterol 25-Hydroxylase Aggravates Diet-induced Steatosis.

Applications

Unspecified application

Species

Unspecified reactive species

Zeyu Dong,Fangzhou He,Xiaosong Yan,Yuanming Xing,Yuyang Lei,Jie Gao,Ming He,Dongmin Li,Liang Bai,Zuyi Yuan,John Y-J Shyy

Aging 13:19750-19759 PubMed34351305

2021

Dual inhibition of FGFR4 and BCL-xL inhibits multi-resistant ovarian cancer with BCL2L1 gain.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Guo,Chao Gu,Bin Li,Congjian Xu

Experimental and therapeutic medicine 19:762-770 PubMed31853327

2019

Dexamethasone and lenvatinib inhibit migration and invasion of non-small cell lung cancer by regulating EKR/AKT and VEGF signal pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Daye Zhang,Yongxiang Zhang,Zeyuan Cai,Ying Tu,Zhansong Hu

Developmental cell 48:460-474.e9 PubMed30745141

2019

FGF15 Activates Hippo Signaling to Suppress Bile Acid Metabolism and Liver Tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Suyuan Ji,Qingxu Liu,Shihao Zhang,Qinghua Chen,Cong Wang,Weiji Zhang,Chen Xiao,Yuxi Li,Cheng Nian,Jiaxin Li,Junhong Li,Jing Geng,Lixin Hong,Changchuan Xie,Ying He,Xing Chen,Xun Li,Zhen-Yu Yin,Han You,Kwang-Huei Lin,Qiao Wu,Chundong Yu,Randy L Johnson,Li Wang,Lanfen Chen,Fen Wang,Dawang Zhou

Scientific reports 6:34117 PubMed27666676

2016

Myeloid Cell-Specific Lipin-1 Deficiency Stimulates Endocrine Adiponectin-FGF15 Axis and Ameliorates Ethanol-Induced Liver Injury in Mice.

Applications

WB

Species

Mouse

Jiayou Wang,Chunki Kim,Alvin Jogasuria,Yoonhee Han,Xudong Hu,Jiashin Wu,Hong Shen,Roman Chrast,Brian N Finck,Min You
View all publications
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