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AB95940

Anti-FGFRL1 antibody

3

(1 Review)

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(7 Publications)

Rabbit Polyclonal FGFRL1 antibody. Suitable for WB, IHC-P and reacts with Transfected cell lysate, Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human FGFRL1 aa 150-500.

View Alternative Names

FGFR5, FHFR, UNQ480/PRO943, FGFRL1, Fibroblast growth factor receptor-like 1, FGF receptor-like protein 1, FGF homologous factor receptor, FGFR-like protein, Fibroblast growth factor receptor 5, FGFR-5

4 Images
Western blot - Anti-FGFRL1 antibody (AB95940)
  • WB

Supplier Data

Western blot - Anti-FGFRL1 antibody (AB95940)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-FGFRL1 antibody (ab95940) at 1/1000 dilution

Lane 1:

HepG2 whole cell lysates at 30 µg

Lane 2:

HepG2 membrane lysates at 30 µg

Secondary

All lanes:

HRP conjugated anti rabbit IgG antibody

Predicted band size: 55 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGFRL1 antibody (AB95940)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGFRL1 antibody (AB95940)

ab95940, at a 1/100 dilution, staining FGFRL1 in paraffin-embedded Oral CA Cal27 by Immunohistochemical analysis.

Western blot - Anti-FGFRL1 antibody (AB95940)
  • WB

Supplier Data

Western blot - Anti-FGFRL1 antibody (AB95940)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-FGFRL1 antibody (ab95940) at 1/1000 dilution

Lane 1:

293T whole cell lysates at 30 µg

Lane 2:

FGFRL1 transfected 293T whole cell lysates at 30 µg

Secondary

All lanes:

HRP conjugated anti rabbit IgG antibody

Predicted band size: 55 kDa

false

Western blot - Anti-FGFRL1 antibody (AB95940)
  • WB

CiteAb

Western blot - Anti-FGFRL1 antibody (AB95940)

FGFRL1 western blot using anti-FGFRL1 antibody ab95940. Publication image and figure legend from Fan, Y., Li, H., et al., 2020, Biosci Rep, PubMed 31919528.

ab95940 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab95940 please see the product overview.

Hsa-miR-107 targeted FGFRL1(A) StarBase was used to predict potential mRNA. (B) The expressions of three different types of mRNA were detected by qRT-PCR assays. (C) qRT-PCR assays were used to detect the expressions of FGFRL1 in cell lines (IMR-90, NCI-H1703, NCI-H1793 and NCI-H1869). (D) Binding sites between FGFRL1 and hsa-miR-107 were presented by bioinformatics. (E) Luciferase reporter assay was used to test the binding capacity of FGFRL1 and hsa-miR-107. (F) Overexpression efficiency of FGFRL1 was explored by qRT-PCR assays. (G) Luciferase reporter assay implemented to test the luciferase activity of wild and mutant FGD5-AS1. (H) Western blot and qRT-PCR assays were used to verify that interfered FGD5-AS1 and hsa-miR-107 can affect the expression of FGFRL1. **p<0.01 indicated statistically significant differences in this figure which n.s. indicated no significance.

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human FGFRL1 aa 150-500. The exact immunogen used to generate this antibody is proprietary information.

Q8N441

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" }, "Transfected cell lysate": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FGFRL1 also known as Fibroblast Growth Factor Receptor-Like 1 is a protein with a molecular mass of approximately 120 kDa. This protein structurally resembles other members of the fibroblast growth factor receptors but lacks the intracellular kinase domain that characterizes them. It is expressed mainly in tissues such as the heart kidney and developing skeletal system. FGFRL1 acts mechanically as a receptor binding fibroblast growth factors but differing from its relatives due to its inability to transduce signals through a conventional kinase signaling pathway.
Biological function summary

FGFRL1 plays roles in growth and development processes by modulating interactions between cells and the extracellular environment. It does not independently form a signaling complex but modulates the activity of other fibroblast growth factor receptors by acting in a regulatory capacity. FGFRL1 contributes to tissue development and differentiation by influencing cell proliferation migration and apoptosis. This regulation is essential in proper organogenesis especially involving cardiovascular structures and bones.

Pathways

FGFRL1 interacts significantly with fibroblast growth factor signaling and phosphoinositide 3-kinase (PI3K)/AKT pathways. These pathways govern processes such as cellular growth and survival. FGFRL1 modulates interactions in the fibroblast growth factor pathway with related receptors like FGFR1 to FGFR4 modulating their signal without intrinsic kinase activity. It impacts PI3K/AKT signaling indirectly by altering receptor availability and potentially affecting downstream pathway cascades.

FGFRL1 links to skeletal disorders such as craniosynostosis where premature fusion of skull bones occurs. Mutations or dysregulations in FGFRL1 disrupt normal tissue development causing abnormal bone growth. Additionally FGFRL1 has associations with cardiac malformations due to its role in heart development. Despite its distinctive activity FGFRL1's dysregulation often ties into networks involving FGFR1 to FGFR4 in these pathologies showcasing involvement in broader growth factor signaling disruptions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Has a negative effect on cell proliferation.
See full target information FGFRL1
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Experimental and therapeutic medicine 24:638 PubMed36160905

2022

HuR affects chemoresistance of small cell lung cancer by regulating FGFRL1 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Xun-Huang Duan,Rui Chen,Dao-Sheng Li,Ai-Hua Luo,Lin-Lang Guo

Cancer biology & therapy 22:372-380 PubMed34224333

2021

FGFR2 maintains cancer cell differentiation via AKT signaling in esophageal squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Osamu Maehara,Goki Suda,Mitsuteru Natsuizaka,Taku Shigesawa,Gouki Kanbe,Megumi Kimura,Masaya Sugiyama,Masashi Mizokami,Masato Nakai,Takuya Sho,Kenichi Morikawa,Koji Ogawa,Shinya Ohashi,Shingo Kagawa,Hideaki Kinugasa,Seiji Naganuma,Naoto Okubo,Shunsuke Ohnishi,Hiroshi Takeda,Naoya Sakamoto

Cell death & disease 11:731 PubMed32908121

2020

A miR-210-3p regulon that controls the Warburg effect by modulating HIF-1α and p53 activity in triple-negative breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ye Du,Na Wei,Ruolin Ma,Shuheng Jiang,Dong Song

Bioscience reports 40: PubMed31919528

2020

Long non-coding RNA FGD5-AS1 promotes non-small cell lung cancer cell proliferation through sponging hsa-miR-107 to up-regulate FGFRL1.

Applications

Unspecified application

Species

Unspecified reactive species

Yafeng Fan,Hongxia Li,Zhongping Yu,Wen Dong,Xiaoyan Cui,Jinlian Ma,Shengwen Li

Oncotarget 8:24063-24076 PubMed28445992

2017

Interaction between the estrogen receptor and fibroblast growth factor receptor pathways in non-small cell lung cancer.

Applications

WB

Species

Unspecified reactive species

Jill M Siegfried,Mariya Farooqui,Natalie J Rothenberger,Sanja Dacic,Laura P Stabile

The Prostate 76:937-47 PubMed27017949

2016

MicroRNA alteration and putative target genes in high-grade prostatic intraepithelial neoplasia and prostate cancer: STAT3 and ZEB1 are upregulated during prostate carcinogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Yoon Jin Cha,Joo Hyun Lee,Hyun Ho Han,Baek Gil Kim,Suki Kang,Young Deuk Choi,Nam Hoon Cho

Genes, chromosomes & cancer 52:860-71 PubMed23775577

2013

An integrated genomic, transcriptional and protein investigation of FGFRL1 as a putative 4p16.3 deletion target in bladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Erica di Martino,Claire F Taylor,Jo-An Roulson,Margaret A Knowles
View all publications
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