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Rabbit Recombinant Monoclonal FGL1 antibody. Suitable for IHC-P, WB and reacts with Mouse, Recombinant fragment - Mouse samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGL1 antibody [EPR29715-38] (AB324309), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGL1 antibody [EPR29715-38] (AB324309), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGL1 antibody [EPR29715-38] (AB324309), expandable thumbnail
  • Western blot - Anti-FGL1 antibody [EPR29715-38] (AB324309), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGL1 antibody [EPR29715-38] (AB324309), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWB
Mouse
Tested
Tested
Recombinant fragment - Mouse
Not recommended
Tested

Tested
Tested

Species
Mouse
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Recombinant fragment - Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Recombinant fragment - Mouse
Dilution info
1/1000
Notes

-

Target data

Function

Immune suppressive molecule that inhibits antigen-specific T-cell activation by acting as a major ligand of LAG3 (PubMed:30580966). Responsible for LAG3 T-cell inhibitory function (PubMed:30580966). Binds LAG3 independently from MHC class II (MHC-II) (PubMed:30580966). Secreted by, and promotes growth of, hepatocytes (By similarity).

Alternative names

Mfire1, Fgl1, Fibrinogen-like protein 1, Fibrinogen-related protein 1, Liver fibrinogen-related protein-1, Mfrep-1, Mfire-1

Rabbit Recombinant Monoclonal FGL1 antibody. Suitable for IHC-P, WB and reacts with Mouse, Recombinant fragment - Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR29715-38
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGL1 antibody [EPR29715-38] (ab324309), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGL1 antibody [EPR29715-38] (ab324309)

    FGL1 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Mouse squamous cell carcinoma using rabbit Anti-FGL1 antibody

    Immunohistochemical analysis of paraffin-embedded Mouse squamous cell carcinoma tissue labeling FGL1 with ab324309 at 1/100 (5.11 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on plasma of mouse squamous cell carcinoma.

    The primary antibody was incubated for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGL1 antibody [EPR29715-38] (ab324309), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGL1 antibody [EPR29715-38] (ab324309)

    FGL1 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Mouse testis using rabbit Anti-FGL1 antibody

    Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling FGL1 with ab324309 at 1/100 (5.11 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: No staining on mouse testis.

    The primary antibody was incubated for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGL1 antibody [EPR29715-38] (ab324309), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGL1 antibody [EPR29715-38] (ab324309)

    FGL1 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Mouse cerebrum using rabbit Anti-FGL1 antibody

    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling FGL1 with ab324309 at 1/100 (5.11 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: No staining on mouse cerebrum (PMID: 12528893).

    The primary antibody was incubated for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Western blot - Anti-FGL1 antibody [EPR29715-38] (ab324309), expandable thumbnail

    Western blot - Anti-FGL1 antibody [EPR29715-38] (ab324309)

    FGL1 Western blot staining using rabbit Anti-FGL1 antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This antibody does not cross-react with recombinant human FGL2 by western blot.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-FGL1 antibody [EPR29715-38] (ab324309) at 1/1000 dilution

    Lane 1: His-tagged mouse FGL1 fragment at 10 ng

    Lane 2: His-tagged mouse FGL2 fragment at 10 ng

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 30 kDa, 49 kDa

    Exposure time: 37s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGL1 antibody [EPR29715-38] (ab324309), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGL1 antibody [EPR29715-38] (ab324309)

    FGL1 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Mouse liver using rabbit Anti-FGL1 antibody

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling FGL1 with ab324309 at 1/100 (5.11 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on mouse liver (PMID: 12528893).

    The primary antibody was incubated for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Western blot - Anti-FGL1 antibody [EPR29715-38] (ab324309), expandable thumbnail

    Western blot - Anti-FGL1 antibody [EPR29715-38] (ab324309)

    FGL1 Western blot staining using rabbit Anti-FGL1 antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: brain, skeletal muscle, pancreas (PMID: 30580966; PMID: 10698685).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-FGL1 antibody [EPR29715-38] (ab324309) at 1/1000 dilution

    Lane 1: Mouse liver tissue lysate at 20 µg

    Lane 2: Mouse brain tissue lysate at 20 µg

    Lane 3: Mouse skeletal muscle tissue lysate at 20 µg

    Lane 4: Mouse pancreas tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 36 kDa

    Exposure time: 48s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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