Anti-FGL1 antibody [RM1297]

8 product images

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGL1 antibody [RM1297] (ab323611)

  FGL1 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human cerebrum tissue using rabbit Anti-FGL1 antibody

  Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling FGL1 with ab323611 at 1/2000 (0.25 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Negative control: no staining on human cerebrum.
  
  The section was incubated with ab323611 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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  Flow Cytometry - Anti-FGL1 antibody [RM1297] (ab323611)

  FGL1 Flow Cytometry staining using rabbit Anti-FGL1 antibody

  Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HepG2 (human hepatocellular carcinoma epithelial cell, Right) /HeLa (human cervical adenocarcinoma epithelial cell, Left) cells labelling FGL1 with ab323611 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

  Negative control: HeLa.

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  Immunoprecipitation - Anti-FGL1 antibody [RM1297] (ab323611)

  FGL1 was immunoprecipitated from 0.35 mg HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate with ab323611 at 1/1000 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab323611 at 1/1000 dilution.

  Blocking and dilution buffer and concentration: 5% NFDM/TBST

  All lanes: Immunoprecipitation - Anti-FGL1 antibody [RM1297] (ab323611) at 1/1000 dilution

  Lane 1: (Input) HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate

  Lane 2: ab323611 at 1/30 IP in HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate

  Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-CD37 antibody [RM1306] ab323574 in HepG2 whole cell lysate

  Secondary

  All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

  Observed band size: 34 kDa

  Exposure time: 32s

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGL1 antibody [RM1297] (ab323611)

  FGL1 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human liver cancer using rabbit Anti-FGL1 antibody

  Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue labeling FGL1 with ab323611 at 1/2000 (0.25 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining on (image A) human liver cancer, and (image B) Anti-FGL1 antibody ab197357 showed weak staining on human liver cancer.
  
  The image on (A) ab323611 is applied with Anti-FGL1 antibody at 1/2000 dilution and (B) Anti-FGL1 antibody ab197357 is applied with Anti-FGL1 antibody at 1/420 dilution.
  
  The section was incubated with ab323611 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGL1 antibody [RM1297] (ab323611)

  FGL1 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human liver tissue using rabbit Anti-FGL1 antibody

  Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling FGL1 with ab323611 at 1/2000 (0.25 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining on human liver.
  
  The section was incubated with ab323611 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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  Immunocytochemistry/ Immunofluorescence - Anti-FGL1 antibody [RM1297] (ab323611)

  FGL1 Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-FGL1 antibody

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling FGL1 with ab323611 at 1/50 (9.98 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green).

  Confocal image showing cytoplasmic staining in HepG2 cells (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).
  
  Negative control: HeLa.
  
  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

  Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.

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  Western blot - Anti-FGL1 antibody [RM1297] (ab323611)

  FGL1 Western blot staining using rabbit Anti-FGL1 antibody

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  Negative control: HeLa, Jurkat

  The molecular weight observed is consistent with what has been described in the literature (PMID: 11004478).

  In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

  All lanes: Western blot - Anti-FGL1 antibody [RM1297] (ab323611) at 1/1000 dilution

  Lane 1: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

  Lane 2: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

  Lane 3: Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Observed band size: 34 kDa, 36 kDa

  Exposure time: 180s

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  Western blot - Anti-FGL1 antibody [RM1297] (ab323611)

  FGL1 Western blot staining using rabbit Anti-FGL1 antibody

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  Negative control: cerebellum (PMID: 11470158)

  The molecular weight observed is consistent with what has been described in the literature (PMID: 11004478).

  In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

  All lanes: Western blot - Anti-FGL1 antibody [RM1297] (ab323611) at 1/1000 dilution

  Lane 1: Human liver tissue lysate at 20 µg

  Lane 2: Human liver cancer tissue lysate at 20 µg

  Lane 3: Human cerebellum tissue lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Observed band size: 34 kDa, 36 kDa

  Exposure time: 180s