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AB285039

Anti-FGL2/Prothrombinase antibody [EPR23373-38] - BSA and Azide free (Capture)

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Rabbit Recombinant Monoclonal FGL2/Prothrombinase antibody. Carrier free. Suitable for sELISA and reacts with Mouse samples.

View Alternative Names

Fibroleukin, Fibrinogen-like protein 2, pT49, FGL2

1 Images
Sandwich ELISA - Anti-FGL2/Prothrombinase antibody [EPR23373-38] - BSA and Azide free (Capture) (AB285039)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-FGL2/Prothrombinase antibody [EPR23373-38] - BSA and Azide free (Capture) (AB285039)

Example of Mouse FGL2/Prothrombinase standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23373-38

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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Product details

ab285039 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Mouse FGL2/Prothrombinase. The recommended pair for sandwich ELISA is:
Capture: ab285039, Mouse FGL2/Prothrombinase Capture Antibody (unconjugated)
Detector: ab285145, Mouse FGL2/Prothrombinase Detector Antibody (unconjugated)
The reference range value is 0.1875 - 12 ng/mL.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FGL2 also known as Fibroleukin or Prothrombinase is a protein involved in various physiological and pathological processes. Its molecular mass is approximately 70 kDa. This protein is widely expressed in different tissues including the liver and the spleen as well as in immune cells such as T cells and macrophages. It serves as an important signaling molecule and mediator in various biological pathways due to its capacity to interact with other proteins and molecular complexes.
Biological function summary

FGL2 acts in immune response modulation and blood coagulation. It can exist as a homodimer or form part of a larger protein complex. One of its roles involves cleaving prothrombin to generate thrombin contributing to the coagulation cascade. Moreover FGL2 has immunosuppressive properties affecting T cell proliferation and cytokine release. Its dual function in coagulation and immune regulation points to its importance in maintaining homeostasis and orchestrating immune responses.

Pathways

FGL2 serves key roles in both the coagulation and immune pathways. In the coagulation pathway FGL2 aids in the conversion of prothrombin to thrombin bridging innate immunity with coagulation processes. This action places it in close functional relationship with proteins in the coagulation pathway such as thrombin and fibrin. Moreover its involvement in immune pathways aligns it with cytokines and interferons modulating the immune response and maintaining immune system balance.

FGL2 plays significant roles in conditions such as viral hepatitis and certain autoimmune disorders. In viral hepatitis FGL2's upregulation contributes to liver fibrosis and inflammation often in conjunction with another protein CD147. In autoimmune disorders the dysregulation of FGL2 can lead to excessive inflammatory responses impacting proteins like the interferon-gamma. Understanding FGL2's involvement in these diseases can inform therapeutic strategies targeting both coagulation and immune dysregulation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May play a role in physiologic lymphocyte functions at mucosal sites.
See full target information FGL2
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Complementary Products

Assay Kits

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Proteins & Peptides

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com