AB110286

Anti-FH/Fumarase antibody [8F12BB5]

Name: Anti-FH/Fumarase antibody [8F12BB5] (ab110286) | Abcam
Brand: Abcam Limited
SKU: AB110286

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(2 Publications)

Mouse Monoclonal FH/Fumarase antibody. Suitable for IP, Flow Cyt, IHC-P, ICC/IF and reacts with Rat, Human, Cow samples. Cited in 2 publications.

View Alternative Names

Fumarase, HsFH, FH

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FH/Fumarase antibody [8F12BB5] (AB110286)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FH/Fumarase antibody [8F12BB5] (AB110286)

ab110286 staining FH/Fumarase in formalin-fixed, paraffin-embedded human cerebellum tissue by Immunohistochemistry at 5ug/ml. FH/Fumarase immunoactivity is most intense in neuronal cell bodies, most notably in the large Purkinje cells.

Flow Cytometry - Anti-FH/Fumarase antibody [8F12BB5] (AB110286)

Flow Cyt

Unknown

Flow Cytometry - Anti-FH/Fumarase antibody [8F12BB5] (AB110286)

HeLa cells were stained with 1 µg/mL ab110286 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.

Immunocytochemistry/ Immunofluorescence - Anti-FH/Fumarase antibody [8F12BB5] (AB110286)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-FH/Fumarase antibody [8F12BB5] (AB110286)

Immunocytochemistry image of FH/Fumarase (ab110286) stained human HDFn cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min). The cells were incubated with ab110286 at 5μg/ml for 2h at room temperature or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in mitochondria.

Unknown

Immunoprecipitation - Anti-FH/Fumarase antibody [8F12BB5] (AB110286)

FH/Fumarase immunocaptured from HepG2 cells (Lane 1), human liver mitochondria (Lane 2), bovine liver mitochondria (Lane 3), and rat liver mitochondria (Lane 4) using ab110286.

All lanes:

Immunoprecipitation - Anti-FH/Fumarase antibody [8F12BB5] (ab110286)

Predicted band size: 54 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

8F12BB5

Isotype

IgG2a

Light chain type

kappa

Carrier free

Reacts with

Rat, Cow, Human

Applications

IHC-P, Flow Cyt, ICC/IF, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg/mL", "FlowCyt-species-notes": "Recommend 0.1% Triton X-100 permeabilization.<a href='/en-us/products/primary-antibodies/mouse-igg2c-kappa-monoclonal-18c8bc7ad10-isotype-control-ab170191'>ab170191</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "Perform heat mediated antigen retrieval - 1 min pressure cook in 1mmol EDTA pH8.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form

Liquid

Purification technique

Precipitation Ammonium Sulphate

Purification notes

Purity near homogeneity as judged by SDS-PAGE. The antibody was produced in-vitro using hybridomas grown in serum-free medium and then purified by biochemical fractionation.

Storage buffer

pH: 7.5 Preservative: 0.02% Sodium azide Constituents: HEPES buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Fumarase also known as fumarate hydratase (FH) catalyzes the reversible hydration of fumarate to malate in the citric acid cycle. This enzyme weighs approximately 49 kDa. Fumarase is predominantly expressed in the mitochondria where it performs its main function. However a cytosolic form exists that plays roles outside of the citric acid cycle especially related to DNA damage response.

Biological function summary

Fumarase converts fumarate into malate which is a critical step in cellular energy production. In the mitochondria fumarase acts as a part of the TCA cycle facilitating ATP generation. In the cytosol beyond its enzymatic activity fumarase participates in DNA repair indicating its dual functionality. This suggests that fumarase might play additional roles that are yet discovered.

Pathways

Fumarase plays essential roles in both the citric acid cycle and DNA repair pathways. In the TCA cycle fumarase along with enzymes like malate dehydrogenase maintains efficient energy conversion in mitochondria. In DNA repair fumarase likely interacts with proteins such as p53 suggesting that fumarate has signaling roles impacting cell survival and replication.

Mutations in the fumarase gene associate with hereditary leiomyomatosis and renal cell cancer (HLRCC). This disorder involves impaired fumarase function leading to abnormal cellular processes and tumor development. Fumarase deficiencies also connect to neurological disorders suggesting that its dysfunction has broad effects in cellular metabolism and genome stability linking it indirectly to other proteins within the affected pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the reversible stereospecific interconversion of fumarate to L-malate (PubMed : 30761759). Experiments in other species have demonstrated that specific isoforms of this protein act in defined pathways and favor one direction over the other (Probable).. Isoform Mitochondrial. Catalyzes the hydration of fumarate to L-malate in the tricarboxylic acid (TCA) cycle to facilitate a transition step in the production of energy in the form of NADH.. Isoform Cytoplasmic. Catalyzes the dehydration of L-malate to fumarate (By similarity). Fumarate metabolism in the cytosol plays a role during urea cycle and arginine metabolism; fumarate being a by-product of the urea cycle and amino-acid catabolism (By similarity). Also plays a role in DNA repair by promoting non-homologous end-joining (NHEJ) (PubMed : 20231875, PubMed : 26237645). In response to DNA damage and phosphorylation by PRKDC, translocates to the nucleus and accumulates at DNA double-strand breaks (DSBs) : acts by catalyzing formation of fumarate, an inhibitor of KDM2B histone demethylase activity, resulting in enhanced dimethylation of histone H3 'Lys-36' (H3K36me2) (PubMed : 26237645).

See full target information FH

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Biomedicines 11: PubMed36830782

2023

TFEB Rearranged Renal Cell Carcinoma: Pathological and Molecular Characterization of 10 Cases, with Novel Clinical Implications: A Single Center 10-Year Experience.

Applications

Unspecified application

Species

Unspecified reactive species

Ai-Xiang Wang,Tai Tian,Li-Bo Liu,Feng Yang,Hui-Ying He,Li-Qun Zhou

Cancer research 79:1383-1397 PubMed30683654

2019

PAK4 Phosphorylates Fumarase and Blocks TGFβ-Induced Cell Growth Arrest in Lung Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Chen,Ting Wang,Wenhua Liang,Qin Zhao,Qiujing Yu,Chun-Min Ma,Lingang Zhuo,Dong Guo,Ke Zheng,Chengzhi Zhou,Shupei Wei,Wenhua Huang,Juhong Jiang,Jing Liu,Shiyue Li,Jianxing He,Yuhui Jiang,Nanshan Zhong

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FH/Fumarase antibody [8F12BB5]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FH/Fumarase antibody [8F12BB5] (AB110286)

Flow Cytometry - Anti-FH/Fumarase antibody [8F12BB5] (AB110286)

Immunocytochemistry/ Immunofluorescence - Anti-FH/Fumarase antibody [8F12BB5] (AB110286)

Immunoprecipitation - Anti-FH/Fumarase antibody [8F12BB5] (AB110286)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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