Goat Polyclonal FH/Fumarase antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human FH aa 150-200.
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- Nature 615:499-5062023Fumarate induces vesicular release of mtDNA to drive innate immunity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVincent Zecchini,Vincent Paupe,Irene Herranz-Montoya,Joëlle Janssen,Inge M N Wortel,Jordan L Morris,Ashley Ferguson,Suvagata Roy Chowdury,Marc Segarra-Mondejar,Ana S H Costa,Gonçalo C Pereira,Laura Tronci,Timothy Young,Efterpi Nikitopoulou,Ming Yang,Dóra Bihary,Federico Caicci,Shun Nagashima,Alyson Speed,Kalliopi Bokea,Zara Baig,Shamith Samarajiwa,Maxine Tran,Thomas Mitchell,Mark Johnson,Julien Prudent,Christian FrezzaPubMed 36890229
- Nature cell biology 17:1158-682015Local generation of fumarate promotes DNA repair through inhibition of histone H3 demethylation.Applications:WBReactive species:HumanYuhui Jiang,Xu Qian,Jianfeng Shen,Yugang Wang,Xinjian Li,Rui Liu,Yan Xia,Qianming Chen,Guang Peng,Shiaw-Yih Lin,Zhimin LuPubMed 26237645
- The EMBO journal 33:1365-822014Nuclear ARRB1 induces pseudohypoxia and cellular metabolism reprogramming in prostate cancer.Applications:WB, ICC/IFReactive species:Unspecified reactive speciesVincent Zecchini et. al.PubMed 24837709
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human FH aa 150-200. The exact immunogen used to generate this antibody is proprietary information. Database link P07954
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Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Chicken | Predicted | Predicted |
| Chinese hamster | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Dog | Predicted | Predicted |
| Horse | Predicted | Predicted |
| Pig | Predicted | Predicted |
| Sheep | Predicted | Predicted |
| Zebrafish | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.01 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Sheep, Horse, Chicken, Cow, Dog, Pig, Zebrafish, Chinese hamster, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 3.75 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Sheep, Horse, Chicken, Cow, Dog, Pig, Zebrafish, Chinese hamster, Rat | Dilution info - | Notes - |
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Target data
Function
Catalyzes the reversible stereospecific interconversion of fumarate to L-malate (PubMed:30761759). Experiments in other species have demonstrated that specific isoforms of this protein act in defined pathways and favor one direction over the other (Probable). Isoform Mitochondrial. Catalyzes the hydration of fumarate to L-malate in the tricarboxylic acid (TCA) cycle to facilitate a transition step in the production of energy in the form of NADH. Isoform Cytoplasmic. Catalyzes the dehydration of L-malate to fumarate (By similarity). Fumarate metabolism in the cytosol plays a role during urea cycle and arginine metabolism; fumarate being a by-product of the urea cycle and amino-acid catabolism (By similarity). Also plays a role in DNA repair by promoting non-homologous end-joining (NHEJ) (PubMed:20231875, PubMed:26237645). In response to DNA damage and phosphorylation by PRKDC, translocates to the nucleus and accumulates at DNA double-strand breaks (DSBs): acts by catalyzing formation of fumarate, an inhibitor of KDM2B histone demethylase activity, resulting in enhanced dimethylation of histone H3 'Lys-36' (H3K36me2) (PubMed:26237645).
Alternative names
Fumarase, HsFH, FH
Recommended products
Goat Polyclonal FH/Fumarase antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human FH aa 150-200.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human FH aa 150-200. The exact immunogen used to generate this antibody is proprietary information. Database link P07954
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Fumarase also known as fumarate hydratase (FH) catalyzes the reversible hydration of fumarate to malate in the citric acid cycle. This enzyme weighs approximately 49 kDa. Fumarase is predominantly expressed in the mitochondria where it performs its main function. However a cytosolic form exists that plays roles outside of the citric acid cycle especially related to DNA damage response.
- Biological function summary
Fumarase converts fumarate into malate which is a critical step in cellular energy production. In the mitochondria fumarase acts as a part of the TCA cycle facilitating ATP generation. In the cytosol beyond its enzymatic activity fumarase participates in DNA repair indicating its dual functionality. This suggests that fumarase might play additional roles that are yet discovered.
- Pathways
Fumarase plays essential roles in both the citric acid cycle and DNA repair pathways. In the TCA cycle fumarase along with enzymes like malate dehydrogenase maintains efficient energy conversion in mitochondria. In DNA repair fumarase likely interacts with proteins such as p53 suggesting that fumarate has signaling roles impacting cell survival and replication.
- Associated diseases and disorders
Mutations in the fumarase gene associate with hereditary leiomyomatosis and renal cell cancer (HLRCC). This disorder involves impaired fumarase function leading to abnormal cellular processes and tumor development. Fumarase deficiencies also connect to neurological disorders suggesting that its dysfunction has broad effects in cellular metabolism and genome stability linking it indirectly to other proteins within the affected pathways.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FH/Fumarase antibody (ab113963)
Immunohistochemical staining of Human Heart using ab113963 at 3.75μg/ml
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FH/Fumarase antibody (ab113963)
Immunohistochemical staining of Human Skeletal Muscle using ab113963 at 3.75μg/ml
Western blot - Anti-FH/Fumarase antibody (ab113963)
Primary incubation was 1 hour.
All lanes: Western blot - Anti-FH/Fumarase antibody (ab113963) at 0.01 µg/mL
All lanes: Human Kidney lysate at 35 µg
Developed using the ECL technique.
Predicted band size: 54 kDa
Observed band size: 48 kDa
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