AB95947

Anti-FH/Fumarase antibody

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(4 Publications)

Rabbit Polyclonal FH/Fumarase antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human FH aa 150-450.

View Alternative Names

Fumarase, HsFH, FH

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FH/Fumarase antibody (AB95947)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FH/Fumarase antibody (AB95947)

Immunohistochemical analysis of paraffin embedded NCIN87 xenograft, using ab95947 at 1/500 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-FH/Fumarase antibody (AB95947)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-FH/Fumarase antibody (AB95947)

Immunofluorescence analysis of HeLa cells fixed in ice cold MeOH for 5 min labelling Fumarate hydratase protein at mitochondria using ab95947 at a 1/500 dilution (Green). DAPI was used for nuclear staining (Blue). Scale bar = 10 µm

Supplier Data

Western blot - Anti-FH/Fumarase antibody (AB95947)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-FH/Fumarase antibody (ab95947) at 1/1000 dilution

Lane 1:

293T whole cell lysates at 30 µg

Lane 2:

A431 whole cell lysates at 30 µg

Lane 3:

HeLa whole cell lysates at 30 µg

Lane 4:

HepG2 whole cell lysates at 30 µg

Secondary

All lanes:

HRP conjugated anti rabbit IgG antibody

Predicted band size: 55 kDa

false

Supplier Data

Western blot - Anti-FH/Fumarase antibody (AB95947)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-FH/Fumarase antibody (ab95947) at 1/5000 dilution

All lanes:

Rat liver tissue lysates at 50 µg

Secondary

All lanes:

HRP conjugated anti rabbit IgG antibody

Predicted band size: 55 kDa

false

Supplier Data

Western blot - Anti-FH/Fumarase antibody (AB95947)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-FH/Fumarase antibody (ab95947) at 1/1000 dilution

All lanes:

Mouse liver tissue lysates at 50 µg

Secondary

All lanes:

HRP conjugated anti rabbit IgG antibody

Predicted band size: 55 kDa

false

Supplier Data

Western blot - Anti-FH/Fumarase antibody (AB95947)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-FH/Fumarase antibody (ab95947) at 1/1000 dilution

Lane 1:

Non-transfected (-) 293T whole cell extracts at 30 µg

Lanes 2 - 3:

FH shRNA transfected (+) 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 55 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human FH aa 150-450. The exact immunogen used to generate this antibody is proprietary information.

P07954

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Fumarase also known as fumarate hydratase (FH) catalyzes the reversible hydration of fumarate to malate in the citric acid cycle. This enzyme weighs approximately 49 kDa. Fumarase is predominantly expressed in the mitochondria where it performs its main function. However a cytosolic form exists that plays roles outside of the citric acid cycle especially related to DNA damage response.

Biological function summary

Fumarase converts fumarate into malate which is a critical step in cellular energy production. In the mitochondria fumarase acts as a part of the TCA cycle facilitating ATP generation. In the cytosol beyond its enzymatic activity fumarase participates in DNA repair indicating its dual functionality. This suggests that fumarase might play additional roles that are yet discovered.

Pathways

Fumarase plays essential roles in both the citric acid cycle and DNA repair pathways. In the TCA cycle fumarase along with enzymes like malate dehydrogenase maintains efficient energy conversion in mitochondria. In DNA repair fumarase likely interacts with proteins such as p53 suggesting that fumarate has signaling roles impacting cell survival and replication.

Mutations in the fumarase gene associate with hereditary leiomyomatosis and renal cell cancer (HLRCC). This disorder involves impaired fumarase function leading to abnormal cellular processes and tumor development. Fumarase deficiencies also connect to neurological disorders suggesting that its dysfunction has broad effects in cellular metabolism and genome stability linking it indirectly to other proteins within the affected pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the reversible stereospecific interconversion of fumarate to L-malate (PubMed : 30761759). Experiments in other species have demonstrated that specific isoforms of this protein act in defined pathways and favor one direction over the other (Probable).. Isoform Mitochondrial. Catalyzes the hydration of fumarate to L-malate in the tricarboxylic acid (TCA) cycle to facilitate a transition step in the production of energy in the form of NADH.. Isoform Cytoplasmic. Catalyzes the dehydration of L-malate to fumarate (By similarity). Fumarate metabolism in the cytosol plays a role during urea cycle and arginine metabolism; fumarate being a by-product of the urea cycle and amino-acid catabolism (By similarity). Also plays a role in DNA repair by promoting non-homologous end-joining (NHEJ) (PubMed : 20231875, PubMed : 26237645). In response to DNA damage and phosphorylation by PRKDC, translocates to the nucleus and accumulates at DNA double-strand breaks (DSBs) : acts by catalyzing formation of fumarate, an inhibitor of KDM2B histone demethylase activity, resulting in enhanced dimethylation of histone H3 'Lys-36' (H3K36me2) (PubMed : 26237645).

See full target information FH

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Clinical cancer research : an official journal of the American Association for Cancer Research 26:391-396 PubMed31636096

2019

Fumarate Metabolic Signature for the Detection of Reed Syndrome in Humans.

Applications

Unspecified application

Species

Unspecified reactive species

Ruth T Casey,Mary A McLean,Benjamin G Challis,Terri P McVeigh,Anne Y Warren,Lee Mendil,Richard Houghton,Stefano De Sanctis,Vasilis Kosmoliaptsis,Richard N Sandford,Ferdia A Gallagher,Eamonn R Maher

Molecular genetics and metabolism 128:444-451 PubMed31648943

2019

Tricarboxylic acid cycle enzyme activities in a mouse model of methylmalonic aciduria.

Applications

Unspecified application

Species

Unspecified reactive species

Parith Wongkittichote,Gary Cunningham,Marshall L Summar,Elena Pumbo,Patrick Forny,Matthias R Baumgartner,Kimberly A Chapman

Pathology 51:659-663 PubMed31481265

2019

Concordance of p16, FH, and alpha-SMA expression with the fumarate hydratase gene mutational status in sporadic and hereditary piloleiomyomas.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Cristina Gironi,Lara Camillo,Francesca Zottarelli,Paola Savoia

Proceedings of the National Academy of Sciences of 113:10998-1003 PubMed27621446

2016

E4F1 controls a transcriptional program essential for pyruvate dehydrogenase activity.

Applications

Unspecified application

Species

Unspecified reactive species

Matthieu Lacroix,Geneviève Rodier,Olivier Kirsh,Thibault Houles,Hélène Delpech,Berfin Seyran,Laurie Gayte,Francois Casas,Laurence Pessemesse,Maud Heuillet,Floriant Bellvert,Jean-Charles Portais,Charlene Berthet,Florence Bernex,Michele Brivet,Audrey Boutron,Laurent Le Cam,Claude Sardet

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-FH/Fumarase antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FH/Fumarase antibody (AB95947)

Immunocytochemistry/ Immunofluorescence - Anti-FH/Fumarase antibody (AB95947)

Western blot - Anti-FH/Fumarase antibody (AB95947)

Western blot - Anti-FH/Fumarase antibody (AB95947)

Western blot - Anti-FH/Fumarase antibody (AB95947)

Western blot - Anti-FH/Fumarase antibody (AB95947)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise