AB95950

Anti-FH/Fumarase antibody

Name: Anti-FH/Fumarase antibody (ab95950) | Abcam
Brand: Abcam Limited
SKU: AB95950

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(4 Publications)

Rabbit Polyclonal FH/Fumarase antibody. Suitable for ICC/IF, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human FH aa 50-350.

View Alternative Names

Fumarase, HsFH, FH

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FH/Fumarase antibody (AB95950)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FH/Fumarase antibody (AB95950)

ab95950 at a 1 : 500 dilution, staining human FH/Fumarase in OVCAR3 xenograft, using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue.

Immunocytochemistry/ Immunofluorescence - Anti-FH/Fumarase antibody (AB95950)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-FH/Fumarase antibody (AB95950)

Immunofluorescence analysis of HeLa cells fixed in ice-cold MeOH for 5 min labelling Fumarate hydratase protein at mitochondria using ab95950 at a 1/500 dilution (Green). Hoechst 33342 was used for nuclear staining (Blue).
Scale bar = 10 µM

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Western blot - Anti-FH/Fumarase antibody (AB95950)

All lanes:

Western blot - Anti-FH/Fumarase antibody (ab95950) at 1/5000 dilution

Lane 1:

293T whole cell lysate at 30 µg

Lane 2:

A431 whole cell lysate at 30 µg

Predicted band size: 54 kDa

false

Supplier Data

Western blot - Anti-FH/Fumarase antibody (AB95950)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-FH/Fumarase antibody (ab95950) at 1/5000 dilution

Lane 1:

293T whole cell extracts at 30 µg

Lane 2:

FH shRNA1 transfected 293T whole cell extracts at 30 µg

Lane 3:

FH shRNA2 transfected 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 55 kDa

false

Supplier Data

Western blot - Anti-FH/Fumarase antibody (AB95950)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-FH/Fumarase antibody (ab95950) at 1/5000 dilution

All lanes:

rat liver lysate at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 55 kDa

false

Supplier Data

Western blot - Anti-FH/Fumarase antibody (AB95950)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-FH/Fumarase antibody (ab95950) at 1/1000 dilution

All lanes:

mouse liver lysate at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 55 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human FH aa 50-350. The exact immunogen used to generate this antibody is proprietary information.

P07954

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Fumarase also known as fumarate hydratase (FH) catalyzes the reversible hydration of fumarate to malate in the citric acid cycle. This enzyme weighs approximately 49 kDa. Fumarase is predominantly expressed in the mitochondria where it performs its main function. However a cytosolic form exists that plays roles outside of the citric acid cycle especially related to DNA damage response.

Biological function summary

Fumarase converts fumarate into malate which is a critical step in cellular energy production. In the mitochondria fumarase acts as a part of the TCA cycle facilitating ATP generation. In the cytosol beyond its enzymatic activity fumarase participates in DNA repair indicating its dual functionality. This suggests that fumarase might play additional roles that are yet discovered.

Pathways

Fumarase plays essential roles in both the citric acid cycle and DNA repair pathways. In the TCA cycle fumarase along with enzymes like malate dehydrogenase maintains efficient energy conversion in mitochondria. In DNA repair fumarase likely interacts with proteins such as p53 suggesting that fumarate has signaling roles impacting cell survival and replication.

Mutations in the fumarase gene associate with hereditary leiomyomatosis and renal cell cancer (HLRCC). This disorder involves impaired fumarase function leading to abnormal cellular processes and tumor development. Fumarase deficiencies also connect to neurological disorders suggesting that its dysfunction has broad effects in cellular metabolism and genome stability linking it indirectly to other proteins within the affected pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the reversible stereospecific interconversion of fumarate to L-malate (PubMed : 30761759). Experiments in other species have demonstrated that specific isoforms of this protein act in defined pathways and favor one direction over the other (Probable).. Isoform Mitochondrial. Catalyzes the hydration of fumarate to L-malate in the tricarboxylic acid (TCA) cycle to facilitate a transition step in the production of energy in the form of NADH.. Isoform Cytoplasmic. Catalyzes the dehydration of L-malate to fumarate (By similarity). Fumarate metabolism in the cytosol plays a role during urea cycle and arginine metabolism; fumarate being a by-product of the urea cycle and amino-acid catabolism (By similarity). Also plays a role in DNA repair by promoting non-homologous end-joining (NHEJ) (PubMed : 20231875, PubMed : 26237645). In response to DNA damage and phosphorylation by PRKDC, translocates to the nucleus and accumulates at DNA double-strand breaks (DSBs) : acts by catalyzing formation of fumarate, an inhibitor of KDM2B histone demethylase activity, resulting in enhanced dimethylation of histone H3 'Lys-36' (H3K36me2) (PubMed : 26237645).

See full target information FH

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Science advances 11:eadq0334 PubMed40249802

2025

Targeting PGM3 abolishes SREBP-1 activation-hexosamine synthesis feedback regulation to effectively suppress brain tumor growth.

Applications

Unspecified application

Species

Unspecified reactive species

Huali Su,Yaogang Zhong,Liqing He,Feng Geng,Xinmin Yin,Yongjun Kou,Cheng-Yao Chiang,Xiaokui Mo,Yunzhou Fan,Yanwei Liu,Qiang Wang,Shino Magaki,Timothy F Cloughesy,Etienne Lefai,William H Yong,Arnab Chakravarti,Xiang Zhang,Deliang Guo

Oncotarget 13:1054-1067 PubMed36128328

2022

Targeting Krebs-cycle-deficient renal cell carcinoma with Poly ADP-ribose polymerase inhibitors and low-dose alkylating chemotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Daiki Ueno,Juan C Vasquez,Amrita Sule,Jiayu Liang,Jinny van Doorn,Ranjini Sundaram,Sam Friedman,Randy Caliliw,Shinji Ohtake,Xun Bao,Jing Li,Huihui Ye,Karla Boyd,Rong Rong Huang,Jack Dodson,Paul Boutros,Ranjit S Bindra,Brian Shuch

Multiple sclerosis (Houndmills, Basingstoke, England) 28:1179-1188 PubMed34841955

2021

Reduced expression of mitochondrial fumarate hydratase in progressive multiple sclerosis contributes to impaired in vitro mesenchymal stromal cell-mediated neuroprotection.

Applications

Unspecified application

Species

Unspecified reactive species

Pamela Sarkar,Juliana Redondo,Kelly Hares,Steven Bailey,Anastasia Georgievskaya,Kate Heesom,Kevin C Kemp,Neil J Scolding,Claire M Rice

International journal of molecular sciences 22: PubMed33799686

2021

Prolyl Hydroxylase 3 Knockdown Accelerates -Mutant Kidney Cancer Growth In Vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Niki M Zacharias,Lei Wang,Tapati Maity,Li Li,Steven W Millward,Jose A Karam,Christopher G Wood,Neema Navai

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FH/Fumarase antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FH/Fumarase antibody (AB95950)

Immunocytochemistry/ Immunofluorescence - Anti-FH/Fumarase antibody (AB95950)

Western blot - Anti-FH/Fumarase antibody (AB95950)

Western blot - Anti-FH/Fumarase antibody (AB95950)

Western blot - Anti-FH/Fumarase antibody (AB95950)

Western blot - Anti-FH/Fumarase antibody (AB95950)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise