Anti-FH/Fumarase antibody [EPR11647(2)(B)] - BSA and Azide free

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-FH/Fumarase antibody [EPR11647(2)(B)] - BSA and Azide free (AB250784)

This data was developed using ab184945, the same antibody clone in a different buffer formulation.

Immunofluorescence analysis of, -20 °C acetone-fixed, HeLa cells labeling FH/Fumarase with ab184945 at a 1/100 dilution. As secondary antibody goat anti-rabbit IgG (Alexa Fluor®488) was used at a 1/200. In blue DAPI staining.

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-FH/Fumarase antibody [EPR11647(2)(B)] - BSA and Azide free (AB250784)

This data was developed using ab184945, the same antibody clone in a different buffer formulation.

Intracellular Flow Cytometry analysis of 2% paraformaldehyde-fixed HeLa cells labeling FH/Fumarase with ab184945 at a 1/160 dilution (red) or negative control rabbit IgG (green). Secondary antibody goat anti-rabbit IgG (FITC) at a 1/150 dilution.

• WB

Supplier Data

Western blot - Anti-FH/Fumarase antibody [EPR11647(2)(B)] - BSA and Azide free (AB250784)

This data was developed using ab184945, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-FH/Fumarase antibody [EPR11647(2)(B)] - N-terminal (<a href='/en-us/products/primary-antibodies/fh-fumarase-antibody-epr116472b-n-terminal-ab184945'>ab184945</a>) at 1/2000 dilution

Lane 1:

HepG2 cell lysate at 20 µg

Lane 2:

HeLa cell lysate at 20 µg

Lane 3:

293 cell lysate at 20 µg

Lane 4:

A549 cell lysate at 20 µg

Secondary

All lanes:

goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 54 kDa

Observed band size: 49 kDa

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