Rabbit Recombinant Monoclonal FHL1 antibody. Carrier free. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Mouse, Human, Rat samples.
Images
![Western blot - Anti-FHL1 antibody [EPR22842-95] - BSA and Azide free (AB256551), expandable thumbnail](https://content.abcam.com/products/images/fhl1-antibody-epr22842-95-bsa-and-azide-free-ab256551--western-blot-img14139.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-FHL1 antibody [EPR22842-95] - BSA and Azide free (AB256551)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FHL1 antibody [EPR22842-95] - BSA and Azide free (AB256551), expandable thumbnail](https://content.abcam.com/products/images/fhl1-antibody-epr22842-95-bsa-and-azide-free-ab256551--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img124912.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FHL1 antibody [EPR22842-95] - BSA and Azide free (AB256551)")
![Immunoprecipitation - Anti-FHL1 antibody [EPR22842-95] - BSA and Azide free (AB256551), expandable thumbnail](https://content.abcam.com/products/images/fhl1-antibody-epr22842-95-bsa-and-azide-free-ab256551--immunoprecipitation-img4105.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-FHL1 antibody [EPR22842-95] - BSA and Azide free (AB256551)")
![Immunocytochemistry/ Immunofluorescence - Anti-FHL1 antibody [EPR22842-95] - BSA and Azide free (AB256551), expandable thumbnail](https://content.abcam.com/products/images/fhl1-antibody-epr22842-95-bsa-and-azide-free-ab256551--immunocytochemistry-immunofluorescence-img127251.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-FHL1 antibody [EPR22842-95] - BSA and Azide free (AB256551)")
![Immunoprecipitation - Anti-FHL1 antibody [EPR22842-95] - BSA and Azide free (AB256551), expandable thumbnail](https://content.abcam.com/products/images/fhl1-antibody-epr22842-95-bsa-and-azide-free-ab256551--immunoprecipitation-img4295.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-FHL1 antibody [EPR22842-95] - BSA and Azide free (AB256551)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | ICC/IF | IHC-P | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Tested | Tested | Tested |
| Mouse | Tested | Not recommended | Tested | Expected | Tested |
| Rat | Expected | Not recommended | Expected | Expected | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
May have an involvement in muscle development or hypertrophy.
Alternative names
SLIM1, FHL1, Four and a half LIM domains protein 1, FHL-1, Skeletal muscle LIM-protein 1, SLIM, SLIM-1
Recommended products
Rabbit Recombinant Monoclonal FHL1 antibody. Carrier free. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Mouse, Human, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR22842-95
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab256551 is the carrier-free version of Anti-FHL1 antibody [EPR22842-95] ab255828.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The FHL1 protein also known as Four and a Half LIM Domains 1 operates mechanically as a multifunctional adaptor protein. Its molecular mass is approximately 35 kDa. This protein is widely expressed in skeletal muscle and heart tissues playing a role in various cellular contexts. FHL1 interacts within the cell cytoplasm and nucleus allowing it to assist in multiple cellular processes.
- Biological function summary
FHL1 integrates into diverse cellular mechanisms by engaging in protein-protein interactions. It acts as part of complexes that regulate transcription cell differentiation and development. The involvement of FHL1 in muscle growth and maintenance is significant partly due to its expression pattern. In skeletal muscles it helps adapt muscle response to mechanical stress while in cardiac tissue it participates in muscle contractility and integrity.
- Pathways
FHL1 plays important roles in both the muscle differentiation pathway and the MAPK/ERK signaling pathway. It influences the transcriptional regulation essential for muscle cell growth and differentiation. FHL1 interacts with proteins such as MyoD during muscle formation and MEF2 in the MAPK/ERK pathway modulating cellular responses to external signals and stressors.
- Associated diseases and disorders
FHL1's involvement is evident in conditions such as Emery-Dreifuss muscular dystrophy and cardiomyopathy. Mutations or dysregulation of this protein can impair muscle function leading to myopathy. It associates with other proteins like emerin in genetic disorders affecting muscle tissues and structural integrity revealing its vital role in maintaining muscular health.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
9 product images
![Western blot - Anti-FHL1 antibody [EPR22842-95] - BSA and Azide free (ab256551), expandable thumbnail](https://content.abcam.com/products/images/fhl1-antibody-epr22842-95-bsa-and-azide-free-ab256551--western-blot-img14139.jpg "Western blot - Anti-FHL1 antibody [EPR22842-95] - BSA and Azide free (ab256551)")
Western blot - Anti-FHL1 antibody [EPR22842-95] - BSA and Azide free (ab256551)
This data was developed using Anti-FHL1 antibody [EPR22842-95] ab255828, the same antibody clone in a different buffer formulation.
Lanes 1-4: Merged signal (red and green). Green - Anti-FHL1 antibody [EPR22842-95] ab255828 observed at 32 kDa. Red - loading control Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 observed at 50 kDa.
Anti-FHL1 antibody [EPR22842-95] ab255828 Anti-FHL1 antibody [EPR22842-95] was shown to specifically react with FHL1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human FHL1 knockout HeLa cell line ab266011 (knockout cell lysate Human FHL1 knockout HeLa cell lysate ab257952) was used. Wild-type and FHL1 knockout samples were subjected to SDS-PAGE. Anti-FHL1 antibody [EPR22842-95] ab255828 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-FHL1 antibody [EPR22842-95] (Anti-FHL1 antibody [EPR22842-95] ab255828) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: FHL1 knockout HeLa cell lysate at 20 µg
Lane 2: Western blot - Human FHL1 knockout HeLa cell line (Human FHL1 knockout HeLa cell line ab266011)
Lane 3: HEK293T cell lysate at 20 µg
Lane 4: Daudi cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution
Predicted band size: 36 kDa
Observed band size: 32 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FHL1 antibody [EPR22842-95] - BSA and Azide free (ab256551), expandable thumbnail](https://content.abcam.com/products/images/fhl1-antibody-epr22842-95-bsa-and-azide-free-ab256551--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img124912.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FHL1 antibody [EPR22842-95] - BSA and Azide free (ab256551)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FHL1 antibody [EPR22842-95] - BSA and Azide free (ab256551)
Immunohistochemical analysis of paraffin-embedded human colon tissue labeling FHL1 with Anti-FHL1 antibody [EPR22842-95] ab255828 at 1/500 dilution (1.036 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on the stroma and smooth muscle in human colon is observed. The section was incubated with Anti-FHL1 antibody [EPR22842-95] ab255828 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-FHL1 antibody [EPR22842-95] ab255828).
![Immunoprecipitation - Anti-FHL1 antibody [EPR22842-95] - BSA and Azide free (ab256551), expandable thumbnail](https://content.abcam.com/products/images/fhl1-antibody-epr22842-95-bsa-and-azide-free-ab256551--immunoprecipitation-img4105.jpg "Immunoprecipitation - Anti-FHL1 antibody [EPR22842-95] - BSA and Azide free (ab256551)")
Immunoprecipitation - Anti-FHL1 antibody [EPR22842-95] - BSA and Azide free (ab256551)
FHL1 was immunoprecipitated from 0.35 mg mouse skeletal muscle lysate 10μg with Anti-FHL1 antibody [EPR22842-95] ab255828 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-FHL1 antibody [EPR22842-95] ab255828 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Mouse skeletal muscle lysate 10μg.
Lane 2: Anti-FHL1 antibody [EPR22842-95] ab255828 IP in mouse skeletal muscle lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-FHL1 antibody [EPR22842-95] ab255828 in mouse skeletal muscle lysate.
Blocking and dilution buffer and concentration/ 5% NFDM/TBST.
Exposure time/ 30 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-FHL1 antibody [EPR22842-95] ab255828).
All lanes: Immunoprecipitation - Anti-FHL1 antibody [EPR22842-95] (Anti-FHL1 antibody [EPR22842-95] ab255828)
Predicted band size: 36 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-FHL1 antibody [EPR22842-95] - BSA and Azide free (ab256551), expandable thumbnail](https://content.abcam.com/products/images/fhl1-antibody-epr22842-95-bsa-and-azide-free-ab256551--immunocytochemistry-immunofluorescence-img127251.jpg "Immunocytochemistry/ Immunofluorescence - Anti-FHL1 antibody [EPR22842-95] - BSA and Azide free (ab256551)")
Immunocytochemistry/ Immunofluorescence - Anti-FHL1 antibody [EPR22842-95] - BSA and Azide free (ab256551)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NCI-H1299 (Human lung carcinoma epithelial cell) cells labeling FHL1 with Anti-FHL1 antibody [EPR22842-95] ab255828 at 1/50 10 μg/ml dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 2 μg/ml dilution (Green). Confocal image showing cytoplasmic and nuclear staining in NCI-H1299 cell line. Low expression control: NCI-H460 cell line (PMID: 21702045). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 2 μg/ml dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-FHL1 antibody [EPR22842-95] ab255828).
![Immunoprecipitation - Anti-FHL1 antibody [EPR22842-95] - BSA and Azide free (ab256551), expandable thumbnail](https://content.abcam.com/products/images/fhl1-antibody-epr22842-95-bsa-and-azide-free-ab256551--immunoprecipitation-img4295.jpg "Immunoprecipitation - Anti-FHL1 antibody [EPR22842-95] - BSA and Azide free (ab256551)")
Immunoprecipitation - Anti-FHL1 antibody [EPR22842-95] - BSA and Azide free (ab256551)
FHL1 was immunoprecipitated from 0.35 mg human lung lysate 10μg with Anti-FHL1 antibody [EPR22842-95] ab255828 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-FHL1 antibody [EPR22842-95] ab255828 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Human lung lysate 10μg.
Lane 2: Anti-FHL1 antibody [EPR22842-95] ab255828 IP in human lung lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-FHL1 antibody [EPR22842-95] ab255828 in human lung lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time/ 30 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-FHL1 antibody [EPR22842-95] ab255828).
All lanes: Immunoprecipitation - Anti-FHL1 antibody [EPR22842-95] (Anti-FHL1 antibody [EPR22842-95] ab255828)
Predicted band size: 36 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FHL1 antibody [EPR22842-95] - BSA and Azide free (ab256551), expandable thumbnail](https://content.abcam.com/products/images/fhl1-antibody-epr22842-95-bsa-and-azide-free-ab256551--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img78365.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FHL1 antibody [EPR22842-95] - BSA and Azide free (ab256551)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FHL1 antibody [EPR22842-95] - BSA and Azide free (ab256551)
Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling FHL1 with Anti-FHL1 antibody [EPR22842-95] ab255828 at 1/500 dilution (1.036 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Cytoplasmic staining on rat skeletal muscle (PMID: 27443559, 9714789) is observed. The section was incubated with Anti-FHL1 antibody [EPR22842-95] ab255828 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-FHL1 antibody [EPR22842-95] ab255828).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FHL1 antibody [EPR22842-95] - BSA and Azide free (ab256551), expandable thumbnail](https://content.abcam.com/products/images/fhl1-antibody-epr22842-95-bsa-and-azide-free-ab256551--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img70962.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FHL1 antibody [EPR22842-95] - BSA and Azide free (ab256551)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FHL1 antibody [EPR22842-95] - BSA and Azide free (ab256551)
Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling FHL1 with Anti-FHL1 antibody [EPR22842-95] ab255828 at 1/500 dilution (1.036 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Cytoplasmic staining on mouse lung (PMID: 29067108) is observed. The section was incubated with Anti-FHL1 antibody [EPR22842-95] ab255828 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-FHL1 antibody [EPR22842-95] ab255828).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FHL1 antibody [EPR22842-95] - BSA and Azide free (ab256551), expandable thumbnail](https://content.abcam.com/products/images/fhl1-antibody-epr22842-95-bsa-and-azide-free-ab256551--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img77280.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FHL1 antibody [EPR22842-95] - BSA and Azide free (ab256551)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FHL1 antibody [EPR22842-95] - BSA and Azide free (ab256551)
Immunohistochemical analysis of paraffin-embedded human cardiac muscle tissue labeling FHL1 with Anti-FHL1 antibody [EPR22842-95] ab255828 at 1/500 dilution (1.036 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Cytoplasmic staining on human cardiac muscle (PMID: 9714789) is observed. The section was incubated with Anti-FHL1 antibody [EPR22842-95] ab255828 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-FHL1 antibody [EPR22842-95] ab255828).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FHL1 antibody [EPR22842-95] - BSA and Azide free (ab256551), expandable thumbnail](https://content.abcam.com/products/images/fhl1-antibody-epr22842-95-bsa-and-azide-free-ab256551--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img44510.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FHL1 antibody [EPR22842-95] - BSA and Azide free (ab256551)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FHL1 antibody [EPR22842-95] - BSA and Azide free (ab256551)
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling FHL1 with Anti-FHL1 antibody [EPR22842-95] ab255828 at 1/500 dilution (1.036 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Cytoplasmic staining on human skeletal muscle (PMID: 27443559, 9714789) is observed. The section was incubated with Anti-FHL1 antibody [EPR22842-95] ab255828 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-FHL1 antibody [EPR22842-95] ab255828).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FHL1 antibody [EPR22842-95] - BSA and Azide free (ab256551), expandable thumbnail](https://content.abcam.com/products/images/fhl1-antibody-epr22842-95-bsa-and-azide-free-ab256551--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img78365.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FHL1 antibody [EPR22842-95] - BSA and Azide free (ab256551)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FHL1 antibody [EPR22842-95] - BSA and Azide free (ab256551), expandable thumbnail](https://content.abcam.com/products/images/fhl1-antibody-epr22842-95-bsa-and-azide-free-ab256551--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img70962.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FHL1 antibody [EPR22842-95] - BSA and Azide free (ab256551)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FHL1 antibody [EPR22842-95] - BSA and Azide free (ab256551), expandable thumbnail](https://content.abcam.com/products/images/fhl1-antibody-epr22842-95-bsa-and-azide-free-ab256551--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img77280.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FHL1 antibody [EPR22842-95] - BSA and Azide free (ab256551)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FHL1 antibody [EPR22842-95] - BSA and Azide free (ab256551), expandable thumbnail](https://content.abcam.com/products/images/fhl1-antibody-epr22842-95-bsa-and-azide-free-ab256551--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img44510.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FHL1 antibody [EPR22842-95] - BSA and Azide free (ab256551)")
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