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AB12327

Anti-FHL2 antibody

5

(3 Reviews)

|

(13 Publications)

Rabbit Polyclonal FHL2 antibody. Suitable for WB and reacts with Human samples. Cited in 13 publications. Immunogen corresponding to Synthetic Peptide within Human FHL2 aa 150-250.

View Alternative Names

DRAL, SLIM3, FHL2, Four and a half LIM domains protein 2, FHL-2, LIM domain protein DRAL, Skeletal muscle LIM-protein 3, SLIM-3

2 Images
Western blot - Anti-FHL2 antibody (AB12327)
  • WB

Lab

Western blot - Anti-FHL2 antibody (AB12327)

Lanes 1 - 3 : Merged signal (red and green). Green - ab12327 observed at 32 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A] observed at 55kDa.

ab12327 was shown to react with FHL2 in wild-type U-2 OS cells in western blot with loss of signal observed in FHL2 knockout sample. Wild-type and FHL2 knockout U-2 OS cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab12327 and ab7291 (Mouse anti-Alpha Tubulin [DM1A] overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-FHL2 antibody (ab12327) at 1/1000 dilution

Lane 1:

Wild-type U-2 OS cell lysate at 40 µg

Lane 2:

FHL2 knockout U-2 OS cell lysate at 40 µg

Lane 3:

HeLa cell lysate at 40 µg

Predicted band size: 32 kDa

Observed band size: 32 kDa

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Western blot - Anti-FHL2 antibody (AB12327)
  • WB

Unknown

Western blot - Anti-FHL2 antibody (AB12327)

Samples : Nuclear extract (NE) from HeLa cells. Antibody : Affinity purified rabbit anti-FHL2 antibody ab12327 used at 0.3 μg/ml. Detection : Chemiluminescence with an exposure time of 5 minutes.

Samples : Nuclear extract (NE) from HeLa cells. Antibody : Affinity purified rabbit anti-FHL2 antibody ab12327 used at 0.3 µg/ml. Detection : Chemiluminescence with an exposure time of 5 minutes.

All lanes:

Western blot - Anti-FHL2 antibody (ab12327)

Predicted band size: 32 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human FHL2 aa 150-250. The exact immunogen used to generate this antibody is proprietary information.

Q14192

Specificity

Reactivity with mouse FHL2 is unlikely owing to divergence of this ortholog from the peptide used for antibody production. The epitope recognized by ab1237 maps to a region between residues 175 and 225 of human Four and a Half LIM domains 2 using the numbering given in entry NP_963849.1 (GeneID 2274).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
This antibody is affinity purified.
Storage buffer
pH: 7 - 8 Preservative: 0.1% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The FHL2 protein also known as Four and a Half LIM Domains Protein 2 plays an important role in cellular mechanics. Its molecular mass is approximately 32 kDa. FHL2 is expressed in various tissues with significant presence in cardiac and skeletal muscles as well as the liver. It is involved in the regulation of gene expression by binding to other proteins and DNA through its LIM domains. This ability allows FHL2 to influence cellular structures and functions integrally.
Biological function summary

The FHL2 protein serves critical functions in muscle development and differentiation. It operates as part of a multiprotein complex that interacts with cytoskeletal components contributing importantly to muscle cell adhesion and communication. Moreover FHL2 engages in transcriptional regulation and acts as a coactivator or corepressor of specific transcription factors influencing gene expression patterns relevant to muscle function and maintenance.

Pathways

The FHL2 protein plays significant roles in Wnt and MAPK signaling pathways. In the Wnt signaling pathway FHL2 regulates the transcriptional activity of beta-catenin an essential component of the pathway involved in cell growth and differentiation. Additionally FHL2 contributes to the MAPK signaling pathway by influencing interactions between upstream regulators and downstream targets modulating cellular responses to external stimuli. Through these pathways FHL2 interacts with various proteins including GATA4 and TEF-1 which are vital for its regulatory functions.

FHL2 has associations with certain cancers like prostate cancer and cardiomyopathy. In prostate cancer FHL2 overexpression leads to increased tumor progression and is often linked to androgen receptors promoting malignant transformation. In cardiomyopathy alterations in FHL2 expression affect heart muscle integrity and function sometimes in conjunction with interactions involving desmin an important structural protein. Understanding FHL2's connections to these diseases offers insights into potential therapeutic targets.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May function as a molecular transmitter linking various signaling pathways to transcriptional regulation. Negatively regulates the transcriptional repressor E4F1 and may function in cell growth. Inhibits the transcriptional activity of FOXO1 and its apoptotic function by enhancing the interaction of FOXO1 with SIRT1 and FOXO1 deacetylation. Negatively regulates the calcineurin/NFAT signaling pathway in cardiomyocytes (PubMed : 28717008).
See full target information FHL2
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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

International journal of experimental pathology 104:64-75 PubMed36694990

2023

Synergistic activation of genes promoting invasiveness by dual deprivation in oxygen and nutrients.

Applications

Unspecified application

Species

Unspecified reactive species

Charly Jehanno,Yann Le Page,Gilles Flouriot,Pascale Le Goff,Denis Michel

International journal of molecular sciences 24: PubMed36614145

2022

E3 Ubiquitin Ligase Midline 1 Regulates Endothelial Cell ICAM-1 Expression and Neutrophil Adhesion in Abdominal Sepsis.

Applications

Unspecified application

Species

Unspecified reactive species

Feifei Du,Avin Hawez,Zhiyi Ding,Yongzhi Wang,Carl-Fredrik Rönnow,Milladur Rahman,Henrik Thorlacius

Oncology letters 22:637 PubMed34295384

2021

Targeting FHL2-E-cadherin axis by miR-340-5p attenuates colon cancer cell migration and invasion.

Applications

Unspecified application

Species

Unspecified reactive species

Anwar Algaber,Raed Madhi,Avin Hawez,Carl-Fredrik Rönnow,Milladur Rahman

Cancer 127:2039-2048 PubMed33764527

2021

Screening and identification of a novel FHL2 mutation by whole exome sequencing in twins with familial Waldenström macroglobulinemia.

Applications

Unspecified application

Species

Unspecified reactive species

Yike Wan,Yuexin Cheng,Yabin Liu,Lijing Shen,Jian Hou

Cancer cell international 20:370 PubMed32774164

2020

LncRNA DLX6-AS1 aggravates the development of ovarian cancer via modulating FHL2 by sponging miR-195-5p.

Applications

Unspecified application

Species

Unspecified reactive species

Lijun Kong,Chengyan Zhang

Cell death & disease 10:372 PubMed31068580

2019

miR-340-FHL2 axis inhibits cell growth and metastasis in ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zheng Huang,Qiuxia Li,Kaili Luo,Qinkai Zhang,Jingwen Geng,Xunzhu Zhou,Yesha Xu,Mengyao Qian,Jian-An Zhang,Liying Ji,Jianmin Wu

Gene 669:99-106 PubMed29800735

2018

Increased expression of FHL2 promotes tumorigenesis in cervical cancer and is correlated with poor prognosis.

Applications

IHC-P, WB

Species

Human, Human

Xuejing Jin,Xinlin Jiao,Jun Jiao,Teng Zhang,Baoxia Cui

Oncology letters 12:1095-1100 PubMed27446400

2016

Plumbagin exhibits an anti-proliferative effect in human osteosarcoma cells by downregulating FHL2 and interfering with Wnt/β-catenin signalling.

Applications

WB

Species

Human

Yuan-Liang Xue,Xiang-Qi Meng,Long-Jun Ma,Zhen Yuan

Basic research in cardiology 109:451 PubMed25358972

2014

FHL2 expression and variants in hypertrophic cardiomyopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Felix W Friedrich,Silke Reischmann,Aileen Schwalm,Andreas Unger,Deepak Ramanujam,Julia Münch,Oliver J Müller,Christian Hengstenberg,Enrique Galve,Philippe Charron,Wolfgang A Linke,Stefan Engelhardt,Monica Patten,Pascale Richard,Jolanda van der Velden,Thomas Eschenhagen,Richard Isnard,Lucie Carrier

Breast cancer research and treatment 127:375-84 PubMed20602165

2010

Dual targeting of EphA2 and ER restores tamoxifen sensitivity in ER/EphA2-positive breast cancer.

Applications

WB

Species

Human

Yesim Gökmen-Polar,Rachel A Toroni,Barbara A Hocevar,Sunil Badve,Qianqian Zhao,Changyu Shen,Elizabeth Bruckheimer,Michael S Kinch,Kathy D Miller
View all publications
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