Rabbit Polyclonal FHL2 antibody. Suitable for ICC/IF, IP, WB, IHC-P and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human FHL2.
View Alternative Names
DRAL, SLIM3, FHL2, Four and a half LIM domains protein 2, FHL-2, LIM domain protein DRAL, Skeletal muscle LIM-protein 3, SLIM-3
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FHL2 antibody (AB12328)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling FHL2 with ab12328 at 1/2000 (0.5 μg/ml). Detection : DAB.
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FHL2 antibody (AB12328)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling FHL2 with ab12328 at 1/1000 (1µg/ml). Detection : DAB.
- ICC/IF
Unknown
Immunocytochemistry/ Immunofluorescence - Anti-FHL2 antibody (AB12328)
ICC image of ab12328 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12328, 1μg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
- IP
Supplier Data
Immunoprecipitation - Anti-FHL2 antibody (AB12328)
FHL2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab12328 at 3 μg/mg lysate (different LOT numbers). Western blot was performed from the immunoprecipitate using ab12328 at 1 μg/ml.
All lanes:
Immunoprecipitation - Anti-FHL2 antibody (ab12328)
Predicted band size: 32 kDa
false
Exposure time: 30s
- WB
Supplier Data
Western blot - Anti-FHL2 antibody (AB12328)
All lanes:
Western blot - Anti-FHL2 antibody (ab12328) at 0.1 µg/mL
Lane 1:
HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 50 µg
Lane 2:
TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate at 50 µg
Lane 3:
NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 50 µg
Predicted band size: 32 kDa
false
Exposure time: 30s
Alternative Products
Primary Antibodies
AB202584
Anti-FHL2 antibody [EPR17860-20]
20ul selling size|RabMAb|Recombinant|KO Validated
![Immunocytochemistry/ Immunofluorescence - Anti-FHL2 antibody [EPR17860-20] (AB202584)](https://content.abcam.com/products/images/fhl2-antibody-epr17860-20-ab202584--immunocytochemistry-immunofluorescence-img86484.jpg)
- 0
- 0
![Immunocytochemistry/ Immunofluorescence - Anti-FHL2 antibody [EPR17860-23] (AB202586)](https://content.abcam.com/products/images/fhl2-antibody-epr17860-23-ab202586--immunocytochemistry-immunofluorescence-img14277.jpg)
Complementary Products
Primary Antibodies
AB32572
Anti-beta Catenin antibody [E247] - ChIP Grade
BOND RX™ Validated|Advanced Validation|RabMAb|Recombinant|KO Validated|Lab Essentials|20ul selling size
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody [E247] - ChIP Grade (AB32572)](https://content.abcam.com/products/images/beta-catenin-antibody-e247-chip-grade-ab32572--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img93749.jpg)
- 5
- 75
Primary Antibodies
AB9485
Anti-GAPDH antibody - Loading Control
BOND RX™ Validated|Lab Essentials
- 5
- 88
Primary Antibodies
AB8227
Anti-beta Actin antibody - Loading Control
BOND RX™ Validated|Lab Essentials
- 5
- 104
Reactivity data
Properties and storage information
Form
Purification technique
Purification notes
Storage buffer
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The FHL2 protein serves critical functions in muscle development and differentiation. It operates as part of a multiprotein complex that interacts with cytoskeletal components contributing importantly to muscle cell adhesion and communication. Moreover FHL2 engages in transcriptional regulation and acts as a coactivator or corepressor of specific transcription factors influencing gene expression patterns relevant to muscle function and maintenance.
Pathways
The FHL2 protein plays significant roles in Wnt and MAPK signaling pathways. In the Wnt signaling pathway FHL2 regulates the transcriptional activity of beta-catenin an essential component of the pathway involved in cell growth and differentiation. Additionally FHL2 contributes to the MAPK signaling pathway by influencing interactions between upstream regulators and downstream targets modulating cellular responses to external stimuli. Through these pathways FHL2 interacts with various proteins including GATA4 and TEF-1 which are vital for its regulatory functions.
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
Target data
Publications (2)
Recent publications for all applications. Explore the full list and refine your search
International journal of experimental pathology 104:64-75 PubMed36694990
2023
Applications
Unspecified application
Species
Unspecified reactive species
The Journal of experimental medicine 218: PubMed34076685
2021
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com