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Rabbit Polyclonal FHL2 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Human Four and a half LIM domains protein 2 aa 50 to C-terminus.


Images

Western blot - Anti-FHL2 antibody (AB137477), expandable thumbnail
  • Western blot - Anti-FHL2 antibody (AB137477), expandable thumbnail
  • Western blot - Anti-FHL2 antibody (AB137477), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FHL2 antibody (AB137477), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human Four and a half LIM domains protein 2 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q14192

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWB
Human
Tested
Tested
Mouse
Expected
Tested
Rat
Predicted
Predicted
Cow
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

1/100.00000 - 1/1000.00000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Rat, Cow

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/500.00000 - 1/3000.00000

Notes

-

Species

Human

Dilution info

1/500.00000 - 1/3000.00000

Notes

-

Predicted
Predicted

Species

Rat, Cow

Dilution info

-

Notes

-

Associated Products

Select an associated product type

5 products for Alternative Product

Target data

Function

May function as a molecular transmitter linking various signaling pathways to transcriptional regulation. Negatively regulates the transcriptional repressor E4F1 and may function in cell growth. Inhibits the transcriptional activity of FOXO1 and its apoptotic function by enhancing the interaction of FOXO1 with SIRT1 and FOXO1 deacetylation. Negatively regulates the calcineurin/NFAT signaling pathway in cardiomyocytes (PubMed:28717008).

Alternative names

Recommended products

Rabbit Polyclonal FHL2 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Human Four and a half LIM domains protein 2 aa 50 to C-terminus.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human Four and a half LIM domains protein 2 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q14192
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The FHL2 protein also known as Four and a Half LIM Domains Protein 2 plays an important role in cellular mechanics. Its molecular mass is approximately 32 kDa. FHL2 is expressed in various tissues with significant presence in cardiac and skeletal muscles as well as the liver. It is involved in the regulation of gene expression by binding to other proteins and DNA through its LIM domains. This ability allows FHL2 to influence cellular structures and functions integrally.

Biological function summary

The FHL2 protein serves critical functions in muscle development and differentiation. It operates as part of a multiprotein complex that interacts with cytoskeletal components contributing importantly to muscle cell adhesion and communication. Moreover FHL2 engages in transcriptional regulation and acts as a coactivator or corepressor of specific transcription factors influencing gene expression patterns relevant to muscle function and maintenance.

Pathways

The FHL2 protein plays significant roles in Wnt and MAPK signaling pathways. In the Wnt signaling pathway FHL2 regulates the transcriptional activity of beta-catenin an essential component of the pathway involved in cell growth and differentiation. Additionally FHL2 contributes to the MAPK signaling pathway by influencing interactions between upstream regulators and downstream targets modulating cellular responses to external stimuli. Through these pathways FHL2 interacts with various proteins including GATA4 and TEF-1 which are vital for its regulatory functions.

Associated diseases and disorders

FHL2 has associations with certain cancers like prostate cancer and cardiomyopathy. In prostate cancer FHL2 overexpression leads to increased tumor progression and is often linked to androgen receptors promoting malignant transformation. In cardiomyopathy alterations in FHL2 expression affect heart muscle integrity and function sometimes in conjunction with interactions involving desmin an important structural protein. Understanding FHL2's connections to these diseases offers insights into potential therapeutic targets.

Product promise

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4 product images

  • Western blot - Anti-FHL2 antibody (ab137477), expandable thumbnail
    This image is courtesy of an anonymous customer review

    Western blot - Anti-FHL2 antibody (ab137477)

    All lanes: Western blot - Anti-FHL2 antibody (ab137477) at 1/1500 dilution

    All lanes: Pig aortic valve interstitial cells lysate at 2.5 µg

    Secondary

    All lanes: HRP-conjugated Goat anti-rabbit IgG (H+L) polyclonal at 1/4500 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 32 kDa

    Observed band size: 32 kDa

    Exposure time: 5min

  • Western blot - Anti-FHL2 antibody (ab137477), expandable thumbnail

    Western blot - Anti-FHL2 antibody (ab137477)

    12% SDS PAGE

    All lanes: Western blot - Anti-FHL2 antibody (ab137477) at 1/1000 dilution

    All lanes: HeLa whole cell lysate at 30 µg

    Predicted band size: 24 kDa, 32 kDa, 43 kDa

  • Western blot - Anti-FHL2 antibody (ab137477), expandable thumbnail

    Western blot - Anti-FHL2 antibody (ab137477)

    12% SDS PAGE

    All lanes: Western blot - Anti-FHL2 antibody (ab137477) at 1/1000 dilution

    All lanes: mouse brain whole cell lysate at 50 µg

    Predicted band size: 32 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FHL2 antibody (ab137477), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FHL2 antibody (ab137477)

    Immunohistochemical analysis of paraffin embedded DLD1
    xenograft labelling FHL2 with ab137477 at a 1/100 dilution.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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