Rabbit Polyclonal FHL2 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Human Four and a half LIM domains protein 2 aa 50 to C-terminus.
IgG
Rabbit
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Liquid
Polyclonal
IHC-P | WB | |
---|---|---|
Human | Tested | Tested |
Mouse | Expected | Tested |
Rat | Predicted | Predicted |
Cow | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Cow | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Cow | Dilution info - | Notes - |
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May function as a molecular transmitter linking various signaling pathways to transcriptional regulation. Negatively regulates the transcriptional repressor E4F1 and may function in cell growth. Inhibits the transcriptional activity of FOXO1 and its apoptotic function by enhancing the interaction of FOXO1 with SIRT1 and FOXO1 deacetylation. Negatively regulates the calcineurin/NFAT signaling pathway in cardiomyocytes (PubMed:28717008).
DRAL, SLIM3, FHL2, DRAL, SLIM3, Four and a half LIM domains protein 2, FHL-2, LIM domain protein DRAL, Skeletal muscle LIM-protein 3, SLIM-3
Rabbit Polyclonal FHL2 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Human Four and a half LIM domains protein 2 aa 50 to C-terminus.
IgG
Rabbit
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
The FHL2 protein also known as Four and a Half LIM Domains Protein 2 plays an important role in cellular mechanics. Its molecular mass is approximately 32 kDa. FHL2 is expressed in various tissues with significant presence in cardiac and skeletal muscles as well as the liver. It is involved in the regulation of gene expression by binding to other proteins and DNA through its LIM domains. This ability allows FHL2 to influence cellular structures and functions integrally.
The FHL2 protein serves critical functions in muscle development and differentiation. It operates as part of a multiprotein complex that interacts with cytoskeletal components contributing importantly to muscle cell adhesion and communication. Moreover FHL2 engages in transcriptional regulation and acts as a coactivator or corepressor of specific transcription factors influencing gene expression patterns relevant to muscle function and maintenance.
The FHL2 protein plays significant roles in Wnt and MAPK signaling pathways. In the Wnt signaling pathway FHL2 regulates the transcriptional activity of beta-catenin an essential component of the pathway involved in cell growth and differentiation. Additionally FHL2 contributes to the MAPK signaling pathway by influencing interactions between upstream regulators and downstream targets modulating cellular responses to external stimuli. Through these pathways FHL2 interacts with various proteins including GATA4 and TEF-1 which are vital for its regulatory functions.
FHL2 has associations with certain cancers like prostate cancer and cardiomyopathy. In prostate cancer FHL2 overexpression leads to increased tumor progression and is often linked to androgen receptors promoting malignant transformation. In cardiomyopathy alterations in FHL2 expression affect heart muscle integrity and function sometimes in conjunction with interactions involving desmin an important structural protein. Understanding FHL2's connections to these diseases offers insights into potential therapeutic targets.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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All lanes: Western blot - Anti-FHL2 antibody (ab137477) at 1/1500 dilution
All lanes: Pig aortic valve interstitial cells lysate at 2.5 µg
All lanes: HRP-conjugated Goat anti-rabbit IgG (H+L) polyclonal at 1/4500 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 32 kDa
Observed band size: 32 kDa
Exposure time: 5min
12% SDS PAGE
All lanes: Western blot - Anti-FHL2 antibody (ab137477) at 1/1000 dilution
All lanes: HeLa whole cell lysate at 30 µg
Predicted band size: 24 kDa, 32 kDa, 43 kDa
12% SDS PAGE
All lanes: Western blot - Anti-FHL2 antibody (ab137477) at 1/1000 dilution
All lanes: mouse brain whole cell lysate at 50 µg
Predicted band size: 32 kDa
Immunohistochemical analysis of paraffin embedded DLD1
xenograft labelling FHL2 with ab137477 at a 1/100 dilution.
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