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AB137477

Anti-FHL2 antibody

5

(2 Reviews)

|

(1 Publication)

Rabbit Polyclonal FHL2 antibody. Suitable for IHC-P, WB and reacts with Mouse, Zebrafish samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human FHL2 aa 50 to C-terminus.

View Alternative Names

DRAL, SLIM3, FHL2, Four and a half LIM domains protein 2, FHL-2, LIM domain protein DRAL, Skeletal muscle LIM-protein 3, SLIM-3

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FHL2 antibody (AB137477)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FHL2 antibody (AB137477)

Immunohistochemical analysis of paraffin-embedded mouse muscle tissue stained for FHL2 protein at cytosol and nucleus with ab137477 at a 1/500 dilution.

Antigen retrieval : Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western blot - Anti-FHL2 antibody (AB137477)
  • WB

Supplier Data

Western blot - Anti-FHL2 antibody (AB137477)

Sample was separated by 12% SDS-PAGE

All lanes:

Western blot - Anti-FHL2 antibody (ab137477)

All lanes:

Mouse heart tissue lysate at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 32 kDa

false

Western blot - Anti-FHL2 antibody (AB137477)
  • WB

Supplier Data

Western blot - Anti-FHL2 antibody (AB137477)

Samples were separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-FHL2 antibody (ab137477) at 1/1000 dilution

All lanes:

Zebrafish heart lysate at 30 µg

false

Western blot - Anti-FHL2 antibody (AB137477)
  • WB

AbReview37211****

Western blot - Anti-FHL2 antibody (AB137477)

All lanes:

Western blot - Anti-FHL2 antibody (ab137477) at 1/1500 dilution

All lanes:

Pig aortic valve interstitial cells lysate at 2.5 µg

Secondary

All lanes:

HRP-conjugated Goat anti-rabbit IgG (H+L) polyclonal at 1/4500 dilution

Predicted band size: 32 kDa

Observed band size: 32 kDa

true

Exposure time: 5min

This image is courtesy of an anonymous Abreview

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human FHL2 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q14192

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebrafish": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The FHL2 protein also known as Four and a Half LIM Domains Protein 2 plays an important role in cellular mechanics. Its molecular mass is approximately 32 kDa. FHL2 is expressed in various tissues with significant presence in cardiac and skeletal muscles as well as the liver. It is involved in the regulation of gene expression by binding to other proteins and DNA through its LIM domains. This ability allows FHL2 to influence cellular structures and functions integrally.
Biological function summary

The FHL2 protein serves critical functions in muscle development and differentiation. It operates as part of a multiprotein complex that interacts with cytoskeletal components contributing importantly to muscle cell adhesion and communication. Moreover FHL2 engages in transcriptional regulation and acts as a coactivator or corepressor of specific transcription factors influencing gene expression patterns relevant to muscle function and maintenance.

Pathways

The FHL2 protein plays significant roles in Wnt and MAPK signaling pathways. In the Wnt signaling pathway FHL2 regulates the transcriptional activity of beta-catenin an essential component of the pathway involved in cell growth and differentiation. Additionally FHL2 contributes to the MAPK signaling pathway by influencing interactions between upstream regulators and downstream targets modulating cellular responses to external stimuli. Through these pathways FHL2 interacts with various proteins including GATA4 and TEF-1 which are vital for its regulatory functions.

FHL2 has associations with certain cancers like prostate cancer and cardiomyopathy. In prostate cancer FHL2 overexpression leads to increased tumor progression and is often linked to androgen receptors promoting malignant transformation. In cardiomyopathy alterations in FHL2 expression affect heart muscle integrity and function sometimes in conjunction with interactions involving desmin an important structural protein. Understanding FHL2's connections to these diseases offers insights into potential therapeutic targets.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May function as a molecular transmitter linking various signaling pathways to transcriptional regulation. Negatively regulates the transcriptional repressor E4F1 and may function in cell growth. Inhibits the transcriptional activity of FOXO1 and its apoptotic function by enhancing the interaction of FOXO1 with SIRT1 and FOXO1 deacetylation. Negatively regulates the calcineurin/NFAT signaling pathway in cardiomyocytes (PubMed : 28717008).
See full target information FHL2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular physiology 233:6578-6588 PubMed29150931

2018

MicroRNA-497 accelerates apoptosis while inhibiting proliferation, migration, and invasion through negative regulation of the MAPK/ERK signaling pathway via RAF-1.

Applications

Unspecified application

Species

Unspecified reactive species

Ling Tao,Chun-Yu Zhang,Lei Guo,Xia Li,Na-Na Han,Qi Zhou,Zhi-Le Liu
View all publications
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