Rabbit Recombinant Monoclonal FHL2 antibody. Carrier free. Suitable for IP, WB, ICC/IF and reacts with Mouse, Rat, Human samples.
Images
![Western blot - Anti-FHL2 antibody [EPR17860-20] - BSA and Azide free (AB251378), expandable thumbnail](https://content.abcam.com/products/images/fhl2-antibody-epr17860-20-bsa-and-azide-free-ab251378--western-blot-img139614.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-FHL2 antibody [EPR17860-20] - BSA and Azide free (AB251378)")
![Western blot - Anti-FHL2 antibody [EPR17860-20] - BSA and Azide free (AB251378), expandable thumbnail](https://content.abcam.com/products/images/fhl2-antibody-epr17860-20-bsa-and-azide-free-ab251378--western-blot-img139797.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-FHL2 antibody [EPR17860-20] - BSA and Azide free (AB251378)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | ICC/IF | |
|---|---|---|---|
| Human | Expected | Tested | Expected |
| Mouse | Expected | Predicted | Expected |
| Rat | Expected | Predicted | Expected |
IP
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
May function as a molecular transmitter linking various signaling pathways to transcriptional regulation. Negatively regulates the transcriptional repressor E4F1 and may function in cell growth. Inhibits the transcriptional activity of FOXO1 and its apoptotic function by enhancing the interaction of FOXO1 with SIRT1 and FOXO1 deacetylation. Negatively regulates the calcineurin/NFAT signaling pathway in cardiomyocytes (PubMed:28717008).
Alternative names
DRAL, SLIM3, FHL2, Four and a half LIM domains protein 2, FHL-2, LIM domain protein DRAL, Skeletal muscle LIM-protein 3, SLIM-3
Recommended products
Rabbit Recombinant Monoclonal FHL2 antibody. Carrier free. Suitable for IP, WB, ICC/IF and reacts with Mouse, Rat, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR17860-20
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab251378 is the carrier-free version of Anti-FHL2 antibody [EPR17860-20] ab202584.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The FHL2 protein also known as Four and a Half LIM Domains Protein 2 plays an important role in cellular mechanics. Its molecular mass is approximately 32 kDa. FHL2 is expressed in various tissues with significant presence in cardiac and skeletal muscles as well as the liver. It is involved in the regulation of gene expression by binding to other proteins and DNA through its LIM domains. This ability allows FHL2 to influence cellular structures and functions integrally.
- Biological function summary
The FHL2 protein serves critical functions in muscle development and differentiation. It operates as part of a multiprotein complex that interacts with cytoskeletal components contributing importantly to muscle cell adhesion and communication. Moreover FHL2 engages in transcriptional regulation and acts as a coactivator or corepressor of specific transcription factors influencing gene expression patterns relevant to muscle function and maintenance.
- Pathways
The FHL2 protein plays significant roles in Wnt and MAPK signaling pathways. In the Wnt signaling pathway FHL2 regulates the transcriptional activity of beta-catenin an essential component of the pathway involved in cell growth and differentiation. Additionally FHL2 contributes to the MAPK signaling pathway by influencing interactions between upstream regulators and downstream targets modulating cellular responses to external stimuli. Through these pathways FHL2 interacts with various proteins including GATA4 and TEF-1 which are vital for its regulatory functions.
- Associated diseases and disorders
FHL2 has associations with certain cancers like prostate cancer and cardiomyopathy. In prostate cancer FHL2 overexpression leads to increased tumor progression and is often linked to androgen receptors promoting malignant transformation. In cardiomyopathy alterations in FHL2 expression affect heart muscle integrity and function sometimes in conjunction with interactions involving desmin an important structural protein. Understanding FHL2's connections to these diseases offers insights into potential therapeutic targets.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-FHL2 antibody [EPR17860-20] - BSA and Azide free (ab251378), expandable thumbnail](https://content.abcam.com/products/images/fhl2-antibody-epr17860-20-bsa-and-azide-free-ab251378--western-blot-img139614.jpg "Western blot - Anti-FHL2 antibody [EPR17860-20] - BSA and Azide free (ab251378)")
Western blot - Anti-FHL2 antibody [EPR17860-20] - BSA and Azide free (ab251378)
This data was developed using the same antibody clone in a different buffer formulation (Anti-FHL2 antibody [EPR17860-20] ab202584).
Lanes 1-3: Merged signal (red and green). Green - Anti-FHL2 antibody [EPR17860-20] ab202584 observed at 32 kDa. Red - loading control Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 observed at 50 kDa.
Anti-FHL2 antibody [EPR17860-20] ab202584 Recombinant Anti-FHL2 antibody [EPR17860-20] was shown to specifically react with FHL2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human FHL2 knockout HeLa cell line ab265475 (knockout cell lysate Human FHL2 knockout HeLa cell lysate ab257441) was used. Wild-type and FHL2 knockout samples were subjected to SDS-PAGE. Anti-FHL2 antibody [EPR17860-20] ab202584 and Anti-alpha Tubulin antibody [DM1A] - Loading Control?(Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) were incubated overnight at 4^°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-FHL2 antibody [EPR17860-20] (Anti-FHL2 antibody [EPR17860-20] ab202584) at 1/1000 dilution
Lane 1: Wild-type HeLa lysate at 20 µg
Lane 2: FHL2 knockout HeLa lysate at 20 µg
Lane 2: Western blot - Human FHL2 knockout HeLa cell line (Human FHL2 knockout HeLa cell line ab265475)
Lane 3: K562 lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 32 kDa
![Western blot - Anti-FHL2 antibody [EPR17860-20] - BSA and Azide free (ab251378), expandable thumbnail](https://content.abcam.com/products/images/fhl2-antibody-epr17860-20-bsa-and-azide-free-ab251378--western-blot-img139797.jpg "Western blot - Anti-FHL2 antibody [EPR17860-20] - BSA and Azide free (ab251378)")
Western blot - Anti-FHL2 antibody [EPR17860-20] - BSA and Azide free (ab251378)
This data was developed using the same antibody clone in a different buffer formulation (Anti-FHL2 antibody [EPR17860-20] ab202584).
Lanes 1 - 3: Merged signal (red and green). Green - Anti-FHL2 antibody [EPR17860-20] ab202584 observed at 32 kDa. Red - loading control Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti-Alpha Tubulin [DM1A] observed at 55kDa.
Anti-FHL2 antibody [EPR17860-20] ab202584 was shown to react with FHL2 in wild-type U-2 OS cells in western blot with loss of signal observed in FHL2 knockout sample. Wild-type and FHL2 knockout U-2 OS cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with Anti-FHL2 antibody [EPR17860-20] ab202584 and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti-Alpha Tubulin [DM1A] overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-FHL2 antibody [EPR17860-20] (Anti-FHL2 antibody [EPR17860-20] ab202584) at 1/1000 dilution
Lane 1: Wild-type U-2 OS cell lysate at 40 µg
Lane 2: FHL2 knockout U-2 OS cell lysate at 40 µg
Lane 2: Western blot - Human FHL2 knockout U-2 OS cell line (Human FHL2 knockout U-2 OS cell line ab262496)
Lane 3: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 40 µg
Performed under reducing conditions.
Predicted band size: 32 kDa
Observed band size: 32 kDa
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