AB180509

Anti-FHL3 antibody [EPR14243(B)]

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal FHL3 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Rat samples. Cited in 1 publication.

View Alternative Names

SLIM2, FHL3, Four and a half LIM domains protein 3, FHL-3, Skeletal muscle LIM-protein 2, SLIM-2

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FHL3 antibody [EPR14243(B)] (AB180509)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FHL3 antibody [EPR14243(B)] (AB180509)

Immunohistochemical analysis of paraffin-embedded Human muscle tissue labeling FHL3 with ab180509 at 1/500 dilution. Counter stain : Hematoxylin

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-FHL3 antibody [EPR14243(B)] (AB180509)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-FHL3 antibody [EPR14243(B)] (AB180509)

Immunofluorescence analysis of A673 cells labeling FHL3 with ab180509 at 1/100 dilution. Secondary ab : Goat anti rabbit IgG (Alexa Fluor® 555) at 1/250 dilution. Fixative : 4% paraformaldehyde. Counter stain : Dapi

Supplier Data

Western blot - Anti-FHL3 antibody [EPR14243(B)] (AB180509)

Blocking buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-FHL3 antibody [EPR14243(B)] (ab180509) at 1/5000 dilution

All lanes:

Human skeletal muscle lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG HRP (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab136636'>ab136636</a>) at 1/500 dilution

Predicted band size: 31 kDa

false

Supplier Data

Western blot - Anti-FHL3 antibody [EPR14243(B)] (AB180509)

Blocking buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-FHL3 antibody [EPR14243(B)] (ab180509) at 1/1000 dilution

Lane 1:

K562 cell lysate at 20 µg

Lane 2:

Rat muscle tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 31 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14243(B)

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FHL3 also known as Four and a Half LIM Domains Protein 3 functions as a transcriptional cofactor. It weighs approximately 30 kDa. FHL3 is expressed in skeletal muscle cardiac muscle and to some degree in other tissues such as the spleen and lung. This protein consists of LIM domains known for mediating important protein-protein interactions facilitating its role in cellular activities.

Biological function summary

The target plays an important role in transcriptional regulation and muscle development. FHL3 serves as part of transcriptional complexes and has been shown to interact with muscle-specific transcription factors. It promotes the correct expression of genes involved in the growth and maintenance of muscle tissues. Through these interactions FHL3 influences the structural and functional integrity of muscle cells.

Pathways

FHL3 operates within the Wnt signaling and the insulin-like growth factor (IGF) pathways. In these pathways it interacts with several proteins such as transcription factors like MyoD and Myogenin which are critical for muscle differentiation and regeneration. These interactions help in transmitting signals that modulate gene expression patterns essential for muscle function and repair.

Mutations or aberrant expression of FHL3 associates with muscular dystrophy and certain heart conditions. In muscular dystrophy alterations in FHL3 affect muscle fiber integrity often through its interactions with other myotonic dystrophy proteins. In cardiac conditions FHL3 involvement impacts the heart muscle structure and function through its connection with proteins involved in cardiac remodeling and stress response such as the protein titin.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Recruited by SOX15 to FOXK1 promoters where it acts as a transcriptional coactivator of FOXK1.

See full target information FHL3

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Neural regeneration research 19:1618-1624 PubMed38051907

2023

Melatonin improves synapse development by PI3K/Akt signaling in a mouse model of autism spectrum disorder.

Applications

Unspecified application

Species

Unspecified reactive species

Luyi Wang,Man Xu,Yan Wang,Feifei Wang,Jing Deng,Xiaoya Wang,Yu Zhao,Ailing Liao,Feng Yang,Shali Wang,Yingbo Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FHL3 antibody [EPR14243(B)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FHL3 antibody [EPR14243(B)] (AB180509)

Immunocytochemistry/ Immunofluorescence - Anti-FHL3 antibody [EPR14243(B)] (AB180509)

Western blot - Anti-FHL3 antibody [EPR14243(B)] (AB180509)

Western blot - Anti-FHL3 antibody [EPR14243(B)] (AB180509)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Proteins & Peptides

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise