AB206692

Anti-FHOD1 antibody

Name: Anti-FHOD1 antibody (ab206692) | Abcam
Brand: Abcam Limited
SKU: AB206692

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(2 Publications )

Rabbit Polyclonal FHOD1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human FHOD1 aa 1100 to C-terminus.

View Alternative Names

FHOS, FHOS1, FHOD1, FH1/FH2 domain-containing protein 1, Formin homolog overexpressed in spleen 1, Formin homology 2 domain-containing protein 1

2 Images

Supplier Data

Immunoprecipitation - Anti-FHOD1 antibody (AB206692)

Detection of FHOD1 in immunoprecipitates of 293T whole cell lysates prepared using NETN lysis buffer (1 mg for IP, 20% of IP loaded) using ab206692 at 6 μg/mg lysate for IP (Lane

and at 1 μg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection : Chemiluminescence with an exposure time of 3 minutes.

All lanes:

Immunoprecipitation - Anti-FHOD1 antibody (ab206692)

Predicted band size: 127 kDa

false

Supplier Data

Western blot - Anti-FHOD1 antibody (AB206692)

Lysates prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-FHOD1 antibody (ab206692) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Predicted band size: 127 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human FHOD1 aa 1100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9Y613

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Cat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Sheep": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab206692 was affinity purified using an epitope specific to FHOD1 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The formin homology domain-containing protein 1 (FHOD1) also known as Formin Homology 2 Domain-containing protein 1 acts as an important regulator of actin dynamics facilitating the polymerization of actin filaments. FHOD1 has a molecular mass of approximately 133 kDa. It is widely expressed in a variety of tissues including the heart lungs and skeletal muscle highlighting its importance across multiple organ systems. FHOD1's ability to interact with actin directly affects cell shape and motility which are critical for various cellular functions.

Biological function summary

FHOD1 plays an essential role in the modulation of the cytoskeleton. It functions as part of the formin family which are known to act as actin nucleators directing the elongation of actin filaments. FHOD1 forms filamentous actin structures and collaborates with other actin-binding proteins. This activity allows FHOD1 to maintain cellular architecture and support processes like cell division and migration.

Pathways

FHOD1 integrates its activity into the regulation of the actin cytoskeleton and Rho GTPase pathways. These pathways influence key cellular processes such as morphogenesis adhesion and motility. FHOD1's interaction with RhoA modulates actin filament assembly enabling it to partner with other proteins like mDia1 and ROCK to orchestrate cytoskeletal arrangements necessary for cell movement and structure integrity.

FHOD1 has connections to cardiovascular and cancer-related pathologies. Its role in maintaining actin filament structure affects cellular dynamics with abnormal expression or function being implicated in heart disease and tumor metastasis. Additionally dysregulation of FHOD1 alongside proteins such as RhoA and mDia1 can contribute to aberrant cellular proliferation and invasion typically observed in malignancies. Understanding FHOD1 interactions might offer potential therapeutic angles to address these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Required for the assembly of F-actin structures, such as stress fibers. Depends on the Rho-ROCK cascade for its activity. Contributes to the coordination of microtubules with actin fibers and plays a role in cell elongation. Acts synergistically with ROCK1 to promote SRC-dependent non-apoptotic plasma membrane blebbing.

See full target information FHOD1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

CNS neuroscience & therapeutics 29:3351-3363 PubMed37211949

2023

FHOD1 is upregulated in glioma cells and attenuates ferroptosis of glioma cells by targeting HSPB1 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Fan Zhang,Lixiang Wu,Songshan Feng,Zijin Zhao,Kui Zhang,Abhimanyu Thakur,Zhijie Xu,Qiuju Liang,Yuanhong Liu,Wei Liu,Yuanliang Yan

Science advances 7:eabj8156 PubMed34860542

2021

MON-2, a Golgi protein, mediates autophagy-dependent longevity in .

Applications

Unspecified application

Species

Unspecified reactive species

Yoonji Jung,Murat Artan,Nari Kim,Jeonghun Yeom,Ara B Hwang,Dae-Eun Jeong,Özlem Altintas,Keunhee Seo,Mihwa Seo,Dongyeop Lee,Wooseon Hwang,Yujin Lee,Jooyeon Sohn,Eun Ji E Kim,Sungeun Ju,Seong Kyu Han,Hyun-Jun Nam,Linnea Adams,Youngjae Ryu,Dong Jin Moon,Chanhee Kang,Joo-Yeon Yoo,Sang Ki Park,Chang Man Ha,Malene Hansen,Sanguk Kim,Cheolju Lee,Seung-Yeol Park,Seung-Jae V Lee

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FHOD1 antibody

Immunoprecipitation - Anti-FHOD1 antibody (AB206692)

Western blot - Anti-FHOD1 antibody (AB206692)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise