Rabbit Polyclonal FHOD1 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human FHOD1 aa 450-600.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human FHOD1 aa 450-600. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y613
Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Required for the assembly of F-actin structures, such as stress fibers. Depends on the Rho-ROCK cascade for its activity. Contributes to the coordination of microtubules with actin fibers and plays a role in cell elongation. Acts synergistically with ROCK1 to promote SRC-dependent non-apoptotic plasma membrane blebbing.
Alternative names
FHOS, FHOS1, FHOD1, FH1/FH2 domain-containing protein 1, Formin homolog overexpressed in spleen 1, Formin homology 2 domain-containing protein 1
Recommended products
Rabbit Polyclonal FHOD1 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human FHOD1 aa 450-600.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human FHOD1 aa 450-600. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y613
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The formin homology domain-containing protein 1 (FHOD1) also known as Formin Homology 2 Domain-containing protein 1 acts as an important regulator of actin dynamics facilitating the polymerization of actin filaments. FHOD1 has a molecular mass of approximately 133 kDa. It is widely expressed in a variety of tissues including the heart lungs and skeletal muscle highlighting its importance across multiple organ systems. FHOD1's ability to interact with actin directly affects cell shape and motility which are critical for various cellular functions.
- Biological function summary
FHOD1 plays an essential role in the modulation of the cytoskeleton. It functions as part of the formin family which are known to act as actin nucleators directing the elongation of actin filaments. FHOD1 forms filamentous actin structures and collaborates with other actin-binding proteins. This activity allows FHOD1 to maintain cellular architecture and support processes like cell division and migration.
- Pathways
FHOD1 integrates its activity into the regulation of the actin cytoskeleton and Rho GTPase pathways. These pathways influence key cellular processes such as morphogenesis adhesion and motility. FHOD1's interaction with RhoA modulates actin filament assembly enabling it to partner with other proteins like mDia1 and ROCK to orchestrate cytoskeletal arrangements necessary for cell movement and structure integrity.
- Associated diseases and disorders
FHOD1 has connections to cardiovascular and cancer-related pathologies. Its role in maintaining actin filament structure affects cellular dynamics with abnormal expression or function being implicated in heart disease and tumor metastasis. Additionally dysregulation of FHOD1 alongside proteins such as RhoA and mDia1 can contribute to aberrant cellular proliferation and invasion typically observed in malignancies. Understanding FHOD1 interactions might offer potential therapeutic angles to address these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunocytochemistry/ Immunofluorescence - Anti-FHOD1 antibody (ab224461)
PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for FHOD1 (green) using ab224461 at 4 μg/ml in ICC/IF.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FHOD1 antibody (ab224461)
Paraffin-embedded human lymph node tissue stained for FHOD1 using ab224461 at 1/200 dilution in immunohistochemical analysis.
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