Mouse Polyclonal FHOD1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human FHOD1.
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- Oncology letters 22:7122021FHOD1 is upregulated in gastric cancer and promotes the proliferation and invasion of gastric cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChengfei Jiang,Binbin Yuan,Bo Hang,Jian-Hua Mao,Xiaoping Zou,Pin WangPubMed 34457067
- Platelets :1-122020Loss of mDia1 and Fhod1 impacts platelet formation but not platelet function.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMalou Zuidscherwoude et. al.PubMed 32981398
- The Biochemical journal 476:2953-29632019Active FHOD1 promotes the formation of functional actin stress fibers.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXuemeng Shi,Shuangshuang Zhao,Jinping Cai,Gary Wong,Yaming JiuPubMed 31657439
- Scientific reports 7:12532017Lamins and nesprin-1 mediate inside-out mechanical coupling in muscle cell precursors through FHOD1.Applications:WBReactive species:Unspecified reactive speciesChristine Schwartz et. al.PubMed 28455503
- Anatomical record (Hoboken, N.J. : 2007) 297:1560-702014The formin FHOD1 in cardiomyocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJoseph Dwyer,Marlene Pluess,Thomas Iskratsch,Cristobal G Dos Remedios,Elisabeth EhlerPubMed 25125170
- The Journal of biological chemistry 288:35437-512013Interactome analysis reveals ezrin can adopt multiple conformational states.
- Journal of immunology (Baltimore, Md. : 1950) 188:4496-5052012SIRPα/CD172a and FHOD1 are unique markers of littoral cells, a recently evolved major cell population of red pulp of human spleen.
Key facts
- Isotype
- IgG
- Host species
- Mouse
- Storage buffer
pH: 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human FHOD1. Database link Q9Y613
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/1000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 13 µg/mL | Notes - |
Associated Products
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3 products for Alternative Product
Target data
Function
Required for the assembly of F-actin structures, such as stress fibers. Depends on the Rho-ROCK cascade for its activity. Contributes to the coordination of microtubules with actin fibers and plays a role in cell elongation. Acts synergistically with ROCK1 to promote SRC-dependent non-apoptotic plasma membrane blebbing.
Alternative names
FHOS, FHOS1, FHOD1, FH1/FH2 domain-containing protein 1, Formin homolog overexpressed in spleen 1, Formin homology 2 domain-containing protein 1
Recommended products
Mouse Polyclonal FHOD1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human FHOD1.
Key facts
- Isotype
- IgG
- Host species
- Mouse
- Storage buffer
pH: 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human FHOD1. Database link Q9Y613
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The formin homology domain-containing protein 1 (FHOD1) also known as Formin Homology 2 Domain-containing protein 1 acts as an important regulator of actin dynamics facilitating the polymerization of actin filaments. FHOD1 has a molecular mass of approximately 133 kDa. It is widely expressed in a variety of tissues including the heart lungs and skeletal muscle highlighting its importance across multiple organ systems. FHOD1's ability to interact with actin directly affects cell shape and motility which are critical for various cellular functions.
- Biological function summary
FHOD1 plays an essential role in the modulation of the cytoskeleton. It functions as part of the formin family which are known to act as actin nucleators directing the elongation of actin filaments. FHOD1 forms filamentous actin structures and collaborates with other actin-binding proteins. This activity allows FHOD1 to maintain cellular architecture and support processes like cell division and migration.
- Pathways
FHOD1 integrates its activity into the regulation of the actin cytoskeleton and Rho GTPase pathways. These pathways influence key cellular processes such as morphogenesis adhesion and motility. FHOD1's interaction with RhoA modulates actin filament assembly enabling it to partner with other proteins like mDia1 and ROCK to orchestrate cytoskeletal arrangements necessary for cell movement and structure integrity.
- Associated diseases and disorders
FHOD1 has connections to cardiovascular and cancer-related pathologies. Its role in maintaining actin filament structure affects cellular dynamics with abnormal expression or function being implicated in heart disease and tumor metastasis. Additionally dysregulation of FHOD1 alongside proteins such as RhoA and mDia1 can contribute to aberrant cellular proliferation and invasion typically observed in malignancies. Understanding FHOD1 interactions might offer potential therapeutic angles to address these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Western blot - Anti-FHOD1 antibody (ab73443)
All lanes: Western blot - Anti-FHOD1 antibody (ab73443) at 1/500 dilution
Lane 1: FHOD1 transfected 293T cell lysate at 25 µg
Lane 2: non-transfected lysate at 25 µg
SecondaryAll lanes: goat anti-mouse IgG(H&L)-HRP conjugate at 1/2500 dilution
Predicted band size: 127 kDa
Observed band size: 145 kDa, 80 kDa
Western blot - Anti-FHOD1 antibody (ab73443)
All lanes: Western blot - Anti-FHOD1 antibody (ab73443) at 1/500 dilution
All lanes: K-562 cell lysate at 25 µg
SecondaryAll lanes: goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution
Predicted band size: 127 kDa
Observed band size: 150 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FHOD1 antibody (ab73443)
Immunoperoxidase staining of formalin-fixed paraffin-embedded human spleen using ab73443, at 3 ug/ml of purified version of the antibody.
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-FHOD1 antibody (ab73443)
FHOD1 western blot using anti-FHOD1 antibody ab73443. Publication image and figure legend from Schwartz, C., Fischer, M., et al., 2017, Sci Rep, PubMed 28455503.
ab73443 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab73443 please see the product overview.
FHOD1 expression in myoblasts on soft matrix (12 kPa). (A) Confocal images of WT, Nespr-1ΔKASH and LMNAΔK32 myoblasts on soft matrix and stained for F-actin (phalloidin, red) and FHOD1 (green). Nuclei are stained with DAPI (blue). (B) Representative western-blot of FHOD1 in WT, Nespr-1ΔKASH and LMNAΔK32 myoblasts cultured on soft substrates. Histogram represents FHOD1 quantifications obtained in WT, Nespr-1ΔKASH and LMNAΔK32 myoblasts and normalized by GAPDH. Values are means ± SEM, n = 6 in WT, Nespr-1ΔKASH and LMNAΔK32 myoblasts; *p < 0.05. Only significant difference is figured; au: arbitrary units. (C) Histogram represent mRNA concentrations of FHOD1, DIAPH1 and DIAPH3 normalized to β2 microglobulin expression and expressed in arbitrary units (au). Values are means ± SEM, n = 5 in WT, Nespr-1ΔKASH and LMNAΔK32 myoblasts, ***p < 0.001 compared with WT. Only significant difference is figured.
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