Anti-FHOD1 antibody

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FHOD1 antibody (AB73443)

Immunoperoxidase staining of formalin-fixed paraffin-embedded human spleen using ab73443, at 3 ug/ml of purified version of the antibody.

• WB

Unknown

Western blot - Anti-FHOD1 antibody (AB73443)

All lanes:

Western blot - Anti-FHOD1 antibody (ab73443) at 1/500 dilution

Lane 1:

FHOD1 transfected 293T cell lysate at 25 µg

Lane 2:

non-transfected lysate at 25 µg

Secondary

All lanes:

goat anti-mouse IgG(H&L)-HRP conjugate at 1/2500 dilution

Predicted band size: 127 kDa

Observed band size: 145 kDa,80 kDa

false

• WB

Unknown

Western blot - Anti-FHOD1 antibody (AB73443)

All lanes:

Western blot - Anti-FHOD1 antibody (ab73443) at 1/500 dilution

All lanes:

K-562 cell lysate at 25 µg

Secondary

All lanes:

goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

Predicted band size: 127 kDa

Observed band size: 150 kDa

false

• WB

CiteAb

Western blot - Anti-FHOD1 antibody (AB73443)

FHOD1 western blot using anti-FHOD1 antibody ab73443. Publication image and figure legend from Schwartz, C., Fischer, M., et al., 2017, Sci Rep, PubMed 28455503.

ab73443 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab73443 please see the product overview.

FHOD1 expression in myoblasts on soft matrix (12 kPa). (A) Confocal images of WT, Nespr-1ΔKASH and LMNAΔK32 myoblasts on soft matrix and stained for F-actin (phalloidin, red) and FHOD1 (green). Nuclei are stained with DAPI (blue). (B) Representative western-blot of FHOD1 in WT, Nespr-1ΔKASH and LMNAΔK32 myoblasts cultured on soft substrates. Histogram represents FHOD1 quantifications obtained in WT, Nespr-1ΔKASH and LMNAΔK32 myoblasts and normalized by GAPDH. Values are means ± SEM, n = 6 in WT, Nespr-1ΔKASH and LMNAΔK32 myoblasts; *p < 0.05. Only significant difference is figured; au : arbitrary units. (C) Histogram represent mRNA concentrations of FHOD1, DIAPH1 and DIAPH3 normalized to β2 microglobulin expression and expressed in arbitrary units (au). Values are means ± SEM, n = 5 in WT, Nespr-1ΔKASH and LMNAΔK32 myoblasts, ***p < 0.001 compared with WT. Only significant difference is figured.

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