JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB157589

Anti-FHOD1 (phospho T1141) antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Polyclonal FHOD1 phospho T1141 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human FHOD1 phospho T1141 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

FHOS, FHOS1, FHOD1, FH1/FH2 domain-containing protein 1, Formin homolog overexpressed in spleen 1, Formin homology 2 domain-containing protein 1

1 Images
Western blot - Anti-FHOD1 (phospho T1141) antibody (AB157589)
  • WB

Unknown

Western blot - Anti-FHOD1 (phospho T1141) antibody (AB157589)

All lanes:

Western blot - Anti-FHOD1 (phospho T1141) antibody (ab157589) at 1/1000 dilution

Lane 1:

K562 cells stimulated with calyculin A (100 nM) for 30 min

Lane 2:

K562 cells stimulated with calyculin A (100 nM) for 30 min, then treated with lambda phosphatase

Predicted band size: 127 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human FHOD1 phospho T1141 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q9Y613

Specificity

The immunogen sequence is highly conserved in rat and mouse FHOD1, and is well conserved in FHOD3 (Thr-1399).

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab157589 was cross-adsorbed to unphosphorylated FHOD1 (Thr-1141)peptide before affinity purification using phospho-FHOD1 (Thr-1141) peptide (without carrier).
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The formin homology domain-containing protein 1 (FHOD1) also known as Formin Homology 2 Domain-containing protein 1 acts as an important regulator of actin dynamics facilitating the polymerization of actin filaments. FHOD1 has a molecular mass of approximately 133 kDa. It is widely expressed in a variety of tissues including the heart lungs and skeletal muscle highlighting its importance across multiple organ systems. FHOD1's ability to interact with actin directly affects cell shape and motility which are critical for various cellular functions.
Biological function summary

FHOD1 plays an essential role in the modulation of the cytoskeleton. It functions as part of the formin family which are known to act as actin nucleators directing the elongation of actin filaments. FHOD1 forms filamentous actin structures and collaborates with other actin-binding proteins. This activity allows FHOD1 to maintain cellular architecture and support processes like cell division and migration.

Pathways

FHOD1 integrates its activity into the regulation of the actin cytoskeleton and Rho GTPase pathways. These pathways influence key cellular processes such as morphogenesis adhesion and motility. FHOD1's interaction with RhoA modulates actin filament assembly enabling it to partner with other proteins like mDia1 and ROCK to orchestrate cytoskeletal arrangements necessary for cell movement and structure integrity.

FHOD1 has connections to cardiovascular and cancer-related pathologies. Its role in maintaining actin filament structure affects cellular dynamics with abnormal expression or function being implicated in heart disease and tumor metastasis. Additionally dysregulation of FHOD1 alongside proteins such as RhoA and mDia1 can contribute to aberrant cellular proliferation and invasion typically observed in malignancies. Understanding FHOD1 interactions might offer potential therapeutic angles to address these conditions.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Required for the assembly of F-actin structures, such as stress fibers. Depends on the Rho-ROCK cascade for its activity. Contributes to the coordination of microtubules with actin fibers and plays a role in cell elongation. Acts synergistically with ROCK1 to promote SRC-dependent non-apoptotic plasma membrane blebbing.
See full target information FHOD1 phospho T1141
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular systems biology 13:951 PubMed29101300

2017

Pharmacoproteomic characterisation of human colon and rectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Martin Frejno,Riccardo Zenezini Chiozzi,Mathias Wilhelm,Heiner Koch,Runsheng Zheng,Susan Klaeger,Benjamin Ruprecht,Chen Meng,Karl Kramer,Anna Jarzab,Stephanie Heinzlmeir,Elaine Johnstone,Enric Domingo,David Kerr,Moritz Jesinghaus,Julia Slotta-Huspenina,Wilko Weichert,Stefan Knapp,Stephan M Feller,Bernhard Kuster
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB224461

Anti-FHOD1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FHOD1 antibody (AB224461)

  • 5
  • 1
Primary Antibodies

AB73443

Anti-FHOD1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FHOD1 antibody (AB73443)

  • 0
  • 0
Primary Antibodies

AB206692

Anti-FHOD1 antibody

Western blot - Anti-FHOD1 antibody (AB206692)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB67721

Anti-ABLIM3 antibody

Western blot - Anti-ABLIM3 antibody (AB67721)

  • 4
  • 1
Primary Antibodies

AB245660

Anti-DIAPH3 antibody

Western blot - Anti-DIAPH3 antibody (AB245660)

  • 5
  • 5
Primary Antibodies

AB171088

Anti-TCR beta F1 antibody [8A3]

Flow Cytometry - Anti-TCR beta F1 antibody [8A3] (AB171088)

  • 5
  • 1
Primary Antibodies

AB172509

Anti-CIKS/ACT1 antibody

Western blot - Anti-CIKS/ACT1 antibody (AB172509)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com