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AB224215

Anti-FIAT antibody

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(1 Publication)

Rabbit Polyclonal FIAT antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human TXLNG aa 50 to C-terminus.

View Alternative Names

CXorf15, ELRG, LSR5, TXLNG, Gamma-taxilin, Environmental lipopolysaccharide-responding gene protein, Factor inhibiting ATF4-mediated transcription, Lipopolysaccharide-specific response protein 5, FIAT

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FIAT antibody (AB224215)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FIAT antibody (AB224215)

Paraffin-embedded human bone marrow tissue stained for FIAT using ab224215 at 1/500 dilution in immunohistochemical analysis.

Western blot - Anti-FIAT antibody (AB224215)
  • WB

Supplier Data

Western blot - Anti-FIAT antibody (AB224215)

All lanes:

Western blot - Anti-FIAT antibody (ab224215) at 1/250 dilution

Lane 1:

RT4 (human urinary bladder cancer cell line) cell lysate

Lane 2:

EFO-21 cell lysate

Lane 3:

A431 (human epidermoid carcinoma cell line) cell lysate

Predicted band size: 60 kDa

true

Western blot - Anti-FIAT antibody (AB224215)
  • WB

Supplier Data

Western blot - Anti-FIAT antibody (AB224215)

All lanes:

Western blot - Anti-FIAT antibody (ab224215) at 1/250 dilution

Lane 1:

NIH/3T3 (mouse embryonic fibroblast cell line) cell lysate

Lane 2:

NBT-II (rat Wistar bladder tumor cell line) cell lysate

Predicted band size: 60 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human TXLNG aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9NUQ3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FIAT also known as Factor Inhibiting ATF4-mediated Transcription is a transcriptional regulator with a molecular weight of approximately 37 kDa. You can find it expressed in various tissues but it is especially prevalent in bone tissue. Mechanically FIAT interacts with ATF4 inhibiting its transcriptional activity. FIAT binds to the bZip region of ATF4 preventing ATF4 from regulating genes that are critical for osteoblast differentiation and function.
Biological function summary

FIAT plays an important role in bone development and homeostasis. It is not known to be part of a large protein complex but it functions by altering transcriptional outputs. FIAT limits ATF4's ability to activate osteoblast-specific target genes consequently influencing bone formation and remodeling. By doing this FIAT helps to maintain proper bone density and strength balancing the activities of osteoblasts and osteoclasts.

Pathways

Research indicates that FIAT is involved in the osteoblastic bone formation pathway. It regulates this pathway through its interaction with ATF4 which is an important player in translating extracellular signals to gene expression responses. FIAT also intersects with the BMP (Bone Morphogenetic Protein) pathway influencing BMP target genes and interacting with other transcription factors like Runx2.

FIAT links to conditions affecting bone health such as osteoporosis. Due to its inhibition of ATF4 FIAT indirectly contributes to osteoporosis by reducing osteoblast activity leading to decreased bone density. FIAT's relationship with ATF4 connects it to the regulation of genes altered in osteoporotic bones. Research is ongoing to explore the therapeutic potential of modulating FIAT activity aiming to mitigate the effects of osteoporosis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May be involved in intracellular vesicle traffic. Inhibits ATF4-mediated transcription, possibly by dimerizing with ATF4 to form inactive dimers that cannot bind DNA. May be involved in regulating bone mass density through an ATF4-dependent pathway. May be involved in cell cycle progression.
See full target information TXLNG
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of neural transmission (Vienna, Austria : 1996) : PubMed40886229

2025

The effect of recombinant botulinum neurotoxin A on neuropathic pain in the spared nerve injury mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Rasmus E Hammer,Akinyemi A Omoniyi,Mette Richner,Stephane Lezmi,Christian B Vaegter,Mikhail Kalinichev,Pall Karlsson,Jens R Nyengaard
View all publications
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Product promise

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