AB218846

Anti-Fibrillarin antibody [4A4]

(2 Reviews)

(2 Publications)

Mouse Monoclonal Fibrillarin antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human FBL.

View Alternative Names

FIB1, FLRN, FBL, rRNA 2'-O-methyltransferase fibrillarin, 34 kDa nucleolar scleroderma antigen, Histone-glutamine methyltransferase, U6 snRNA 2'-O-methyltransferase fibrillarin

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin antibody [4A4] (AB218846)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin antibody [4A4] (AB218846)

Immunofluorescent analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Fibrillarin with ab218846 at 1/1000 dilution (red). The cells were counterstained with a chicken antibody to vimentin at 1/5000 dilution (green) and DAPI to label the nuclei (blue).

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin antibody [4A4] (AB218846)

C6 (rat glial tumor cell line) (Panel A) and HEK-293 (human epithelial cell line from embryonic kidney) (Panel B) cells stained for Fibrillarin (red) using ab218846 at 1/1000 dilution in ICC/IF. Co-stained with chicken pAb to vimentin, at 1/10000 dilution (green). The blue is DAPI staining of nuclear DNA.

Supplier Data

Western blot - Anti-Fibrillarin antibody [4A4] (AB218846)

Fibrillarin detection is shown in green.

Also stained with a rabbit antibody to UCHL-1 at 1/3000 dilution (red).

All lanes:

Western blot - Anti-Fibrillarin antibody [4A4] (ab218846) at 1/2000 dilution

Lane 1:

MW ladder

Lane 2:

Rat whole brain lysate

Lane 3:

Mouse whole brain lysate

Lane 4:

NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate

Lane 5:

HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate

Lane 6:

HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate

Lane 7:

SH-SY5Y (Human neuroblastoma cell line from bone marrow) cell lysate

Predicted band size: 33 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

4A4

Isotype

IgG1

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human FBL.

P22087

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/5000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000 - 1/2000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/5000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/5000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000 - 1/2000", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

Preservative: 0.03% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins (PubMed : 24352239, PubMed : 30540930, PubMed : 32017898). Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA (PubMed : 30540930). Site specificity is provided by a guide RNA that base pairs with the substrate (By similarity). Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA (By similarity). Probably catalyzes 2'-O-methylation of U6 snRNAs in box C/D RNP complexes (PubMed : 32017898). U6 snRNA 2'-O-methylation is required for mRNA splicing fidelity (PubMed : 32017898). Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ104me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus (PubMed : 24352239, PubMed : 30540930). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed : 34516797).

See full target information FBL

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Aging 15:13176-13193 PubMed37988198

2023

Investigating EGF and PAG1 as necroptosis-related biomarkers for diabetic nephropathy: an and validation study.

Applications

Unspecified application

Species

Unspecified reactive species

Yuejun Wang,Linlin Zhang,Zhiping Peng

Parasitology 148:341-353 PubMed33100232

2020

infections induce G cell cycle arrest and a senescence-like phenotype in endothelial host cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zahady D Velásquez,Sara López-Osorio,Daniel Waiger,Carolina Manosalva,Learta Pervizaj-Oruqaj,Susanne Herold,Carlos Hermosilla,Anja Taubert

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Fibrillarin antibody [4A4]

Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin antibody [4A4] (AB218846)

Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin antibody [4A4] (AB218846)

Western blot - Anti-Fibrillarin antibody [4A4] (AB218846)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (2)

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