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Mouse Monoclonal Fibrillarin antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human FBL.


Images

Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin antibody [4A4] (AB218846), expandable thumbnail
  • Western blot - Anti-Fibrillarin antibody [4A4] (AB218846), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin antibody [4A4] (AB218846), expandable thumbnail

Publications

Key facts

Isotype

IgG1

Host species

Mouse

Storage buffer

Preservative: 0.03% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Human FBL. Database link P22087

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IF
Human
Tested
Tested
Mouse
Tested
Expected
Rat
Tested
Tested

Tested
Tested

Species

Mouse

Dilution info

1/2000.00000 - 1/5000.00000

Notes

-

Species

Rat

Dilution info

1/2000.00000 - 1/5000.00000

Notes

-

Species

Human

Dilution info

1/2000.00000 - 1/5000.00000

Notes

-

Tested
Tested

Species

Rat

Dilution info

1/1000.00000 - 1/2000.00000

Notes

-

Species

Human

Dilution info

1/1000.00000 - 1/2000.00000

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

7 products for Alternative Product

Target data

Function

S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins (PubMed:24352239, PubMed:30540930, PubMed:32017898). Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA (PubMed:30540930). Site specificity is provided by a guide RNA that base pairs with the substrate (By similarity). Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA (By similarity). Probably catalyzes 2'-O-methylation of U6 snRNAs in box C/D RNP complexes (PubMed:32017898). U6 snRNA 2'-O-methylation is required for mRNA splicing fidelity (PubMed:32017898). Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ104me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus (PubMed:24352239, PubMed:30540930). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed:34516797).

Alternative names

Recommended products

Mouse Monoclonal Fibrillarin antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human FBL.

Key facts

Isotype

IgG1

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Recombinant Full Length Protein corresponding to Human FBL. Database link P22087
Clone number

4A4

Purification technique

Affinity purification Protein G

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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3 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin antibody [4A4] (ab218846), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin antibody [4A4] (ab218846)

    C6 (rat glial tumor cell line) (Panel A) and HEK-293 (human epithelial cell line from embryonic kidney) (Panel B) cells stained for Fibrillarin (red) using ab218846 at 1/1000 dilution in ICC/IF. Co-stained with chicken pAb to vimentin, at 1/10000 dilution (green). The blue is DAPI staining of nuclear DNA.

  • Western blot - Anti-Fibrillarin antibody [4A4] (ab218846), expandable thumbnail

    Western blot - Anti-Fibrillarin antibody [4A4] (ab218846)

    Fibrillarin detection is shown in green.

    Also stained with a rabbit antibody to UCHL-1 at 1/3000 dilution (red).

    All lanes: Western blot - Anti-Fibrillarin antibody [4A4] (ab218846) at 1/2000 dilution

    Lane 1: MW ladder

    Lane 2: Rat whole brain lysate

    Lane 3: Mouse whole brain lysate

    Lane 4: NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate

    Lane 5: HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate

    Lane 6: HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate

    Lane 7: SH-SY5Y (Human neuroblastoma cell line from bone marrow) cell lysate

    Predicted band size: 33 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin antibody [4A4] (ab218846), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin antibody [4A4] (ab218846)

    Immunofluorescent analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Fibrillarin with ab218846 at 1/1000 dilution (red). The cells were counterstained with a chicken antibody to vimentin at 1/5000 dilution (green) and DAPI to label the nuclei (blue).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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