Mouse Monoclonal Fibrillarin antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human FBL.
IgG1
Mouse
Preservative: 0.03% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Liquid
Monoclonal
WB | ICC/IF | |
---|---|---|
Human | Tested | Tested |
Mouse | Tested | Expected |
Rat | Tested | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/2000.00000 - 1/5000.00000 | Notes - |
Species Rat | Dilution info 1/2000.00000 - 1/5000.00000 | Notes - |
Species Human | Dilution info 1/2000.00000 - 1/5000.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info 1/1000.00000 - 1/2000.00000 | Notes - |
Species Human | Dilution info 1/1000.00000 - 1/2000.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Select an associated product type
S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins (PubMed:24352239, PubMed:30540930, PubMed:32017898). Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA (PubMed:30540930). Site specificity is provided by a guide RNA that base pairs with the substrate (By similarity). Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA (By similarity). Probably catalyzes 2'-O-methylation of U6 snRNAs in box C/D RNP complexes (PubMed:32017898). U6 snRNA 2'-O-methylation is required for mRNA splicing fidelity (PubMed:32017898). Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ104me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus (PubMed:24352239, PubMed:30540930). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed:34516797).
FIB1, FLRN, FIB1, FBL, FLRN, rRNA 2'-O-methyltransferase fibrillarin, 34 kDa nucleolar scleroderma antigen, Histone-glutamine methyltransferase, U6 snRNA 2'-O-methyltransferase fibrillarin
Mouse Monoclonal Fibrillarin antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human FBL.
IgG1
Mouse
Preservative: 0.03% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Liquid
Monoclonal
4A4
Affinity purification Protein G
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
C6 (rat glial tumor cell line) (Panel A) and HEK-293 (human epithelial cell line from embryonic kidney) (Panel B) cells stained for Fibrillarin (red) using ab218846 at 1/1000 dilution in ICC/IF. Co-stained with chicken pAb to vimentin, at 1/10000 dilution (green). The blue is DAPI staining of nuclear DNA.
Fibrillarin detection is shown in green.
Also stained with a rabbit antibody to UCHL-1 at 1/3000 dilution (red).
All lanes: Western blot - Anti-Fibrillarin antibody [4A4] (ab218846) at 1/2000 dilution
Lane 1: MW ladder
Lane 2: Rat whole brain lysate
Lane 3: Mouse whole brain lysate
Lane 4: NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate
Lane 5: HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate
Lane 6: HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 7: SH-SY5Y (Human neuroblastoma cell line from bone marrow) cell lysate
Predicted band size: 33 kDa
Immunofluorescent analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Fibrillarin with ab218846 at 1/1000 dilution (red). The cells were counterstained with a chicken antibody to vimentin at 1/5000 dilution (green) and DAPI to label the nuclei (blue).
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com