AB249113

Anti-Fibrillarin + Fibrillarin-like protein 1 antibody [EPR10822(B)] - BSA and Azide free

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal Fibrillarin antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

FIB1, FLRN, FBL, rRNA 2'-O-methyltransferase fibrillarin, 34 kDa nucleolar scleroderma antigen, Histone-glutamine methyltransferase, U6 snRNA 2'-O-methyltransferase fibrillarin, RNA 2'-O-methyltransferase FBLL1, Fibrillarin like 1

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin + Fibrillarin-like protein 1 antibody [EPR10822(B)] - BSA and Azide free (AB249113)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin + Fibrillarin-like protein 1 antibody [EPR10822(B)] - BSA and Azide free (AB249113)

This data was developed using ab154806, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of HepG2 cells labeling Fibrillarin with ab154806 at 1/250 dilution.

Unknown

Western blot - Anti-Fibrillarin + Fibrillarin-like protein 1 antibody [EPR10822(B)] - BSA and Azide free (AB249113)

This data was developed using ab154806, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Fibrillarin + Fibrillarin-like protein 1 antibody [EPR10822(B)] - Nucleolar Marker (<a href='/en-us/products/primary-antibodies/fibrillarin-antibody-epr10822b-nucleolar-marker-ab154806'>ab154806</a>) at 1/1000 dilution

Lane 1:

HeLa lysate at 10 µg

Lane 2:

HepG2 lysate at 10 µg

Lane 3:

293T lysate at 10 µg

Lane 4:

Molt4 lysate at 10 µg

Predicted band size: 33 kDa

false

Unconjugated

Anti-Fibrillarin + Fibrillarin-like protein 1 antibody [EPR10822(B)] - Nucleolar Marker

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10822(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

ab249113 is the carrier-free version of ab154806.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins (PubMed : 24352239, PubMed : 30540930, PubMed : 32017898). Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA (PubMed : 30540930). Site specificity is provided by a guide RNA that base pairs with the substrate (By similarity). Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA (By similarity). Probably catalyzes 2'-O-methylation of U6 snRNAs in box C/D RNP complexes (PubMed : 32017898). U6 snRNA 2'-O-methylation is required for mRNA splicing fidelity (PubMed : 32017898). Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ104me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus (PubMed : 24352239, PubMed : 30540930). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed : 34516797).

See full target information FBL

Additional targets

FBLL1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Developmental cell 60:2744-2760.e9 PubMed40460835

2025

ATG9A and ARFIP2 cooperate to control PI4P levels for lysosomal repair.

Applications

Unspecified application

Species

Unspecified reactive species

Stefano De Tito,Eugenia Almacellas,Daniel Dai Yu,Emily Millard,Wenxin Zhang,Cecilia de Heus,Christophe Queval,Javier H Hervás,Enrica Pellegrino,Ioanna Panagi,Ditte Fogde,Teresa L M Thurston,Judith Klumperman,Maximiliano Gutierrez,Sharon A Tooze

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Fibrillarin + Fibrillarin-like protein 1 antibody [EPR10822(B)] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin + Fibrillarin-like protein 1 antibody [EPR10822(B)] - BSA and Azide free (AB249113)

Western blot - Anti-Fibrillarin + Fibrillarin-like protein 1 antibody [EPR10822(B)] - BSA and Azide free (AB249113)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Additional targets

Publications (1)

Product promise