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AB154806

Anti-Fibrillarin + Fibrillarin-like protein 1 antibody [EPR10822(B)] - Nucleolar Marker

5

(1 Review)

|

(6 Publications)

Rabbit Recombinant Monoclonal Fibrillarin antibody. Nucleolus marker. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 6 publications.

View Alternative Names

FIB1, FLRN, FBL, rRNA 2'-O-methyltransferase fibrillarin, 34 kDa nucleolar scleroderma antigen, Histone-glutamine methyltransferase, U6 snRNA 2'-O-methyltransferase fibrillarin

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin + Fibrillarin-like protein 1 antibody [EPR10822(B)] - Nucleolar Marker (AB154806)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin + Fibrillarin-like protein 1 antibody [EPR10822(B)] - Nucleolar Marker (AB154806)

Immunofluorescence analysis of HepG2 cells labeling Fibrillarin with ab154806 at 1/250 dilution.

Western blot - Anti-Fibrillarin + Fibrillarin-like protein 1 antibody [EPR10822(B)] - Nucleolar Marker (AB154806)
  • WB

Unknown

Western blot - Anti-Fibrillarin + Fibrillarin-like protein 1 antibody [EPR10822(B)] - Nucleolar Marker (AB154806)

All lanes:

Western blot - Anti-Fibrillarin + Fibrillarin-like protein 1 antibody [EPR10822(B)] - Nucleolar Marker (ab154806) at 1/1000 dilution

Lane 1:

HeLa lysate at 10 µg

Lane 2:

HepG2 lysate at 10 µg

Lane 3:

293T lysate at 10 µg

Lane 4:

Molt4 lysate at 10 µg

Predicted band size: 33 kDa

false

  • Carrier free

    Anti-Fibrillarin + Fibrillarin-like protein 1 antibody [EPR10822(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10822(B)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins (PubMed : 24352239, PubMed : 30540930, PubMed : 32017898). Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA (PubMed : 30540930). Site specificity is provided by a guide RNA that base pairs with the substrate (By similarity). Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA (By similarity). Probably catalyzes 2'-O-methylation of U6 snRNAs in box C/D RNP complexes (PubMed : 32017898). U6 snRNA 2'-O-methylation is required for mRNA splicing fidelity (PubMed : 32017898). Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ104me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus (PubMed : 24352239, PubMed : 30540930). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed : 34516797).
See full target information FBL

Additional targets

FBLL1
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:6606 PubMed40675952

2025

Somatostatin-expressing interneurons induce early NO-driven and late specific astrocyte-mediated vasodilation.

Applications

Unspecified application

Species

Unspecified reactive species

Thanh Tan Vo,Won Beom Jung,Tong Jin,Geun Ho Im,Soohyun Lee,Seong-Gi Kim

PLoS genetics 19:e1010854 PubMed37639467

2023

rRNA transcription is integral to phase separation and maintenance of nucleolar structure.

Applications

Unspecified application

Species

Unspecified reactive species

Soma Dash,Maureen C Lamb,Jeffrey J Lange,Mary C McKinney,Dai Tsuchiya,Fengli Guo,Xia Zhao,Timothy J Corbin,MaryEllen Kirkman,Kym Delventhal,Emma L Moore,Sean McKinney,Rita Shiang,Paul A Trainor

Molecular oncology 17:27-36 PubMed36370117

2022

Ribosome biogenesis-based predictive biomarkers in endocrine therapy (Anastrozole) combined with mTOR inhibitor (Vistusertib) in endometrial cancer: translational study from the VICTORIA trial in collaboration with the GINECO group.

Applications

Unspecified application

Species

Unspecified reactive species

Nour-El-Houda Mourksi,Cécile Dalban,Amélie Colombe-Vermorel,Laetitia Odeyer,Valentin Simioni,Jean-Sébastien Frenel,Michel Fabbro,Fernando Bazan,Sophie Abadie-Lacourtoisie,Elodie Coquan,Séverine Martinez,Gwenaelle Garin,Séverine Tabone-Eglinger,Isabelle Treilleux,Sylvie Chabaud,David Pérol,Isabelle Ray-Coquard,Pierre-Etienne Heudel,Jean-Jacques Diaz,Virginie Marcel

Bioengineering (Basel, Switzerland) 9: PubMed36004921

2022

Bioinformatic Analysis and In Vitro and In Vivo Experiments Reveal That Fibrillarin Participates in the Promotion of Lung Metastasis in Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Weixin Luo,Shusheng Lin,Yipei Huang,Ke Zhu,Fapeng Zhang,Junlong Lin,Yufei Qin,Ziyu Zhou,Wenrui Wu,Chao Liu

Non-coding RNA research 7:55-65 PubMed35261930

2022

Adaptation of the protein translational apparatus during ATDC5 chondrogenic differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Mandy M F Steinbusch,Guus G H van den Akker,Andy Cremers,Adhiambo M A Witlox,Heleen M Staal,Mandy J Peffers,Lodewijk W van Rhijn,Marjolein M J Caron,Tim J M Welting

Physiological reports 5: PubMed29122955

2017

DNPEP is not the only peptidase that produces SPAK fragments in kidney.

Applications

Unspecified application

Species

Unspecified reactive species

Rainelli Koumangoye,Eric Delpire
View all publications
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