Rabbit Recombinant Monoclonal Fibrillarin antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
IHC-P | WB | ICC/IF | Flow Cyt (Intra) | |
---|---|---|---|---|
Human | Not recommended | Tested | Tested | Tested |
Mouse | Not recommended | Tested | Tested | Expected |
Rat | Not recommended | Tested | Expected | Expected |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes - |
Species Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
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S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins (PubMed:24352239, PubMed:30540930, PubMed:32017898). Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA (PubMed:30540930). Site specificity is provided by a guide RNA that base pairs with the substrate (By similarity). Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA (By similarity). Probably catalyzes 2'-O-methylation of U6 snRNAs in box C/D RNP complexes (PubMed:32017898). U6 snRNA 2'-O-methylation is required for mRNA splicing fidelity (PubMed:32017898). Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ104me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus (PubMed:24352239, PubMed:30540930). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed:34516797).
FIB1, FLRN, FBL, rRNA 2'-O-methyltransferase fibrillarin, 34 kDa nucleolar scleroderma antigen, Histone-glutamine methyltransferase, U6 snRNA 2'-O-methyltransferase fibrillarin
Rabbit Recombinant Monoclonal Fibrillarin antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
Yes
EPR10823(B)
Affinity purification Protein A
Blue Ice
+4°C
+4°C
Do Not Freeze
ab240147 is the carrier-free version of Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker ab166630.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Immunocytochemistry/Immunofluorescence analysis of HT-29 (human colorectal adenocarcinoma) cells labelling Fibrillarin (green) with purified Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker ab166630 at 1/5000. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as a nuclear counterstain.
Secondary Only Control: PBS was used instead of the primary antibody as the negative control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker ab166630).
Immunofluorescent analysis of HeLa cells labeling Fibrillarin with Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker ab166630 at a 1/100 dilution.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker ab166630).
Intracellular flow cytometric analysis of permeabilized MOLT4 cells using Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker ab166630 at a 1/10 dilution (red) or a Rabbit IgG (negative) (green). This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker ab166630).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker ab166630).
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.
All lanes: Western blot - Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker ab166630) at 1/1000 dilution
Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg
Lane 3: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg
Lane 4: PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 33 kDa
Observed band size: 33 kDa
Exposure time: 10s
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker ab166630).
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling Fibrillarin with Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker ab166630 at 1/200 (0.5 μg/ml) dilution followed by secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedused at 1/1000 dilution (2μg/ml) (Green). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 μg/ml) (Red). The Nuclear counterstain was DAPI (Blue).
Confocal image showing nuclear staining in NIH/3T3 cell line.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker ab166630).
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.
All lanes: Western blot - Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker ab166630) at 1/1000 dilution
Lane 1: Mouse liver tissue lysate at 20 µg
Lane 2: Mouse testis tissue lysate at 20 µg
Lane 3: Mouse spleen tissue lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 33 kDa
Observed band size: 33 kDa
Exposure time: 81s
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.
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