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Rabbit Recombinant Monoclonal Fibrillarin antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin antibody [EPR10823(B)] - BSA and Azide free (AB240147), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin antibody [EPR10823(B)] - BSA and Azide free (AB240147), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Fibrillarin antibody [EPR10823(B)] - BSA and Azide free (AB240147), expandable thumbnail
  • Western blot - Anti-Fibrillarin antibody [EPR10823(B)] - BSA and Azide free (AB240147), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin antibody [EPR10823(B)] - BSA and Azide free (AB240147), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IFFlow Cyt (Intra)
Human
Not recommended
Tested
Tested
Tested
Mouse
Not recommended
Tested
Tested
Expected
Rat
Not recommended
Tested
Expected
Expected

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species

Mouse

Dilution info

-

Notes

-

Species

Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human, Mouse

Dilution info

-

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

5 products for Alternative Version

1 product for Alternative Product

Target data

Function

S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins (PubMed:24352239, PubMed:30540930, PubMed:32017898). Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA (PubMed:30540930). Site specificity is provided by a guide RNA that base pairs with the substrate (By similarity). Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA (By similarity). Probably catalyzes 2'-O-methylation of U6 snRNAs in box C/D RNP complexes (PubMed:32017898). U6 snRNA 2'-O-methylation is required for mRNA splicing fidelity (PubMed:32017898). Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ104me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus (PubMed:24352239, PubMed:30540930). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed:34516797).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal Fibrillarin antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR10823(B)

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab240147 is the carrier-free version of Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker ab166630.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin antibody [EPR10823(B)] - BSA and Azide free (ab240147), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin antibody [EPR10823(B)] - BSA and Azide free (ab240147)

    Immunocytochemistry/Immunofluorescence analysis of HT-29 (human colorectal adenocarcinoma) cells labelling Fibrillarin (green) with purified Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker ab166630 at 1/5000. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as a nuclear counterstain.  

    Secondary Only Control: PBS was used instead of the primary antibody as the negative control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker ab166630).

  • Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin antibody [EPR10823(B)] - BSA and Azide free (ab240147), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin antibody [EPR10823(B)] - BSA and Azide free (ab240147)

    Immunofluorescent analysis of HeLa cells labeling Fibrillarin with Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker ab166630 at a 1/100 dilution.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker ab166630).

  • Flow Cytometry (Intracellular) - Anti-Fibrillarin antibody [EPR10823(B)] - BSA and Azide free (ab240147), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Fibrillarin antibody [EPR10823(B)] - BSA and Azide free (ab240147)

    Intracellular flow cytometric analysis of permeabilized MOLT4 cells using Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker ab166630 at a 1/10 dilution (red) or a Rabbit IgG (negative) (green). This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker ab166630).

  • Western blot - Anti-Fibrillarin antibody [EPR10823(B)] - BSA and Azide free (ab240147), expandable thumbnail

    Western blot - Anti-Fibrillarin antibody [EPR10823(B)] - BSA and Azide free (ab240147)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker ab166630).

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.

    All lanes: Western blot - Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker ab166630) at 1/1000 dilution

    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

    Lane 3: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

    Lane 4: PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 33 kDa

    Observed band size: 33 kDa

    Exposure time: 10s

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.

  • Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin antibody [EPR10823(B)] - BSA and Azide free (ab240147), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin antibody [EPR10823(B)] - BSA and Azide free (ab240147)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker ab166630).

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling Fibrillarin with Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker ab166630 at 1/200 (0.5 μg/ml) dilution followed by secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedused at 1/1000 dilution (2μg/ml) (Green). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 μg/ml) (Red). The Nuclear counterstain was DAPI (Blue).

    Confocal image showing nuclear staining in NIH/3T3 cell line.
    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Western blot - Anti-Fibrillarin antibody [EPR10823(B)] - BSA and Azide free (ab240147), expandable thumbnail

    Western blot - Anti-Fibrillarin antibody [EPR10823(B)] - BSA and Azide free (ab240147)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker ab166630).

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.

    All lanes: Western blot - Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker ab166630) at 1/1000 dilution

    Lane 1: Mouse liver tissue lysate at 20 µg

    Lane 2: Mouse testis tissue lysate at 20 µg

    Lane 3: Mouse spleen tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 33 kDa

    Observed band size: 33 kDa

    Exposure time: 81s

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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