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Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab166630) is a rabbit monoclonal antibody that is used to detect Fibrillarin in Western Blot, Flow Cytometry (Intra), Flow Cytometry, ICC/IF. Suitable for Human, Mouse, Rat samples.



- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency

-Over 20 publications


Images

Western blot - Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (AB166630), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (AB166630), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (AB166630), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (AB166630), expandable thumbnail
  • Western blot - Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (AB166630), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IFFlow Cyt (Intra)
Human
Not recommended
Tested
Tested
Tested
Mouse
Not recommended
Tested
Tested
Expected
Rat
Not recommended
Tested
Expected
Expected

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species
Mouse
Dilution info
-
Notes

-

Species
Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/5000
Notes

-

Species
Mouse
Dilution info
1/1000 - 1/5000
Notes

-

Species
Rat
Dilution info
1/1000 - 1/5000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/5000
Notes

For unpurified use at 1/100 - 1/250

Species
Mouse
Dilution info
1/5000
Notes

For unpurified use at 1/100 - 1/250

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/10 - 1/100
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins (PubMed:24352239, PubMed:30540930, PubMed:32017898). Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA (PubMed:30540930). Site specificity is provided by a guide RNA that base pairs with the substrate (By similarity). Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA (By similarity). Probably catalyzes 2'-O-methylation of U6 snRNAs in box C/D RNP complexes (PubMed:32017898). U6 snRNA 2'-O-methylation is required for mRNA splicing fidelity (PubMed:32017898). Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ104me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus (PubMed:24352239, PubMed:30540930). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed:34516797).

Alternative names

Recommended products

Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab166630) is a rabbit monoclonal antibody that is used to detect Fibrillarin in Western Blot, Flow Cytometry (Intra), Flow Cytometry, ICC/IF. Suitable for Human, Mouse, Rat samples.



- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency

-Over 20 publications

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR10823(B)
Purity
Tissue culture supernatant
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

What is this antibody validated in?


Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab166630) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunocytochemistry/immunofluorescence (ICC/IF), in Human, Mouse, Rat samples.

What is the molecular weight of Fibrillarin?


Anti-Fibrillarin [EPR10823(B)] - Nucleolar Marker (ab166630) specifically detects a band for Fibrillarin (UniProt: P22087) at a molecular weight of 33kDa.

Recommended positive controls


293T, HeLa, HepG2, HT-29 and MOLT4 cell lysates; ; HeLa cells. Permeabilized MOLT4 cells; Immunoprecipitation pellet from HepG2 whole cell lysate (ab7900).WB: 293T, HeLa, NIH/3T3 and PC-12 cell lysate; Mouse liver, spleen and testis tissue lysate.ICC/IF: NIH/3T3 cells.

Trusted by the scientific community


Anti-Fibrillarin [EPR10823(B)] - Nucleolar Marker (ab166630) was first used in a scientific publication in 2013 and has been cited over 20 times in peer-reviewed journals.

Trial sizes available!


Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.Other related products


We have a range of other formats of antibody clone [EPR10823(B)] also available for your convenience:
ab166630, Alexa Fluor® 488 - Alexa Fluor® 488 Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker ab184817, HRP - HRP Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker ab196980, Alexa Fluor® 568 - Alexa Fluor® 568 Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker ab202540, Alexa Fluor® 594 - Alexa Fluor® 594 Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker ab203400, Alexa Fluor® 555 - Alexa Fluor® 555 Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker ab203410, Carrier free - Anti-Fibrillarin antibody [EPR10823(B)] - BSA and Azide free ab240147



Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Western blot - Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab166630), expandable thumbnail

    Western blot - Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab166630)

    All lanes: Western blot - Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab166630) at 1/1000 dilution

    Lane 1: 293T cell lysate at 10 µg

    Lane 2: HeLa cell lysate at 10 µg

    Lane 3: HepG2 cell lysate at 10 µg

    Lane 4: MOLT4 cell lysate at 10 µg

    Predicted band size: 33 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab166630), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab166630)

    Immunocytochemistry/Immunofluorescence analysis of HT-29 (human colorectal adenocarcinoma) cells labelling Fibrillarin (green) with purified ab166630 at 1/5000. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as a nuclear counterstain.

    Secondary Only Control: PBS was used instead of the primary antibody as the negative control.

  • Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab166630), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab166630)

    Immunofluorescent analysis of HeLa cells labeling Fibrillarin with ab166630 at a 1/100 dilution.

  • Flow Cytometry (Intracellular) - Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab166630), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab166630)

    Intracellular flow cytometric analysis of permeabilized MOLT4 cells using ab166630 at a 1/10 dilution (red) or a Rabbit IgG (negative) (green).

  • Western blot - Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab166630), expandable thumbnail

    Western blot - Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab166630)

    All lanes: Western blot - Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab166630) at 1/1000 dilution

    All lanes: immunoprecipitation pellet from HepG2 cell lysate at 10 µg

    Predicted band size: 33 kDa

  • Western blot - Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab166630), expandable thumbnail

    Western blot - Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab166630)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.

    All lanes: Western blot - Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab166630) at 1/1000 dilution

    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

    Lane 3: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

    Lane 4: PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 33 kDa

    Observed band size: 33 kDa

    Exposure time: 10s

  • Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab166630), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab166630)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling Fibrillarin with ab166630 at 1/200 (0.5 μg/ml) dilution followed by secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedused at 1/1000 dilution (2μg/ml) (Green). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 μg/ml) (Red). The Nuclear counterstain was DAPI (Blue).

    Confocal image showing nuclear staining in NIH/3T3 cell line.
    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Western blot - Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab166630), expandable thumbnail

    Western blot - Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab166630)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.

    All lanes: Western blot - Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab166630) at 1/1000 dilution

    Lane 1: Mouse liver tissue lysate at 20 µg

    Lane 2: Mouse testis tissue lysate at 20 µg

    Lane 3: Mouse spleen tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 33 kDa

    Observed band size: 33 kDa

    Exposure time: 81s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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