Anti-Fibrillarin+Fibrillarin-like protein 1 antibody [RM1082]

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Fibrillarin+Fibrillarin-like protein 1 antibody [RM1082] (AB314506)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Hela (human cervical adenocarcinoma epithelial cell) cells labelling Fibrillarin with ab314506 at 1/500 dilution (0.1 ug)/Red (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrillarin+Fibrillarin-like protein 1 antibody [RM1082] (AB314506)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Fibrillarin with ab314506 at 1/2000 (0.249 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Nucleolus staining on human tonsil The section was incubated with ab314506 at 4°C overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Heat mediated antigen retrieval was performed using ab93678 (citrate buffer, pH 6.0)

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin+Fibrillarin-like protein 1 antibody [RM1082] (AB314506)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling Fibrillarin with ab314506 at 1/500 (0.994 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green). Confocal image showing nucleolar staining in HeLa cell line.Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.

• IP

Supplier Data

Immunoprecipitation - Anti-Fibrillarin+Fibrillarin-like protein 1 antibody [RM1082] (AB314506)

Fibrillarin was immunoprecipitated from 0.35 mg HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate with ab314506 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab314506 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate Lane 2 : ab314506 IP in HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab314506 in HeLa whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Lower band around 26kDa is supposed to be the degradation form of fibrillarin according to the literature (PMID : 31347059).

All lanes:

Immunoprecipitation - Anti-Fibrillarin+Fibrillarin-like protein 1 antibody [RM1082] (ab314506) at 1/30 dilution

All lanes:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 5s

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin+Fibrillarin-like protein 1 antibody [RM1082] (AB314506)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 (mouse embryonic fibroblast cell) cells labelling Fibrillarin with ab314506 at 1/500 (0.994 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green). Confocal image showing nucleolar staining in NIH/3T3 cell line.Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Fibrillarin+Fibrillarin-like protein 1 antibody [RM1082] (AB314506)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized PC-12 (rat adrenal gland pheochromocytoma cell) cells labelling Fibrillarin with ab314506 at 1/500 dilution (0.1 ug)/Red (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Fibrillarin+Fibrillarin-like protein 1 antibody [RM1082] (AB314506)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling Fibrillarin with ab314506 at 1/500 dilution (0.1 ug)/Red (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrillarin+Fibrillarin-like protein 1 antibody [RM1082] (AB314506)

Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling Fibrillarin with ab314506 at 1/2000 (0.249 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Nucleolus staining on mouse testis. The section was incubated with ab314506 at 4°C overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Heat mediated antigen retrieval was performed using ab93678 (citrate buffer, pH 6.0)

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrillarin+Fibrillarin-like protein 1 antibody [RM1082] (AB314506)

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Fibrillarin with ab314506 at 1/2000 (0.249 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Nucleolus staining on mouse liver. The section was incubated with ab314506 at 4°C overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Heat mediated antigen retrieval was performed using ab93678 (citrate buffer, pH 6.0)

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin+Fibrillarin-like protein 1 antibody [RM1082] (AB314506)

Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized PC-12 (rat adrenal gland pheochromocytoma cell) cells labelling Fibrillarin with ab314506 at 1/500 (0.994 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing nucleolar staining in PC-12 cell line.Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrillarin+Fibrillarin-like protein 1 antibody [RM1082] (AB314506)

Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling Fibrillarin with ab314506 at 1/2000 (0.249 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Nucleolus staining on rat liver. The section was incubated with ab314506 at 4°C overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Heat mediated antigen retrieval was performed using ab93678 (citrate buffer, pH 6.0)

• IP

Supplier Data

Immunoprecipitation - Anti-Fibrillarin+Fibrillarin-like protein 1 antibody [RM1082] (AB314506)

Fibrillarin was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast) whole cell lysate with ab314506 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab314506 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate Lane 2 : ab314506 IP in NIH/3T3 (mouse embryonic fibroblast) whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab314506 in NIH/3T3 whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Lower band around 26kDa is supposed to be the degradation form of fibrillarin according to the literature (PMID : 31347059).

All lanes:

Immunoprecipitation - Anti-Fibrillarin+Fibrillarin-like protein 1 antibody [RM1082] (ab314506) at 1/30 dilution

All lanes:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 5s

• IP

Supplier Data

Immunoprecipitation - Anti-Fibrillarin+Fibrillarin-like protein 1 antibody [RM1082] (AB314506)

Fibrillarin was immunoprecipitated from 0.35 mg PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate with ab314506 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab314506 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate Lane 2 : ab314506 IP in PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab314506 in PC-12 whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Lower band around 26kDa is supposed to be the degradation form of fibrillarin according to the literature (PMID : 31347059).

All lanes:

Immunoprecipitation - Anti-Fibrillarin+Fibrillarin-like protein 1 antibody [RM1082] (ab314506) at 1/30 dilution

All lanes:

PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 5s

• WB

Supplier Data

Western blot - Anti-Fibrillarin+Fibrillarin-like protein 1 antibody [RM1082] (AB314506)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Lysates were freshly made and used for Western blotting immediately to minimize protein degradation. Exposure time : Lanes 1-4 : 26 seconds；Lanes 5 and 6 : 81 seconds；Lanes 7 and 8 : 70 seconds

All lanes:

Western blot - Anti-Fibrillarin+Fibrillarin-like protein 1 antibody [RM1082] (ab314506) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 3:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 4:

PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 20 µg

Lane 5:

Rat testis tissue lysate at 20 µg

Lane 6:

Rat spleen tissue lysate at 20 µg

Lane 7:

Mouse testis tissue lysate at 20 µg

Lane 8:

Mouse spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 33 kDa

false