Rabbit Recombinant Monoclonal Fibrillin 1 antibody. Carrier free. Suitable for IHC-P, Dot, ICC/IF, I-ELISA, Flow Cyt (Intra) and reacts with Human, Recombinant fragment - Human samples.
Images
![Indirect ELISA - Anti-Fibrillin 1 antibody [EPR27362-78] - BSA and Azide free (AB315385), expandable thumbnail](https://content.abcam.com/products/images/fibrillin-1-antibody-epr27362-78-bsa-and-azide-free-ab315385--indirect-elisa-img424748.jpg/_jcr_content/renditions/webp-475.webp "Indirect ELISA - Anti-Fibrillin 1 antibody [EPR27362-78] - BSA and Azide free (AB315385)")
![Dot Blot - Anti-Fibrillin 1 antibody [EPR27362-78] - BSA and Azide free (AB315385), expandable thumbnail](https://content.abcam.com/products/images/fibrillin-1-antibody-epr27362-78-bsa-and-azide-free-ab315385--dot-blot-img424745.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-Fibrillin 1 antibody [EPR27362-78] - BSA and Azide free (AB315385)")
![Flow Cytometry (Intracellular) - Anti-Fibrillin 1 antibody [EPR27362-78] - BSA and Azide free (AB315385), expandable thumbnail](https://content.abcam.com/products/images/fibrillin-1-antibody-epr27362-78-bsa-and-azide-free-ab315385--flow-cytometry-intracellular-img424729.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Fibrillin 1 antibody [EPR27362-78] - BSA and Azide free (AB315385)")
![Flow Cytometry (Intracellular) - Anti-Fibrillin 1 antibody [EPR27362-78] - BSA and Azide free (AB315385), expandable thumbnail](https://content.abcam.com/products/images/fibrillin-1-antibody-epr27362-78-bsa-and-azide-free-ab315385--flow-cytometry-intracellular-img424728.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Fibrillin 1 antibody [EPR27362-78] - BSA and Azide free (AB315385)")
![Immunocytochemistry/ Immunofluorescence - Anti-Fibrillin 1 antibody [EPR27362-78] - BSA and Azide free (AB315385), expandable thumbnail](https://content.abcam.com/products/images/fibrillin-1-antibody-epr27362-78-bsa-and-azide-free-ab315385--immunocytochemistry-immunofluorescence-img424708.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Fibrillin 1 antibody [EPR27362-78] - BSA and Azide free (AB315385)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | Dot | ICC/IF | I-ELISA | Flow Cyt (Intra) | WB | IP | |
|---|---|---|---|---|---|---|---|
| Human | Tested | Expected | Tested | Expected | Tested | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Recombinant fragment - Human | Not recommended | Tested | Not recommended | Tested | Not recommended | Not recommended | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Recombinant fragment - Human | Dilution info - | Notes - |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Recombinant fragment - Human | Dilution info - | Notes - |
I-ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Recombinant fragment - Human | Dilution info - | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Recombinant fragment - Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Recombinant fragment - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
Fibrillin-1. Structural component of the 10-12 nm diameter microfibrils of the extracellular matrix, which conveys both structural and regulatory properties to load-bearing connective tissues (PubMed:15062093, PubMed:1860873). Fibrillin-1-containing microfibrils provide long-term force bearing structural support (PubMed:27026396). In tissues such as the lung, blood vessels and skin, microfibrils form the periphery of the elastic fiber, acting as a scaffold for the deposition of elastin (PubMed:27026396). In addition, microfibrils can occur as elastin-independent networks in tissues such as the ciliary zonule, tendon, cornea and glomerulus where they provide tensile strength and have anchoring roles (PubMed:27026396). Fibrillin-1 also plays a key role in tissue homeostasis through specific interactions with growth factors, such as the bone morphogenetic proteins (BMPs), growth and differentiation factors (GDFs) and latent transforming growth factor-beta-binding proteins (LTBPs), cell-surface integrins and other extracellular matrix protein and proteoglycan components (PubMed:27026396). Regulates osteoblast maturation by controlling TGF-beta bioavailability and calibrating TGF-beta and BMP levels, respectively (By similarity). Negatively regulates osteoclastogenesis by binding and sequestering an osteoclast differentiation and activation factor TNFSF11 (PubMed:24039232). This leads to disruption of TNFSF11-induced Ca(2+) signaling and impairment of TNFSF11-mediated nuclear translocation and activation of transcription factor NFATC1 which regulates genes important for osteoclast differentiation and function (PubMed:24039232). Mediates cell adhesion via its binding to cell surface receptors integrins ITGAV:ITGB3 and ITGA5:ITGB1 (PubMed:12807887, PubMed:17158881). Binds heparin and this interaction has an important role in the assembly of microfibrils (PubMed:11461921). Asprosin. Adipokine secreted by white adipose tissue that plays an important regulatory role in the glucose metabolism of liver, muscle and pancreas (PubMed:27087445, PubMed:30853600). Hormone that targets the liver in response to fasting to increase plasma glucose levels (PubMed:27087445). Binds the olfactory receptor OR4M1 at the surface of hepatocytes and promotes hepatocyte glucose release by activating the protein kinase A activity in the liver, resulting in rapid glucose release into the circulation (PubMed:27087445, PubMed:31230984). May act as a regulator of adaptive thermogenesis by inhibiting browning and energy consumption, while increasing lipid deposition in white adipose tissue (By similarity). Also acts as an orexigenic hormone that increases appetite: crosses the blood brain barrier and exerts effects on the hypothalamus (By similarity). In the arcuate nucleus of the hypothalamus, asprosin directly activates orexigenic AgRP neurons and indirectly inhibits anorexigenic POMC neurons, resulting in appetite stimulation (By similarity). Activates orexigenic AgRP neurons via binding to the olfactory receptor OR4M1 (By similarity). May also play a role in sperm motility in testis via interaction with OR4M1 receptor (By similarity).
Alternative names
FBN, FBN1, Fibrillin-1
Recommended products
Rabbit Recombinant Monoclonal Fibrillin 1 antibody. Carrier free. Suitable for IHC-P, Dot, ICC/IF, I-ELISA, Flow Cyt (Intra) and reacts with Human, Recombinant fragment - Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR27362-78
- Purification technique
- Affinity purification Protein A
- Specificity
This antibody has weak cross reaction with human Fibrillin 3.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab315385 is the carrier-free version of Anti-Fibrillin 1 antibody [EPR27362-78] ab315384.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
10 product images
![Indirect ELISA - Anti-Fibrillin 1 antibody [EPR27362-78] - BSA and Azide free (ab315385), expandable thumbnail](https://content.abcam.com/products/images/fibrillin-1-antibody-epr27362-78-bsa-and-azide-free-ab315385--indirect-elisa-img424748.jpg "Indirect ELISA - Anti-Fibrillin 1 antibody [EPR27362-78] - BSA and Azide free (ab315385)")
Indirect ELISA - Anti-Fibrillin 1 antibody [EPR27362-78] - BSA and Azide free (ab315385)
This data was developed using Anti-Fibrillin 1 antibody [EPR27362-78] ab315384, the same antibody clone in a different buffer formulation.
Indirect ELISA analysis of Anti-Fibrillin 1 antibody [EPR27362-78] ab315384 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1:2500 dilution.
Antigen: Human Fibrillin-1,Human Fibrillin-2,Human Fibrillin-3.
Antigen concentration: 1000 ng/ml
![Dot Blot - Anti-Fibrillin 1 antibody [EPR27362-78] - BSA and Azide free (ab315385), expandable thumbnail](https://content.abcam.com/products/images/fibrillin-1-antibody-epr27362-78-bsa-and-azide-free-ab315385--dot-blot-img424745.jpg "Dot Blot - Anti-Fibrillin 1 antibody [EPR27362-78] - BSA and Azide free (ab315385)")
Dot Blot - Anti-Fibrillin 1 antibody [EPR27362-78] - BSA and Azide free (ab315385)
This data was developed using Anti-Fibrillin 1 antibody [EPR27362-78] ab315384, the same antibody clone in a different buffer formulation.
Dot blot analysis of Fibrillin 1 using Anti-Fibrillin 1 antibody [EPR27362-78] ab315384 at 1:1000 (0.508 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:1000 (0.508 ug/ml) dilution.
Exposure time: 180 seconds.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This antibody has weak cross reaction with human Fibrillin 3.
The recombinant proteins of Fibrillin 1, Fibrillin 2 and Fibrillin 3 were made in house.
![Flow Cytometry (Intracellular) - Anti-Fibrillin 1 antibody [EPR27362-78] - BSA and Azide free (ab315385), expandable thumbnail](https://content.abcam.com/products/images/fibrillin-1-antibody-epr27362-78-bsa-and-azide-free-ab315385--flow-cytometry-intracellular-img424729.jpg "Flow Cytometry (Intracellular) - Anti-Fibrillin 1 antibody [EPR27362-78] - BSA and Azide free (ab315385)")
Flow Cytometry (Intracellular) - Anti-Fibrillin 1 antibody [EPR27362-78] - BSA and Azide free (ab315385)
This data was developed using Anti-Fibrillin 1 antibody [EPR27362-78] ab315384, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized T-47D (human ductal breast epithelial tumor epithelial cell, Left) / ARPE-19 (human eye epithelial cell, Right) cells labelling Fibrillin 1 with Anti-Fibrillin 1 antibody [EPR27362-78] ab315384 at 1/500 dilution (0.1 ug)/Red (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
Negative control: T-47D.
![Flow Cytometry (Intracellular) - Anti-Fibrillin 1 antibody [EPR27362-78] - BSA and Azide free (ab315385), expandable thumbnail](https://content.abcam.com/products/images/fibrillin-1-antibody-epr27362-78-bsa-and-azide-free-ab315385--flow-cytometry-intracellular-img424728.jpg "Flow Cytometry (Intracellular) - Anti-Fibrillin 1 antibody [EPR27362-78] - BSA and Azide free (ab315385)")
Flow Cytometry (Intracellular) - Anti-Fibrillin 1 antibody [EPR27362-78] - BSA and Azide free (ab315385)
This data was developed using Anti-Fibrillin 1 antibody [EPR27362-78] ab315384, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized U-937 (human histiocytic lymphoma monocyte, Left) / A-172 (human brain glioblastoma cell, Right) cells labelling Fibrillin 1 with Anti-Fibrillin 1 antibody [EPR27362-78] ab315384 at 1/500 dilution (0.1 ug)/Red (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
Negative control: U-937.
![Immunocytochemistry/ Immunofluorescence - Anti-Fibrillin 1 antibody [EPR27362-78] - BSA and Azide free (ab315385), expandable thumbnail](https://content.abcam.com/products/images/fibrillin-1-antibody-epr27362-78-bsa-and-azide-free-ab315385--immunocytochemistry-immunofluorescence-img424708.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Fibrillin 1 antibody [EPR27362-78] - BSA and Azide free (ab315385)")
Immunocytochemistry/ Immunofluorescence - Anti-Fibrillin 1 antibody [EPR27362-78] - BSA and Azide free (ab315385)
This data was developed using Anti-Fibrillin 1 antibody [EPR27362-78] ab315384, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized ARPE-19 (human eye epithelial cell) cells labelling Fibrillin 1 with Anti-Fibrillin 1 antibody [EPR27362-78] ab315384 at 1/100 (5.08 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).
Confocal image showing cytoplasmic staining in ARPE-19 cell line. Negative control: T-47D. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.
![Immunocytochemistry/ Immunofluorescence - Anti-Fibrillin 1 antibody [EPR27362-78] - BSA and Azide free (ab315385), expandable thumbnail](https://content.abcam.com/products/images/fibrillin-1-antibody-epr27362-78-bsa-and-azide-free-ab315385--immunocytochemistry-immunofluorescence-img424707.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Fibrillin 1 antibody [EPR27362-78] - BSA and Azide free (ab315385)")
Immunocytochemistry/ Immunofluorescence - Anti-Fibrillin 1 antibody [EPR27362-78] - BSA and Azide free (ab315385)
This data was developed using Anti-Fibrillin 1 antibody [EPR27362-78] ab315384, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized A-172 (human brain glioblastoma cell) cells labelling Fibrillin 1 with Anti-Fibrillin 1 antibody [EPR27362-78] ab315384 at 1/100 (5.08 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).
Confocal image showing cytoplasmic staining in A-172 cell line. Negative control: U-937. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrillin 1 antibody [EPR27362-78] - BSA and Azide free (ab315385), expandable thumbnail](https://content.abcam.com/products/images/fibrillin-1-antibody-epr27362-78-bsa-and-azide-free-ab315385--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img424660.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrillin 1 antibody [EPR27362-78] - BSA and Azide free (ab315385)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrillin 1 antibody [EPR27362-78] - BSA and Azide free (ab315385)
This data was developed using Anti-Fibrillin 1 antibody [EPR27362-78] ab315384, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling Fibrillin 1 with Anti-Fibrillin 1 antibody [EPR27362-78] ab315384 at 1/100 (5.08 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on stromal connective tissue of human colon carcinoma. The section was incubated with Anti-Fibrillin 1 antibody [EPR27362-78] ab315384 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with citrate buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrillin 1 antibody [EPR27362-78] - BSA and Azide free (ab315385), expandable thumbnail](https://content.abcam.com/products/images/fibrillin-1-antibody-epr27362-78-bsa-and-azide-free-ab315385--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img424659.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrillin 1 antibody [EPR27362-78] - BSA and Azide free (ab315385)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrillin 1 antibody [EPR27362-78] - BSA and Azide free (ab315385)
This data was developed using Anti-Fibrillin 1 antibody [EPR27362-78] ab315384, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling Fibrillin 1 with Anti-Fibrillin 1 antibody [EPR27362-78] ab315384 at 1/100 (5.08 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on stromal connective tissue of human hepatocellular carcinoma. The section was incubated with Anti-Fibrillin 1 antibody [EPR27362-78] ab315384 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with citrate buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrillin 1 antibody [EPR27362-78] - BSA and Azide free (ab315385), expandable thumbnail](https://content.abcam.com/products/images/fibrillin-1-antibody-epr27362-78-bsa-and-azide-free-ab315385--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img424658.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrillin 1 antibody [EPR27362-78] - BSA and Azide free (ab315385)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrillin 1 antibody [EPR27362-78] - BSA and Azide free (ab315385)
This data was developed using Anti-Fibrillin 1 antibody [EPR27362-78] ab315384, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling Fibrillin 1 with Anti-Fibrillin 1 antibody [EPR27362-78] ab315384 at 1/100 (5.08 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on stromal connective tissue of human testis. The section was incubated with Anti-Fibrillin 1 antibody [EPR27362-78] ab315384 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with citrate buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrillin 1 antibody [EPR27362-78] - BSA and Azide free (ab315385), expandable thumbnail](https://content.abcam.com/products/images/fibrillin-1-antibody-epr27362-78-bsa-and-azide-free-ab315385--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img424657.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrillin 1 antibody [EPR27362-78] - BSA and Azide free (ab315385)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrillin 1 antibody [EPR27362-78] - BSA and Azide free (ab315385)
This data was developed using Anti-Fibrillin 1 antibody [EPR27362-78] ab315384, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human skin tissue labeling Fibrillin 1 with Anti-Fibrillin 1 antibody [EPR27362-78] ab315384 at 1/100 (5.08 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on stromal connective tissue of human skin (PMID: 16120151, PMID: 20195245). The section was incubated with Anti-Fibrillin 1 antibody [EPR27362-78] ab315384 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with citrate buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
![Immunocytochemistry/ Immunofluorescence - Anti-Fibrillin 1 antibody [EPR27362-78] - BSA and Azide free (ab315385), expandable thumbnail](https://content.abcam.com/products/images/fibrillin-1-antibody-epr27362-78-bsa-and-azide-free-ab315385--immunocytochemistry-immunofluorescence-img424707.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Fibrillin 1 antibody [EPR27362-78] - BSA and Azide free (ab315385)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrillin 1 antibody [EPR27362-78] - BSA and Azide free (ab315385), expandable thumbnail](https://content.abcam.com/products/images/fibrillin-1-antibody-epr27362-78-bsa-and-azide-free-ab315385--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img424660.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrillin 1 antibody [EPR27362-78] - BSA and Azide free (ab315385)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrillin 1 antibody [EPR27362-78] - BSA and Azide free (ab315385), expandable thumbnail](https://content.abcam.com/products/images/fibrillin-1-antibody-epr27362-78-bsa-and-azide-free-ab315385--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img424659.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrillin 1 antibody [EPR27362-78] - BSA and Azide free (ab315385)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrillin 1 antibody [EPR27362-78] - BSA and Azide free (ab315385), expandable thumbnail](https://content.abcam.com/products/images/fibrillin-1-antibody-epr27362-78-bsa-and-azide-free-ab315385--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img424658.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrillin 1 antibody [EPR27362-78] - BSA and Azide free (ab315385)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrillin 1 antibody [EPR27362-78] - BSA and Azide free (ab315385), expandable thumbnail](https://content.abcam.com/products/images/fibrillin-1-antibody-epr27362-78-bsa-and-azide-free-ab315385--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img424657.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrillin 1 antibody [EPR27362-78] - BSA and Azide free (ab315385)")
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com