AB108616

Anti-Fibrinogen alpha chain antibody [EPR2918]

RabMAb
Recombinant
What is this?

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(3 Publications)

Rabbit Recombinant Monoclonal Fibrinogen alpha chain antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications.

View Alternative Names

Fibrinogen alpha chain, FGA

2 Images

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Western blot - Anti-Fibrinogen alpha chain antibody [EPR2918] (AB108616)

All lanes:

Western blot - Anti-Fibrinogen alpha chain antibody [EPR2918] (ab108616) at 1/2000 dilution

Lane 1:

Human plasma lysates at 10 µg

Lane 2:

Human fetal liver lysates at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 95 kDa

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Western blot - Anti-Fibrinogen alpha chain antibody [EPR2918] (AB108616)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-Fibrinogen alpha chain antibody [EPR2918] (ab108616) at 0.9 µg/mL

All lanes:

Human plasma lysates at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 95 kDa

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Carrier free

Anti-Fibrinogen alpha chain antibody [EPR2918] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2918

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The immunogen is derived from isoform alpha-E, UniProt accession P02671-1, and the antibody is not expected to detect isoform alpha.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cleaved by the protease thrombin to yield monomers which, together with fibrinogen beta (FGB) and fibrinogen gamma (FGG), polymerize to form an insoluble fibrin matrix. Fibrin has a major function in hemostasis as one of the primary components of blood clots. In addition, functions during the early stages of wound repair to stabilize the lesion and guide cell migration during re-epithelialization. Was originally thought to be essential for platelet aggregation, based on in vitro studies using anticoagulated blood. However, subsequent studies have shown that it is not absolutely required for thrombus formation in vivo. Enhances expression of SELP in activated platelets via an ITGB3-dependent pathway. Maternal fibrinogen is essential for successful pregnancy. Fibrin deposition is also associated with infection, where it protects against IFNG-mediated hemorrhage. May also facilitate the immune response via both innate and T-cell mediated pathways.

See full target information FGA

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in genetics 11:551065 PubMed33424913

2020

Integration of Transcriptome Resequencing and Quantitative Proteomics Analyses of Collagenase VII-Induced Intracerebral Hemorrhage in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Fang Cao,Yu Guo,Qiang Zhang,Yinchun Fan,Qian Liu,Jiancheng Song,Hua Zhong,Shengtao Yao

Proteomics. Clinical applications 14:e1900099 PubMed32677377

2020

Isoform 1 of Fibrinogen Alpha Chain Precursor is a Potential Biomarker for Steroid-Induced Osteonecrosis of the Femoral Head.

Applications

Unspecified application

Species

Unspecified reactive species

Liying Liu,Jidong Song,Jun Li,Na Huang,Juan Yang,Shugang Hu,Rui Ma,Wei Wang

Blood advances 4:2939-2949 PubMed32603423

2020

Dominant role of αIIbβ3 in platelet interactions with cross-linked fibrin fragment D-dimer.

Applications

Unspecified application

Species

Unspecified reactive species

Lorena Buitrago,Hina Zafar,Yixiao Zhang,Jihong Li,Thomas Walz,Barry S Coller

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Fibrinogen alpha chain antibody [EPR2918]

Western blot - Anti-Fibrinogen alpha chain antibody [EPR2918] (AB108616)

Western blot - Anti-Fibrinogen alpha chain antibody [EPR2918] (AB108616)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (3)

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