AB19079

Anti-Fibrinogen alpha chain antibody [UC45]

Name: Anti-Fibrinogen alpha chain antibody [UC45] (ab19079) | Abcam
Brand: Abcam Limited
SKU: AB19079
Price: 565 USD
Availability: InStock
Rating: 4 (2 reviews)

(2 Reviews)

(10 Publications)

Mouse Monoclonal Fibrinogen alpha chain antibody. Suitable for ICC, Flow Cyt, ELISA, RIA and reacts with Mouse, Rat, Human samples. Cited in 10 publications. Immunogen corresponding to Cell preparation containing FGA protein.

View Alternative Names

Fibrinogen alpha chain, FGA

1 Images

Flow Cytometry - Anti-Fibrinogen alpha chain antibody [UC45] (AB19079)

Flow Cyt

Unknown

Flow Cytometry - Anti-Fibrinogen alpha chain antibody [UC45] (AB19079)

Overlay histogram showing THP1 cells stained with ab19079 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab19079, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgM (mu chain) (ab97007) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgM [ICIGM] (ab91545, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

UC45

Isotype

IgM

Carrier free

Reacts with

Human, Rat, Mouse

Applications

Flow Cyt, RIA, ICC, ELISA

applications

Immunogen

Cell preparation containing FGA protein. The exact immunogen used to generate this antibody is proprietary information.

P02671

Specificity

This antibody reacts very strongly with the alpha chain of human fibrinogen, for further information please see Hogg 1983 paper quoted.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "RIA" : {"fullname" : "Radioimmunoassay", "shortname":"RIA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igm-b11-7-isotype-control-ab91545'>ab91545</a> - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "RIA-species-checked": "guaranteed", "RIA-species-dilution-info": "", "RIA-species-notes": "" }, "Mouse": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "RIA-species-checked": "guaranteed", "RIA-species-dilution-info": "", "RIA-species-notes": "" }, "Rat": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "RIA-species-checked": "guaranteed", "RIA-species-dilution-info": "", "RIA-species-notes": "" } } }

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Product details

A monocyte and neuronal antigen present predominantly on a protein of 45kDa MW. UC45 is specific for monocytic leukemias. The relevant antigens are not expressed in all cases with monocytic differentiation.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Purified from tissue culture supernatant.

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cleaved by the protease thrombin to yield monomers which, together with fibrinogen beta (FGB) and fibrinogen gamma (FGG), polymerize to form an insoluble fibrin matrix. Fibrin has a major function in hemostasis as one of the primary components of blood clots. In addition, functions during the early stages of wound repair to stabilize the lesion and guide cell migration during re-epithelialization. Was originally thought to be essential for platelet aggregation, based on in vitro studies using anticoagulated blood. However, subsequent studies have shown that it is not absolutely required for thrombus formation in vivo. Enhances expression of SELP in activated platelets via an ITGB3-dependent pathway. Maternal fibrinogen is essential for successful pregnancy. Fibrin deposition is also associated with infection, where it protects against IFNG-mediated hemorrhage. May also facilitate the immune response via both innate and T-cell mediated pathways.

See full target information FGA

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Artificial cells, nanomedicine, and biotechnology 48:28-36 PubMed31852256

2019

Gellable silk fibroin-polyethylene sponge for hemostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Wei,Jian Liu,ZhiBin Peng,Min Liang,YanSong Wang,XiaoQin Wang

Cancer science 110:2658-2666 PubMed31199029

2019

Novel strategy of ovarian cancer implantation: Pre-invasive growth of fibrin-anchored cells with neovascularization.

Applications

Unspecified application

Species

Unspecified reactive species

Ayumi Matsuoka,Yasunari Mizumoto,Masanori Ono,Kyosuke Kagami,Takeshi Obata,Junpei Terakawa,Yoshiko Maida,Mitsuhiro Nakamura,Takiko Daikoku,Hiroshi Fujiwara

Experimental and therapeutic medicine 18:1417-1425 PubMed31316628

2019

Effect of haptoglobin on the treatment of chronic hepatitis B with interferon.

Applications

Unspecified application

Species

Unspecified reactive species

Lin-Ling Lv,Yi-Xuan Yang,Tong-Dong Shi

Eye (London, England) 30:1389-1394 PubMed27367746

2016

Fibrin glue inhibits migration of ocular surface epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

A M Yeung,L A Faraj,O D McIntosh,V K Dhillon,H S Dua

American journal of nuclear medicine and molecular imaging 4:525-36 PubMed25250202

2014

Peptide targeted high-resolution molecular imaging of prostate cancer with MRI.

Applications

Unspecified application

Species

Unspecified reactive species

Xueming Wu,Guanping Yu,Daniel Lindner,Susann M Brady-Kalnay,Qi Zhang,Zheng-Rong Lu

International journal of molecular epidemiology an 5:53-70 PubMed24959311

2014

Practical detection of a definitive biomarker panel for Alzheimer's disease; comparisons between matched plasma and cerebrospinal fluid.

Applications

ELISA

Species

Human

Joanna L Richens,Kelly-Ann Vere,Roger A Light,Daniele Soria,Jonathan Garibaldi,A David Smith,Donald Warden,Gordon Wilcock,Nin Bajaj,Kevin Morgan,Paul O'Shea

American journal of nuclear medicine and molecular 3:446-55 PubMed24116353

2013

Molecular MRI of atherosclerotic plaque progression in an ApoE(-/-) mouse model with a CLT1 peptide targeted macrocyclic Gd(III) chelate.

Applications

Unspecified application

Species

Unspecified reactive species

Xueming Wu,Niranjan Balu,Wen Li,Yong Chen,Xiaoyue Shi,China M Kummitha,Xin Yu,Chun Yuan,Zheng-Rong Lu

Blood 63:566-73 PubMed6365201

1984

Monoclonal antibodies differentiating between monocytic and nonmonocytic variants of AML.

Applications

Unspecified application

Species

Unspecified reactive species

D C Linch,C Allen,P C Beverley,A G Bynoe,C S Scott,N Hogg

The Journal of experimental medicine 157:473-85 PubMed6822786

1983

Human monocytes are associated with the formation of fibrin.

Applications

Unspecified application

Species

Unspecified reactive species

N Hogg

Cell 24:875-84 PubMed6788378

1981

Monoclonal antibody with specificity for monocytes and neurons.

Applications

Unspecified application

Species

Unspecified reactive species

N Hogg,M Slusarenko,J Cohen,J Reiser

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Fibrinogen alpha chain antibody [UC45]

Flow Cytometry - Anti-Fibrinogen alpha chain antibody [UC45] (AB19079)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (10)

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