Anti-Fibrinogen rabbit polyclonal antibody that is used in Fibrinogen IHC, ICC/IF, ELISA, and more. Suitable for Human, Mouse and other species.
- Cited in over 60 publications
- Trusted since 2006
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- International journal of molecular sciences 24:2023The Effect of Leukocyte- and Platelet-Rich Fibrin on Central and Peripheral Nervous System Neurons-Implications for Biomaterial Applicability.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIvo Lambrichts,Esther Wolfs,Annelies Bronckaers,Pascal Gervois,Tim VangansewinkelPubMed 37762617
- Nature methods 20:1573-15802023GelMap: intrinsic calibration and deformation mapping for expansion microscopy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHugo G J Damstra,Josiah B Passmore,Albert K Serweta,Ioannis Koutlas,Mithila Burute,Frank J Meye,Anna Akhmanova,Lukas C KapiteinPubMed 37723243
- Scientific reports 13:145682023Association of clot ultrastructure with clot perviousness in stroke patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYoung Dae Kim,Il Kwon,Youngseon Park,Hyungwoo Lee,Il Hyung Lee,In Hwan Lim,Soon-Ho Hong,Hye Sun Lee,Hyo Suk Nam,Ji Hoe HeoPubMed 37666907
- Molecular neurodegeneration 18:592023Amyloid-β (Aβ) immunotherapy induced microhemorrhages are associated with activated perivascular macrophages and peripheral monocyte recruitment in Alzheimer's disease mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXavier Taylor,Isaiah M Clark,Griffin J Fitzgerald,Herold Oluoch,Justin T Hole,Ronald B DeMattos,Yaming Wang,Feng PanPubMed 37649100
- Scientific reports 13:104012023Shotgun proteomics identification of proteins expressed in the Descemet's membrane of patients with Fuchs endothelial corneal dystrophy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTatsuya Nakagawa,Naoki Okumura,Masaya Ikegawa,Yumiko Toyama,Takashi Nirasawa,Frederic Mascarelli,Hanielle Vaitinadapoule,Ines Aouimeur,Zhiguo He,Philippe Gain,Gilles Thuret,Noriko KoizumiPubMed 37369713
- International journal of molecular sciences 24:2023Extract Inhibits Platelet Desialylation and Activation to Ameliorate Sepsis-Associated Thrombocytopenia in a Murine CLP-Induced Sepsis Model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSeong-Hun Jeong,Ji-Young Park,Young Bae Ryu,Woo Sik Kim,In-Chul Lee,Ju-Hong Kim,Dohoon Kim,Ji-Hye Ha,Ba-Wool Lee,Jiyoung Nam,Kyoung-Oh Cho,Hyung-Jun KwonPubMed 37240208
- Molecular neurodegeneration 18:172023Astrocytic APOE4 removal confers cerebrovascular protection despite increased cerebral amyloid angiopathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMonica Xiong,Chao Wang,Maud Gratuze,Fareeha Saadi,Xin Bao,Megan E Bosch,Choonghee Lee,Hong Jiang,Javier Remolina Serrano,Ernesto R Gonzales,Michal Kipnis,David M HoltzmanPubMed 36922879
- Journal of inflammation research 16:1027-10422023Pretreatment with Eupatilin Attenuates Inflammation and Coagulation in Sepsis by Suppressing JAK2/STAT3 Signaling Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYilun Lu,Ding Li,Yueyue Huang,Yuanyuan Sun,Hongmin Zhou,Fanrong Ye,Hongjing Yang,Tingting Xu,Shichao Quan,Jingye PanPubMed 36926276
- Scientific reports 13:31032023Plasminogen activator inhibitor 1 is not a major causative factor for exacerbation in a mouse model of SARS-CoV-2 infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTakashin Nakayama,Tatsuhiko Azegami,Maki Kiso,Masaki Imai,Ryuta Uraki,Kaori Hayashi,Akihito Hishikawa,Norifumi Yoshimoto,Ran Nakamichi,Erina Sugita-Nishimura,Eriko Yoshida-Hama,Yoshihiro Kawaoka,Hiroshi ItohPubMed 36813909
- Autophagy 19:1803-18202023Endothelial TFEB signaling-mediated autophagic disturbance initiates microglial activation and cognitive dysfunction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYaping Lu,Xiang Chen,Xiuxiu Liu,Yi Shi,Zhaocong Wei,Lili Feng,Quan Jiang,Weifeng Ye,Takuya Sasaki,Kohji Fukunaga,Yong Ji,Feng Han,Ying-Mei LuPubMed 36588318
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: Whole serum
- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Native Full Length Protein corresponding to Human FGA. Database link P02671
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Reactivity data
Select an application| IHC-P | sELISA | FuncS | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Expected | Expected | Expected |
| Mouse | Predicted | Predicted | Expected | Expected |
| Rat | Predicted | Predicted | Predicted | Predicted |
| Rabbit | Predicted | Predicted | Expected | Expected |
| Recombinant full length protein - Human | Not recommended | Tested | Not recommended | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Rabbit, Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
sELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes For sandwich ELISA, use this antibody as Detection at 1/200 dilution with Mouse monoclonal [1F7] to Fibrinopeptide A (ab14790) as Capture. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Rabbit, Mouse | Dilution info - | Notes - |
FuncS
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes This antibody has been tested in plasma clotting assays. The plasma clotting time was prolonged (but not completely neutralized) by the antibody. Testing has indicated that these neutralisation assays work only when this antibody has been protein A column purified first. |
Species Human | Dilution info - | Notes This antibody has been tested in plasma clotting assays. The plasma clotting time was prolonged (but not completely neutralized) by the antibody. Testing has indicated that these neutralisation assays work only when this antibody has been protein A column purified first. |
Species Rabbit | Dilution info - | Notes This antibody has been tested in plasma clotting assays. The plasma clotting time was prolonged (but not completely neutralized) by the antibody. Testing has indicated that these neutralisation assays work only when this antibody has been protein A column purified first. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rabbit | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Associated Products
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Target data
Function
Cleaved by the protease thrombin to yield monomers which, together with fibrinogen beta (FGB) and fibrinogen gamma (FGG), polymerize to form an insoluble fibrin matrix. Fibrin has a major function in hemostasis as one of the primary components of blood clots. In addition, functions during the early stages of wound repair to stabilize the lesion and guide cell migration during re-epithelialization. Was originally thought to be essential for platelet aggregation, based on in vitro studies using anticoagulated blood. However, subsequent studies have shown that it is not absolutely required for thrombus formation in vivo. Enhances expression of SELP in activated platelets via an ITGB3-dependent pathway. Maternal fibrinogen is essential for successful pregnancy. Fibrin deposition is also associated with infection, where it protects against IFNG-mediated hemorrhage. May also facilitate the immune response via both innate and T-cell mediated pathways.
Alternative names
Fibrinogen alpha chain, FGA
Recommended products
Anti-Fibrinogen rabbit polyclonal antibody that is used in Fibrinogen IHC, ICC/IF, ELISA, and more. Suitable for Human, Mouse and other species.
- Cited in over 60 publications
- Trusted since 2006
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: Whole serum
- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Native Full Length Protein corresponding to Human FGA. Database link P02671
- Purity
- Whole antiserum
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
Notes
Anti-Fibrinogen antibody (ab34269) is a rabbit polyclonal antibody and is validated for use in FuncS, ICC/IF, IHC-P, sELISA in human, mouse samples.
Anti-Fibrinogen antibody (ab34269) has been cited over 69 times in peer reviewed journals and is trusted by the scientific community.
Abcam's high quality validation processes ensure Anti-Fibrinogen antibody (ab34269) has high sensitivity and specificity.
Anti-Fibrinogen antibody (ab34269) has 12 independent reviews from customers.
Anti-Fibrinogen antibody (ab34269) specifically detects Fibrinogen (UniProt ID: P02671; Molecular weight: 2kDa) and is sold in 125 µL selling sizes.
Fibrinogen is a crucial protein involved in blood clotting and coagulation. Utilizing fibrinogen antibody can significantly enhance the detection and study of fibrinogen levels. Elevated fibrinogen levels are associated with increased risk of cardiovascular disease and inflammation, making it a vital biomarker for assessing health and disease.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
This image is courtesy of an anonymous customer review.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen antibody (ab34269)
ab34269 staining Fibrinogen in Human liver/placenta tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in formaldehyde and subjected to heat-mediated antigen retrieval in citrate buffer, pH 6.0 for 20 minutes at 100°C. The primary antibody was diluted 1/2000 and incubated with the sample for 20 minutes at 25°C. An HRP polymer-conjugated mouse anti-rabbit polyclonal IgG was used as the secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen antibody (ab34269)
ab34269 staining human liver. Staining is localized to the cytoplasm.
Left panel: with primary antibody at 1/50. Right panel: isotype control.
Sections were stained using an automated system (Dako PT Link), at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer, citrate pH 6.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
Sandwich ELISA - Anti-Fibrinogen antibody (ab34269)
This image is courtesy of an anonymous customer reviewImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen antibody (ab34269)
Immunohistochemical analysis of rat heart, staining Fibrinogen with ab34269.
Tissue was fixed with formaldehyde and blocked with 0.25% BSA for 15 minutes at room temperature; antigen retrieval was by heat mediation in citrate buffer. Samples were incubated with primary antibody (1.5 μg/ml in 1% BSA in TBS) for 20 minutes. An undiluted HRP-conjugated goat anti-rabbit polyclonal IgG was used as the secondary antibody.
This image is courtesy of an anonymous customer reviewImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen antibody (ab34269)
Immunohistochemistry analysis (Formalin/PFA-fixed paraffin-embedded sections) of paraformaldehyde fixed mouse liver tissue. Stained with ab34269 at 1/50 dilution.
Secondary antibody used was EnVision+ System- HRP Labelled Polymer anti rabbit. Blocking was done with 5% serum for 1 hour at 21°C. The sample was incubated with the primary antibody and 5% normal goat serum for 19 hours at 4°C. Antigen retrieval method was heat mediated Tris/EDTA pH 9.0
This image is courtesy of an anonymous customer reviewImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen antibody (ab34269)
Immunohistochemistry analysis (Formalin/PFA-fixed paraffin-embedded sections) of paraformaldehyde fixed human placenta tissue. Stained with ab34269 at 1/50 dilution. Secondary antibody used was EnVision+ System- HRP Labelled Polymer anti rabbit. Blocking was done with 5% serum for 1 hour at 21°C. The sample was incubated with the primary antibody and 5% normal goat serum for 19 hours at 4°C. Antigen retrieval method was heat mediated Tris/EDTA pH 9.0
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