Rabbit Multiclonal Fibrinogen alpha chain antibody. Suitable for WB and reacts with Human, Recombinant fragment - Human samples. Immunogen corresponding to Synthetic Peptide within Human FGA.
Preservative: 0.09% Sodium azide
Constituents: 99.91% PBS
This product was produced with the following immunogens:
WB | |
---|---|
Human | Tested |
Recombinant fragment - Human | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Species Recombinant fragment - Human | Dilution info 1 µg/mL | Notes - |
Cleaved by the protease thrombin to yield monomers which, together with fibrinogen beta (FGB) and fibrinogen gamma (FGG), polymerize to form an insoluble fibrin matrix. Fibrin has a major function in hemostasis as one of the primary components of blood clots. In addition, functions during the early stages of wound repair to stabilize the lesion and guide cell migration during re-epithelialization. Was originally thought to be essential for platelet aggregation, based on in vitro studies using anticoagulated blood. However, subsequent studies have shown that it is not absolutely required for thrombus formation in vivo. Enhances expression of SELP in activated platelets via an ITGB3-dependent pathway. Maternal fibrinogen is essential for successful pregnancy. Fibrin deposition is also associated with infection, where it protects against IFNG-mediated hemorrhage. May also facilitate the immune response via both innate and T-cell mediated pathways.
Fibrinogen alpha chain, FGA
Rabbit Multiclonal Fibrinogen alpha chain antibody. Suitable for WB and reacts with Human, Recombinant fragment - Human samples. Immunogen corresponding to Synthetic Peptide within Human FGA.
Preservative: 0.09% Sodium azide
Constituents: 99.91% PBS
This product was produced with the following immunogens:
What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:
View our range of recombinant multiclonal antibodies.
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We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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Western blot analysis of recombinant fibrinogen protein using ab308109 at a dilution of 1 µg/mL. Detection was performed using an HRP-conjugated Goat anti-Rabbit secondary antibody followed by chemiluminescence (ECL). Results show a band at ~340kDa
All lanes: Western blot - Anti-Fibrinogen Antibody [RP23040077] (ab308109) at 1 µg/mL
All lanes: Recombinant fibrinogen protein
All lanes: HRP-conjugated Goat anti-Rabbit secondary antibody
Developed using the ECL technique.
Observed band size: 340 kDa
Western blot was performed using ab308109 and bands around 70 and 51 kDa corresponding to Fibrinogen alpha and gamma chain were observed in human plasma. 10 µl of human plasma was electrophoresed using a 4-12% Bis-Tris gel, electrophoresis system and pre-stained protein standard. Resolved proteins were then transferred onto a nitrocellulose membrane. The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Recombinant Secondary Antibody conjugated to HRP (0.25 µg/mL, 1:4000 dilution). Chemiluminescent detection was performed (ECL).
All lanes: Western blot - Anti-Fibrinogen Antibody [RP23040077] (ab308109) at 1/1000 dilution
All lanes: Human plasma at 10 µL
All lanes: Goat anti-Rabbit IgG (H+L), Recombinant Secondary Antibody, HRP conjugated at 1/4000 dilution
Developed using the ECL technique.
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