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Rabbit Multiclonal Fibrinogen alpha chain antibody. Suitable for WB and reacts with Human, Recombinant fragment - Human samples. Immunogen corresponding to Synthetic Peptide within Human FGA.

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Images

Western blot - Anti-Fibrinogen Antibody [RP23040077] (AB308109), expandable thumbnail
  • Western blot - Anti-Fibrinogen Antibody [RP23040077] (AB308109), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Preservative: 0.09% Sodium azide
Constituents: 99.91% PBS

Form
Liquid
Clonality
Multiclonal

Immunogens

This product was produced with the following immunogens:

  • Synthetic Peptide within Human FGA. Database link P02671
  • Synthetic Peptide within Human FGA. Database link P02671

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested
Recombinant fragment - Human
Tested

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Species
Recombinant fragment - Human
Dilution info
1 µg/mL
Notes

-

Target data

Function

Cleaved by the protease thrombin to yield monomers which, together with fibrinogen beta (FGB) and fibrinogen gamma (FGG), polymerize to form an insoluble fibrin matrix. Fibrin has a major function in hemostasis as one of the primary components of blood clots. In addition, functions during the early stages of wound repair to stabilize the lesion and guide cell migration during re-epithelialization. Was originally thought to be essential for platelet aggregation, based on in vitro studies using anticoagulated blood. However, subsequent studies have shown that it is not absolutely required for thrombus formation in vivo. Enhances expression of SELP in activated platelets via an ITGB3-dependent pathway. Maternal fibrinogen is essential for successful pregnancy. Fibrin deposition is also associated with infection, where it protects against IFNG-mediated hemorrhage. May also facilitate the immune response via both innate and T-cell mediated pathways.

Alternative names

Recommended products

Rabbit Multiclonal Fibrinogen alpha chain antibody. Suitable for WB and reacts with Human, Recombinant fragment - Human samples. Immunogen corresponding to Synthetic Peptide within Human FGA.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Multiclonal
Immunogens

This product was produced with the following immunogens:

  • Synthetic Peptide within Human FGA. Database link P02671
  • Synthetic Peptide within Human FGA. Database link P02671
Clone number
RP23040077
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

  • - The sensitivity of polyclonal antibodies by recognising multiple epitopes
  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

View our range of recombinant multiclonal antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-Fibrinogen Antibody [RP23040077] (ab308109), expandable thumbnail

    Western blot - Anti-Fibrinogen Antibody [RP23040077] (ab308109)

    Western blot analysis of recombinant fibrinogen protein using ab308109 at a dilution of 1 µg/mL. Detection was performed using an HRP-conjugated Goat anti-Rabbit secondary antibody followed by chemiluminescence (ECL). Results show a band at ~340kDa

    All lanes: Western blot - Anti-Fibrinogen Antibody [RP23040077] (ab308109) at 1 µg/mL

    All lanes: Recombinant fibrinogen protein

    Secondary

    All lanes: HRP-conjugated Goat anti-Rabbit secondary antibody

    Developed using the ECL technique.

    Observed band size: 340 kDa

  • Western blot - Anti-Fibrinogen Antibody [RP23040077] (ab308109), expandable thumbnail

    Western blot - Anti-Fibrinogen Antibody [RP23040077] (ab308109)

    Western blot was performed using ab308109 and bands around 70 and 51 kDa corresponding to Fibrinogen alpha and gamma chain were observed in human plasma. 10 µl of human plasma was electrophoresed using a 4-12% Bis-Tris gel, electrophoresis system and pre-stained protein standard. Resolved proteins were then transferred onto a nitrocellulose membrane. The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Recombinant Secondary Antibody conjugated to HRP (0.25 µg/mL, 1:4000 dilution). Chemiluminescent detection was performed (ECL).

    All lanes: Western blot - Anti-Fibrinogen Antibody [RP23040077] (ab308109) at 1/1000 dilution

    All lanes: Human plasma at 10 µL

    Secondary

    All lanes: Goat anti-Rabbit IgG (H+L), Recombinant Secondary Antibody, HRP conjugated at 1/4000 dilution

    Developed using the ECL technique.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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