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AB288415

Anti-Fibrinogen beta chain antibody [8E5]

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Rabbit Recombinant Monoclonal Fibrinogen beta chain antibody. Suitable for Flow Cyt and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human Fibrinogen beta chain.

View Alternative Names

Fibrinogen beta chain, FGB

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Flow Cytometry - Anti-Fibrinogen beta chain antibody [8E5] (AB288415)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-Fibrinogen beta chain antibody [8E5] (AB288415)

Flow-cytometry using ab288415

HepG2 cells were fixed using 2% PFA, permeabilised using 0.5% Triton and stained with anti-Fluorescein IgG antibody (isotype control, black line) or the rabbit IgG-chimeric version of 8E5 (ab288415, blue line) at a dilution of 1 : 100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1 : 1000 and cells analyzed using a FACSCanto flow-cytometer.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

8E5

Isotype

IgG

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Synthetic Peptide within Human Fibrinogen beta chain. The exact immunogen used to generate this antibody is proprietary information.

P02675

Specificity

8E5 binds to the amino-terminal region of the β-chain of human fibrin at an epitope consiting of GHRPLDKC. Binding is possible in both the monomeric form and the polymeric form of the protein. The epitope recognised by this clone is only accessible upon conversion of fibrinogen to fibrin, which entails the proteolytic removal of fibrinopeptides A & B from fibrinogen by thrombin. The antibody is there is therefore fibrin specific.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/100", "FlowCyt-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.02% Proclin 300 Constituents: 99% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cleaved by the protease thrombin to yield monomers which, together with fibrinogen alpha (FGA) and fibrinogen gamma (FGG), polymerize to form an insoluble fibrin matrix. Fibrin has a major function in hemostasis as one of the primary components of blood clots. In addition, functions during the early stages of wound repair to stabilize the lesion and guide cell migration during re-epithelialization. Was originally thought to be essential for platelet aggregation, based on in vitro studies using anticoagulated blood. However subsequent studies have shown that it is not absolutely required for thrombus formation in vivo. Enhances expression of SELP in activated platelets. Maternal fibrinogen is essential for successful pregnancy. Fibrin deposition is also associated with infection, where it protects against IFNG-mediated hemorrhage. May also facilitate the antibacterial immune response via both innate and T-cell mediated pathways.
See full target information Fibrinogen beta chain
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Product promise

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