Rabbit Polyclonal Fibrinogen beta chain antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human Fibrinogen beta chain aa 250 to C-terminus.
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Publications
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- Journal of thrombosis and haemostasis : JTH 18:802-8142020Fibrinogen αC-regions are not directly involved in fibrin polymerization as evidenced by a "Double-Detroit" recombinant fibrinogen mutant and knobs-mimic peptides.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCédric Duval et. al.PubMed 31889430
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human Fibrinogen beta chain aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P02675
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Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Expected |
| Rat | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 - 1/10000 | Notes - |
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Associated Products
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Target data
Function
Cleaved by the protease thrombin to yield monomers which, together with fibrinogen alpha (FGA) and fibrinogen gamma (FGG), polymerize to form an insoluble fibrin matrix. Fibrin has a major function in hemostasis as one of the primary components of blood clots. In addition, functions during the early stages of wound repair to stabilize the lesion and guide cell migration during re-epithelialization. Was originally thought to be essential for platelet aggregation, based on in vitro studies using anticoagulated blood. However subsequent studies have shown that it is not absolutely required for thrombus formation in vivo. Enhances expression of SELP in activated platelets. Maternal fibrinogen is essential for successful pregnancy. Fibrin deposition is also associated with infection, where it protects against IFNG-mediated hemorrhage. May also facilitate the antibacterial immune response via both innate and T-cell mediated pathways.
Alternative names
Fibrinogen beta chain, FGB
Recommended products
Rabbit Polyclonal Fibrinogen beta chain antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human Fibrinogen beta chain aa 250 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human Fibrinogen beta chain aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P02675
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Western blot - Anti-Fibrinogen beta chain antibody (ab137830)
7.5% SDS PAGE
All lanes: Western blot - Anti-Fibrinogen beta chain antibody (ab137830) at 1/5000 dilution
All lanes: Molt 4 whole cell lysate at 30 µg
Predicted band size: 56 kDa
Western blot - Anti-Fibrinogen beta chain antibody (ab137830)
10% SDS PAGE
All lanes: Western blot - Anti-Fibrinogen beta chain antibody (ab137830) at 1/5000 dilution
All lanes: Mouse liver whole cell lysate at 50 µg
Predicted band size: 56 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Fibrinogen beta chain antibody (ab137830)
Immunofluorescence analysis of methanol-fixed HeLa cells, labelling Fibrinogen beta chain using ab137830 at 1/200 dilution. Bottom image is costained with Hoechst 33342.
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-Fibrinogen beta chain antibody (ab137830)
Fibrinogen beta chain western blot using anti-Fibrinogen beta chain antibody ab137830. Publication image and figure legend from Duval, C., Profumo, A., et al., 2020, J Thromb Haemost, PubMed 31889430.
ab137830 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab137830 please see the product overview.
Polymerization kinetics of fibrinogen preparations. (A) SDS‐PAGE on a 10% PAA gel under reducing conditions (two portions of the same gel) of control samples (hp‐FG, DD‐FG, and WT‐FG), samples from the turbidity experiment after thrombin (Thr) addition (DD‐FG + Thr and WT‐FG + Thr), and a molecular weight marker (MK). The normal FG chains positions are indicated on the left side; the standards molecular weights listed on the right side are only indicative. (B) turbidity time course of WT‐FG (blue trace), hp‐FG (magenta trace), and DD‐FG (red trace), all at 0.3 mg/mL, after activation with thrombin at 0.08 NHIu/mL (0.28 NIHu/mg FG)
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