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AB137830

Anti-Fibrinogen beta chain antibody

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(3 Publications)

Rabbit Polyclonal Fibrinogen beta chain antibody. Suitable for WB, ICC/IF and reacts with Rat, Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Fibrinogen beta chain aa 250 to C-terminus.

View Alternative Names

Fibrinogen beta chain, FGB

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-Fibrinogen beta chain antibody (AB137830)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Fibrinogen beta chain antibody (AB137830)

Immunofluorescence analysis of methanol-fixed HeLa cells, labelling Fibrinogen beta chain using ab137830 at 1/200 dilution. Bottom image is costained with Hoechst 33342.

Western blot - Anti-Fibrinogen beta chain antibody (AB137830)
  • WB

Supplier Data

Western blot - Anti-Fibrinogen beta chain antibody (AB137830)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-Fibrinogen beta chain antibody (ab137830) at 1/50000 dilution

All lanes:

Human plasma at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 56 kDa

false

Western blot - Anti-Fibrinogen beta chain antibody (AB137830)
  • WB

Supplier Data

Western blot - Anti-Fibrinogen beta chain antibody (AB137830)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-Fibrinogen beta chain antibody (ab137830) at 1/50000 dilution

All lanes:

Rat plasma at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 56 kDa

false

Western blot - Anti-Fibrinogen beta chain antibody (AB137830)
  • WB

Supplier Data

Western blot - Anti-Fibrinogen beta chain antibody (AB137830)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-Fibrinogen beta chain antibody (ab137830) at 1/50000 dilution

All lanes:

Mouse plasma at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 56 kDa

false

Western blot - Anti-Fibrinogen beta chain antibody (AB137830)
  • WB

CiteAb

Western blot - Anti-Fibrinogen beta chain antibody (AB137830)

Fibrinogen beta chain western blot using anti-Fibrinogen beta chain antibody ab137830. Publication image and figure legend from Duval, C., Profumo, A., et al., 2020, J Thromb Haemost, PubMed 31889430.

ab137830 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab137830 please see the product overview.

Polymerization kinetics of fibrinogen preparations. (A) SDS‐PAGE on a 10% PAA gel under reducing conditions (two portions of the same gel) of control samples (hp‐FG, DD‐FG, and WT‐FG), samples from the turbidity experiment after thrombin (Thr) addition (DD‐FG + Thr and WT‐FG + Thr), and a molecular weight marker (MK). The normal FG chains positions are indicated on the left side; the standards molecular weights listed on the right side are only indicative. (B) turbidity time course of WT‐FG (blue trace), hp‐FG (magenta trace), and DD‐FG (red trace), all at 0.3 mg/mL, after activation with thrombin at 0.08 NHIu/mL (0.28 NIHu/mg FG)

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human Fibrinogen beta chain aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P02675

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/50000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/50000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/50000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Cleaved by the protease thrombin to yield monomers which, together with fibrinogen alpha (FGA) and fibrinogen gamma (FGG), polymerize to form an insoluble fibrin matrix. Fibrin has a major function in hemostasis as one of the primary components of blood clots. In addition, functions during the early stages of wound repair to stabilize the lesion and guide cell migration during re-epithelialization. Was originally thought to be essential for platelet aggregation, based on in vitro studies using anticoagulated blood. However subsequent studies have shown that it is not absolutely required for thrombus formation in vivo. Enhances expression of SELP in activated platelets. Maternal fibrinogen is essential for successful pregnancy. Fibrin deposition is also associated with infection, where it protects against IFNG-mediated hemorrhage. May also facilitate the antibacterial immune response via both innate and T-cell mediated pathways.
See full target information Fibrinogen beta chain

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 43:114848 PubMed39392753

2024

Post-stroke hippocampal neurogenesis is impaired by microvascular dysfunction and PI3K signaling in cerebral amyloid angiopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Olivia M Osborne,Manav Daftari,Oandy Naranjo,Adarsh N Johar,Samantha Brooks,Brett M Colbert,Silvia Torices,Elizabeth Lewis,Jet Sendaydiego,Gillian Drexler,Malek Bashti,Alexander V Margetts,Luis M Tuesta,Christian Mason,Daniel Bilbao,Regina Vontell,Anthony J Griswold,Derek M Dykxhoorn,Michal Toborek

Nature cardiovascular research 2:438-448 PubMed39196046

2023

Genetic or therapeutic neutralization of ALK1 reduces LDL transcytosis and atherosclerosis in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Sungwoon Lee,Hubertus Schleer,Hyojin Park,Erika Jang,Michael Boyer,Bo Tao,Ana Gamez-Mendez,Abhishek Singh,Ewa Folta-Stogniew,Xinbo Zhang,Lingfeng Qin,Xue Xiao,Lin Xu,Junhui Zhang,Xiaoyue Hu,Evanthia Pashos,George Tellides,Philip W Shaul,Warren L Lee,Carlos Fernandez-Hernando,Anne Eichmann,William C Sessa

Journal of thrombosis and haemostasis : JTH 18:802-814 PubMed31889430

2020

Fibrinogen αC-regions are not directly involved in fibrin polymerization as evidenced by a "Double-Detroit" recombinant fibrinogen mutant and knobs-mimic peptides.

Applications

Unspecified application

Species

Unspecified reactive species

Cédric Duval,Aldo Profumo,Anna Aprile,Annalisa Salis,Enrico Millo,Gianluca Damonte,Julia S Gauer,Robert A S Ariëns,Mattia Rocco
View all publications

Product promise

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