Rabbit Recombinant Monoclonal Fibrinogen beta chain antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 8 publications.
Images
![Western blot - Anti-Fibrinogen beta chain antibody [EPR18145-84] (AB189490), expandable thumbnail](https://content.abcam.com/products/images/fibrinogen-beta-chain-antibody-epr18145-84-ab189490--western-blot-img16012.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Fibrinogen beta chain antibody [EPR18145-84] (AB189490)")
![Western blot - Anti-Fibrinogen beta chain antibody [EPR18145-84] (AB189490), expandable thumbnail](https://content.abcam.com/products/images/fibrinogen-beta-chain-antibody-epr18145-84-ab189490--western-blot-img29263.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Fibrinogen beta chain antibody [EPR18145-84] (AB189490)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen beta chain antibody [EPR18145-84] (AB189490), expandable thumbnail](https://content.abcam.com/products/images/fibrinogen-beta-chain-antibody-epr18145-84-ab189490--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img25656.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen beta chain antibody [EPR18145-84] (AB189490)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen beta chain antibody [EPR18145-84] (AB189490), expandable thumbnail](https://content.abcam.com/products/images/fibrinogen-beta-chain-antibody-epr18145-84-ab189490--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img65782.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen beta chain antibody [EPR18145-84] (AB189490)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen beta chain antibody [EPR18145-84] (AB189490), expandable thumbnail](https://content.abcam.com/products/images/fibrinogen-beta-chain-antibody-epr18145-84-ab189490--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img132515.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen beta chain antibody [EPR18145-84] (AB189490)")
Publications
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- Cell death & disease 14:7172023GRSF1 antagonizes age-associated hypercoagulability via modulation of fibrinogen mRNA stability.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDoudou Liu,Chenzhong Xu,Ze Gong,Yijie Zhao,Zhiqiang Fang,Xiaoli Rao,Qingyu Chen,Guodong Li,Wei Kong,Jun ChenPubMed 37923734
- Medical oncology (Northwood, London, England) 40:102022Fibrinogen promotes gallbladder cancer cell metastasis and extravasation by inducing ICAM1 expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChengkai Jiang,Yang Li,Yongsheng Li,Liguo Liu,Xu-An Wang,Wenguang Wu,Runfa Bao,Hao Weng,Maolan Li,Yajun Geng,Yijun Shu,Yingbin LiuPubMed 36352295
- Cell death & disease 13:2072022The mmu_circRNA_37492/hsa_circ_0012138 function as potential ceRNA to attenuate obstructive renal fibrosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXu Cheng,Kai Ai,Lei Yi,Wentao Liu,Yijian Li,Yinhuai Wang,Dongshan ZhangPubMed 35246505
- Cellular and molecular gastroenterology and hepatology 11:683-6962020Fibrinogen/AKT/Microfilament Axis Promotes Colitis by Enhancing Vascular Permeability.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChong Zhang,Honglv Chen,Qiaoling He,Yiqin Luo,Andong He,Ailin Tao,Jie YanPubMed 33075564
- Haematologica 106:2469-24772020Interleukin-1 receptor inhibition reduces stroke size in a murine model of sickle cell disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJessica Venugopal,Jintao Wang,Jalal Mawri,Chiao Guo,Daniel EitzmanPubMed 32817286
- Scientific reports 10:67652020The Prion-like protein Shadoo is involved in mouse embryonic and mammary development and differentiation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBruno Passet et. al.PubMed 32317725
- Biochemistry and biophysics reports 22:1007362020Short-term high-fat diet intake leads to exacerbation of concanavalin A-induced liver injury through the induction of procoagulation state.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEri Nanizawa et. al.PubMed 32083190
- Circulation research 126:162-1812019Activated Endothelial TGFβ1 Signaling Promotes Venous Thrombus Nonresolution in Mice Via Endothelin-1: Potential Role for Chronic Thromboembolic Pulmonary Hypertension.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMagdalena L Bochenek,Christiane Leidinger,Nico S Rosinus,Rajinikanth Gogiraju,Stefan Guth,Lukas Hobohm,Kerstin Jurk,Eckhard Mayer,Thomas Münzel,Mareike Lankeit,Markus Bosmann,Stavros Konstantinides,Katrin SchäferPubMed 31747868
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Expected |
| Mouse | Tested | Tested |
| Rat | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/1000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/4000 | Notes This antibody is not recommended for human species in IHC application due to nonspecific staining on human stromal cells. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/4000 | Notes This antibody is not recommended for human species in IHC application due to nonspecific staining on human stromal cells. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
Target data
Function
Cleaved by the protease thrombin to yield monomers which, together with fibrinogen alpha (FGA) and fibrinogen gamma (FGG), polymerize to form an insoluble fibrin matrix. Fibrin has a major function in hemostasis as one of the primary components of blood clots. In addition, functions during the early stages of wound repair to stabilize the lesion and guide cell migration during re-epithelialization. Was originally thought to be essential for platelet aggregation, based on in vitro studies using anticoagulated blood. However subsequent studies have shown that it is not absolutely required for thrombus formation in vivo. Enhances expression of SELP in activated platelets. Maternal fibrinogen is essential for successful pregnancy. Fibrin deposition is also associated with infection, where it protects against IFNG-mediated hemorrhage. May also facilitate the antibacterial immune response via both innate and T-cell mediated pathways.
Alternative names
Fibrinogen beta chain, FGB
Recommended products
Rabbit Recombinant Monoclonal Fibrinogen beta chain antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 8 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR18145-84
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Western blot - Anti-Fibrinogen beta chain antibody [EPR18145-84] (ab189490), expandable thumbnail](https://content.abcam.com/products/images/fibrinogen-beta-chain-antibody-epr18145-84-ab189490--western-blot-img16012.jpg "Western blot - Anti-Fibrinogen beta chain antibody [EPR18145-84] (ab189490)")
Western blot - Anti-Fibrinogen beta chain antibody [EPR18145-84] (ab189490)
Blocking and dilution buffer: 5% NFDM/TBST
Exposure times:
Lanes 1-4: 3 seconds
Lane 5: 2 seconds
Lane 6: 1 minute
All lanes: Western blot - Anti-Fibrinogen beta chain antibody [EPR18145-84] (ab189490) at 1/1000 dilution
Lane 1: Human liver lysate at 20 µg
Lane 2: Human plasma at 20 µg
Lane 3: Rat plasma at 20 µg
Lane 4: Rat platelet lysate at 20 µg
Lane 5: Mouse plasma at 20 µg
Lane 6: Rat liver lysate at 20 µg
SecondaryLanes 1 - 2: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/4000 dilution
Lanes 3 - 6: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 56 kDa
Observed band size: 55 kDa
![Western blot - Anti-Fibrinogen beta chain antibody [EPR18145-84] (ab189490), expandable thumbnail](https://content.abcam.com/products/images/fibrinogen-beta-chain-antibody-epr18145-84-ab189490--western-blot-img29263.jpg "Western blot - Anti-Fibrinogen beta chain antibody [EPR18145-84] (ab189490)")
Western blot - Anti-Fibrinogen beta chain antibody [EPR18145-84] (ab189490)
Blocking and dilution buffer: 5% NFDM/TBST
Exposure times:
Lane 1: 3 seconds
Lane 2: 1 second
Lane 3: 3 minutes
All lanes: Western blot - Anti-Fibrinogen beta chain antibody [EPR18145-84] (ab189490) at 1/1000 dilution
Lane 1: HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 10 µg
Lane 2: Rat liver lysate at 10 µg
Lane 3: Hepa1-6 (mouse hepatoma epithelial cell line) whole cell lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 56 kDa
Observed band size: 55 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen beta chain antibody [EPR18145-84] (ab189490), expandable thumbnail](https://content.abcam.com/products/images/fibrinogen-beta-chain-antibody-epr18145-84-ab189490--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img25656.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen beta chain antibody [EPR18145-84] (ab189490)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen beta chain antibody [EPR18145-84] (ab189490)
Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling Fibrinogen beta with ab189490 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on hepatocytes and blood vessels of mouse liver (PMID: 10467729; PMID: 3778466) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen beta chain antibody [EPR18145-84] (ab189490), expandable thumbnail](https://content.abcam.com/products/images/fibrinogen-beta-chain-antibody-epr18145-84-ab189490--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img65782.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen beta chain antibody [EPR18145-84] (ab189490)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen beta chain antibody [EPR18145-84] (ab189490)
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Fibrinogen beta with ab189490 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on hepatocytes and blood vessels of mouse liver (PMID: 3778466) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen beta chain antibody [EPR18145-84] (ab189490), expandable thumbnail](https://content.abcam.com/products/images/fibrinogen-beta-chain-antibody-epr18145-84-ab189490--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img132515.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen beta chain antibody [EPR18145-84] (ab189490)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen beta chain antibody [EPR18145-84] (ab189490)
Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling Fibrinogen beta with ab189490 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on hepatocytes and blood vessels of mouse liver (PMID: 10467729; PMID: 3778466) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen beta chain antibody [EPR18145-84] (ab189490), expandable thumbnail](https://content.abcam.com/products/images/fibrinogen-beta-chain-antibody-epr18145-84-ab189490--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img112464.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen beta chain antibody [EPR18145-84] (ab189490)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen beta chain antibody [EPR18145-84] (ab189490)
Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling Fibrinogen beta with ab189490 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on hepatocytes and blood vessels of mouse liver (PMID: 3778466) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen beta chain antibody [EPR18145-84] (ab189490), expandable thumbnail](https://content.abcam.com/products/images/fibrinogen-beta-chain-antibody-epr18145-84-ab189490--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img112464.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen beta chain antibody [EPR18145-84] (ab189490)")
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