Rabbit Recombinant Monoclonal Fibrinogen beta chain antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 3 publications.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen beta chain antibody [EPR18145-84] - BSA and Azide free (AB227063), expandable thumbnail](https://content.abcam.com/products/images/fibrinogen-beta-chain-antibody-epr18145-84-bsa-and-azide-free-ab227063--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img25656.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen beta chain antibody [EPR18145-84] - BSA and Azide free (AB227063)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen beta chain antibody [EPR18145-84] - BSA and Azide free (AB227063), expandable thumbnail](https://content.abcam.com/products/images/fibrinogen-beta-chain-antibody-epr18145-84-bsa-and-azide-free-ab227063--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img65782.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen beta chain antibody [EPR18145-84] - BSA and Azide free (AB227063)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen beta chain antibody [EPR18145-84] - BSA and Azide free (AB227063), expandable thumbnail](https://content.abcam.com/products/images/fibrinogen-beta-chain-antibody-epr18145-84-bsa-and-azide-free-ab227063--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img132515.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen beta chain antibody [EPR18145-84] - BSA and Azide free (AB227063)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen beta chain antibody [EPR18145-84] - BSA and Azide free (AB227063), expandable thumbnail](https://content.abcam.com/products/images/fibrinogen-beta-chain-antibody-epr18145-84-bsa-and-azide-free-ab227063--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img112464.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen beta chain antibody [EPR18145-84] - BSA and Azide free (AB227063)")
Publications
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- Frontiers in aging neuroscience 15:10890052023Preserved blood-brain barrier and neurovascular coupling in female 5xFAD model of Alzheimer's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOleg Zhukov,Chen He,Rana Soylu-Kucharz,Changsi Cai,Andreas D Lauritzen,Blanca Irene Aldana,Maria Björkqvist,Martin Lauritzen,Krzysztof KucharzPubMed 37261266
- Scientific reports 12:50122022Lung lymphatic thrombosis and dysfunction caused by cigarette smoke exposure precedes emphysema in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBarbara D Summers,Kihwan Kim,Cristina C Clement,Zohaib Khan,Sangeetha Thangaswamy,Jacob McCright,Katharina Maisel,Sofia Zamora,Stephanie Quintero,Alexandra C Racanelli,David Redmond,Jeanine D'Armiento,Jisheng Yang,Amy Kuang,Laurel Monticelli,Mark L Kahn,Augustine M K Choi,Laura Santambrogio,Hasina Outtz ReedPubMed 35322079
- eLife 8:2019Apolipoprotein M-bound sphingosine-1-phosphate regulates blood-brain barrier paracellular permeability and transcytosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMette Mathiesen Janiurek et. al.PubMed 31763978
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Expected | Predicted |
| Mouse | Expected | Tested |
| Rat | Expected | Predicted |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes - |
Species Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
Target data
Function
Cleaved by the protease thrombin to yield monomers which, together with fibrinogen alpha (FGA) and fibrinogen gamma (FGG), polymerize to form an insoluble fibrin matrix. Fibrin has a major function in hemostasis as one of the primary components of blood clots. In addition, functions during the early stages of wound repair to stabilize the lesion and guide cell migration during re-epithelialization. Was originally thought to be essential for platelet aggregation, based on in vitro studies using anticoagulated blood. However subsequent studies have shown that it is not absolutely required for thrombus formation in vivo. Enhances expression of SELP in activated platelets. Maternal fibrinogen is essential for successful pregnancy. Fibrin deposition is also associated with infection, where it protects against IFNG-mediated hemorrhage. May also facilitate the antibacterial immune response via both innate and T-cell mediated pathways.
Alternative names
Fibrinogen beta chain, FGB
Recommended products
Rabbit Recombinant Monoclonal Fibrinogen beta chain antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 3 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR18145-84
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab227063 is the carrier-free version of Anti-Fibrinogen beta chain antibody [EPR18145-84] ab189490.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen beta chain antibody [EPR18145-84] - BSA and Azide free (ab227063), expandable thumbnail](https://content.abcam.com/products/images/fibrinogen-beta-chain-antibody-epr18145-84-bsa-and-azide-free-ab227063--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img25656.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen beta chain antibody [EPR18145-84] - BSA and Azide free (ab227063)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen beta chain antibody [EPR18145-84] - BSA and Azide free (ab227063)
Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling Fibrinogen beta with Anti-Fibrinogen beta chain antibody [EPR18145-84] ab189490 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on hepatocytes and blood vessels of mouse liver (PMID: 10467729; PMID: 3778466) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Fibrinogen beta chain antibody [EPR18145-84] ab189490).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen beta chain antibody [EPR18145-84] - BSA and Azide free (ab227063), expandable thumbnail](https://content.abcam.com/products/images/fibrinogen-beta-chain-antibody-epr18145-84-bsa-and-azide-free-ab227063--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img65782.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen beta chain antibody [EPR18145-84] - BSA and Azide free (ab227063)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen beta chain antibody [EPR18145-84] - BSA and Azide free (ab227063)
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Fibrinogen beta with Anti-Fibrinogen beta chain antibody [EPR18145-84] ab189490 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on hepatocytes and blood vessels of mouse liver (PMID: 3778466) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Fibrinogen beta chain antibody [EPR18145-84] ab189490).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen beta chain antibody [EPR18145-84] - BSA and Azide free (ab227063), expandable thumbnail](https://content.abcam.com/products/images/fibrinogen-beta-chain-antibody-epr18145-84-bsa-and-azide-free-ab227063--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img132515.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen beta chain antibody [EPR18145-84] - BSA and Azide free (ab227063)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen beta chain antibody [EPR18145-84] - BSA and Azide free (ab227063)
Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling Fibrinogen beta with Anti-Fibrinogen beta chain antibody [EPR18145-84] ab189490 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on hepatocytes and blood vessels of mouse liver (PMID: 10467729; PMID: 3778466) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Fibrinogen beta chain antibody [EPR18145-84] ab189490).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen beta chain antibody [EPR18145-84] - BSA and Azide free (ab227063), expandable thumbnail](https://content.abcam.com/products/images/fibrinogen-beta-chain-antibody-epr18145-84-bsa-and-azide-free-ab227063--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img112464.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen beta chain antibody [EPR18145-84] - BSA and Azide free (ab227063)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen beta chain antibody [EPR18145-84] - BSA and Azide free (ab227063)
Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling Fibrinogen beta with Anti-Fibrinogen beta chain antibody [EPR18145-84] ab189490 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on hepatocytes and blood vessels of mouse liver (PMID: 3778466) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Fibrinogen beta chain antibody [EPR18145-84] ab189490).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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