AB193932

Anti-Fibrinogen beta chain antibody [EPR19765]

RabMAb
Recombinant
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(6 Publications)

Rabbit Recombinant Monoclonal Fibrinogen beta chain antibody. Suitable for IP, WB, ICC/IF and reacts with Human samples. Cited in 6 publications.

View Alternative Names

Fibrinogen beta chain, FGB

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-Fibrinogen beta chain antibody [EPR19765] (AB193932)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Fibrinogen beta chain antibody [EPR19765] (AB193932)

Immunofluorescent analysis of 4% PFA-fixed 0.1% Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells labeling Fibrinogen beta chain with ab193932 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining of Fibrinogen beta on HepG2 cells.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

Supplier Data

Immunoprecipitation - Anti-Fibrinogen beta chain antibody [EPR19765] (AB193932)

Fibrinogen beta chain was immunoprecipitated from 0.35 mg of HepG2 (human liver hepatocellular carcinoma cell line) lysate with ab193932 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab193932 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Lane 1 : HepG2 whole cell lysate 10 μg (Input).

Lane 2 : ab193932 IP in HepG2 whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab193932 in HepG2 whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 30 seconds.

All lanes:

Immunoprecipitation - Anti-Fibrinogen beta chain antibody [EPR19765] (ab193932)

Predicted band size: 56 kDa

Observed band size: 55 kDa

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Supplier Data

Western blot - Anti-Fibrinogen beta chain antibody [EPR19765] (AB193932)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Fibrinogen beta chain antibody [EPR19765] (ab193932) at 1/2000 dilution

Lane 1:

His-tagged human fibrinogen alpha chain recombinant protein (aa401-866) at 0.02 µg

Lane 2:

GST-His-tagged human fibrinogen beta chain recombinant protein (aa1-491) at 0.02 µg

Lane 3:

GST-His-tagged human fibrinogen gamma chain recombinant protein (aa1-453) at 0.02 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 56 kDa

Observed band size: 80 kDa

true

Exposure time: 1s

Supplier Data

Western blot - Anti-Fibrinogen beta chain antibody [EPR19765] (AB193932)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1 : 5 seconds; Lane 2 : 1 second; Lane 3 : 3 minutes.

All lanes:

Western blot - Anti-Fibrinogen beta chain antibody [EPR19765] (ab193932) at 1/1000 dilution

Lane 1:

Human fetal liver tissue lysate at 20 µg

Lane 2:

Human plasma at 20 µg

Lane 3:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg

Secondary

Lanes 1 - 2:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Lane 3:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 56 kDa

Observed band size: 55 kDa

true

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19765

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cleaved by the protease thrombin to yield monomers which, together with fibrinogen alpha (FGA) and fibrinogen gamma (FGG), polymerize to form an insoluble fibrin matrix. Fibrin has a major function in hemostasis as one of the primary components of blood clots. In addition, functions during the early stages of wound repair to stabilize the lesion and guide cell migration during re-epithelialization. Was originally thought to be essential for platelet aggregation, based on in vitro studies using anticoagulated blood. However subsequent studies have shown that it is not absolutely required for thrombus formation in vivo. Enhances expression of SELP in activated platelets. Maternal fibrinogen is essential for successful pregnancy. Fibrin deposition is also associated with infection, where it protects against IFNG-mediated hemorrhage. May also facilitate the antibacterial immune response via both innate and T-cell mediated pathways.

See full target information Fibrinogen beta chain

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

BMC infectious diseases 25:1252 PubMed41062954

2025

Bioinformatics analysis of the proteome in the pathway of complement and coagulation cascades in COVID-19: discovering potential biomarkers of FGB and SERPINA5.

Applications

Unspecified application

Species

Unspecified reactive species

Siyuan Liu,Huichun Shi,Yuexiang Yang,Cuisong Zhu,Jing Zhou,Wenzhong Jin,Shu Song,Lijun Zhang

Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics 22:e00538 PubMed39904669

2025

Fibrinogen degradation products exacerbate alpha-synuclein aggregation by inhibiting autophagy via downregulation of Beclin1 in multiple system atrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Huanzhu Liu,Ruoyang Yu,Muwei Zhang,Xiaoyan Zheng,Lizi Zhong,Wanlin Yang,Yuqi Luo,Zifeng Huang,Jialing Zheng,Hui Zhong,Xiaobo Wei,Wenhua Zheng,Yinghua Yu,Qing Wang

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 43:241-257 PubMed36457151

2022

Neuronal Serpina3n is an endogenous protector against blood brain barrier damage following cerebral ischemic stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Fengshi Li,Yueman Zhang,Ruqi Li,Yan Li,Shenghao Ding,Jianpo Zhou,Tianchen Huang,Chen Chen,Bingwei Lu,Weifeng Yu,Johannes Boltze,Peiying Li,Jieqing Wan

Nature cardiovascular research 1:1195-1214 PubMed39196168

2022

Insulin-like growth factor-binding protein-7 (IGFBP7) links senescence to heart failure.

Applications

Unspecified application

Species

Unspecified reactive species

Liyong Zhang,David Smyth,Mohammad Al-Khalaf,Alice Blet,Qiujiang Du,Jordan Bernick,Michael Gong,Xu Chi,Yena Oh,Malaika Roba-Oshin,Elizabeth Coletta,Michel Feletou,Anthony O Gramolini,Kyoung-Han Kim,Thais Coutinho,James L Januzzi,Benoit Tyl,Andre Ziegler,Peter P Liu

Electrophoresis 43:1784-1798 PubMed35753078

2022

cyc-DEP: Cyclic immunofluorescence profiling of particles collected using dielectrophoresis.

Applications

Unspecified application

Species

Unspecified reactive species

Kyle T Gustafson,Zeynep Sayar,Hillary Le,Steven L Gustafson,Austin Gower,Augusta Modestino,Stuart Ibsen,Michael J Heller,Sadik Esener,Sebnem E Eksi

Journal of personalized medicine 10: PubMed33096722

2020

Why Does COVID-19 Affect Patients with Spinal Cord Injury Milder? A Case-Control Study: Results from Two Observational Cohorts.

Applications

Unspecified application

Species

Unspecified reactive species

Enrique Calvo,Nerea Corbacho-Alonso,Tamara Sastre-Oliva,Estefania Nuñez,Patricia Baena-Galan,German Hernandez-Fernandez,Miguel Rodriguez-Cola,Irena Jimenez-Velasco,Fernando J Corrales,Claudia Gambarrutta-Malfati,Francisco Gutierrez-Henares,Elisa Lopez-Dolado,Angel Gil-Agudo,Jesus Vazquez,Laura Mourino-Alvarez,Maria G Barderas

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Fibrinogen beta chain antibody [EPR19765]

Immunocytochemistry/ Immunofluorescence - Anti-Fibrinogen beta chain antibody [EPR19765] (AB193932)

Immunoprecipitation - Anti-Fibrinogen beta chain antibody [EPR19765] (AB193932)

Western blot - Anti-Fibrinogen beta chain antibody [EPR19765] (AB193932)

Western blot - Anti-Fibrinogen beta chain antibody [EPR19765] (AB193932)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (6)

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