AB251177

Anti-Fibrinogen beta chain antibody [EPR19765] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal Fibrinogen beta chain antibody. Carrier free. Suitable for ICC/IF, IP, WB and reacts with Human samples.

View Alternative Names

Fibrinogen beta chain, FGB

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-Fibrinogen beta chain antibody [EPR19765] - BSA and Azide free (AB251177)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Fibrinogen beta chain antibody [EPR19765] - BSA and Azide free (AB251177)

This data was developed using ab193932, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% PFA-fixed, 0.1% Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells labeling Fibrinogen beta chain with ab193932 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining of Fibrinogen beta on HepG2 cells.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

Supplier Data

Immunoprecipitation - Anti-Fibrinogen beta chain antibody [EPR19765] - BSA and Azide free (AB251177)

This data was developed using ab193932, the same antibody clone in a different buffer formulation.

Fibrinogen beta chain was immunoprecipitated from 0.35 mg of HepG2 (human liver hepatocellular carcinoma cell line) lysate with ab193932 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab193932 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Lane 1 : HepG2 whole cell lysate 10 μg (Input).

Lane 2 : ab193932 IP in HepG2 whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab193932 in HepG2 whole cell lysate.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 30 seconds.

All lanes:

Immunoprecipitation - Anti-Fibrinogen beta chain antibody [EPR19765] (<a href='/en-us/products/primary-antibodies/fibrinogen-beta-chain-antibody-epr19765-ab193932'>ab193932</a>)

Predicted band size: 56 kDa

Observed band size: 55 kDa

true

Supplier Data

Western blot - Anti-Fibrinogen beta chain antibody [EPR19765] - BSA and Azide free (AB251177)

This data was developed using ab193932, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1 : 5 seconds; Lane 2 : 1 second; Lane 3 : 3 minutes.

All lanes:

Western blot - Anti-Fibrinogen beta chain antibody [EPR19765] (<a href='/en-us/products/primary-antibodies/fibrinogen-beta-chain-antibody-epr19765-ab193932'>ab193932</a>) at 1/1000 dilution

Lane 1:

Human fetal liver tissue lysate at 20 µg

Lane 2:

Human plasma at 20 µg

Lane 3:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg

Secondary

Lanes 1 - 2:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Lane 3:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 56 kDa

Observed band size: 55 kDa

true

Supplier Data

Western blot - Anti-Fibrinogen beta chain antibody [EPR19765] - BSA and Azide free (AB251177)

This data was developed using ab193932, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Fibrinogen beta chain antibody [EPR19765] (<a href='/en-us/products/primary-antibodies/fibrinogen-beta-chain-antibody-epr19765-ab193932'>ab193932</a>) at 1/2000 dilution

Lane 1:

His-tagged human fibrinogen alpha chain recombinant protein (aa401-866) at 0.02 µg

Lane 2:

GST-His-tagged human fibrinogen beta chain recombinant protein (aa1-491) at 0.02 µg

Lane 3:

GST-His-tagged human fibrinogen gamma chain recombinant protein (aa1-453) at 0.02 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 56 kDa

Observed band size: 80 kDa

true

Exposure time: 1s

Unconjugated

Anti-Fibrinogen beta chain antibody [EPR19765]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19765

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

ab251177 is the carrier-free version of ab193932.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cleaved by the protease thrombin to yield monomers which, together with fibrinogen alpha (FGA) and fibrinogen gamma (FGG), polymerize to form an insoluble fibrin matrix. Fibrin has a major function in hemostasis as one of the primary components of blood clots. In addition, functions during the early stages of wound repair to stabilize the lesion and guide cell migration during re-epithelialization. Was originally thought to be essential for platelet aggregation, based on in vitro studies using anticoagulated blood. However subsequent studies have shown that it is not absolutely required for thrombus formation in vivo. Enhances expression of SELP in activated platelets. Maternal fibrinogen is essential for successful pregnancy. Fibrin deposition is also associated with infection, where it protects against IFNG-mediated hemorrhage. May also facilitate the antibacterial immune response via both innate and T-cell mediated pathways.

See full target information Fibrinogen beta chain

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Fibrinogen beta chain antibody [EPR19765] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-Fibrinogen beta chain antibody [EPR19765] - BSA and Azide free (AB251177)

Immunoprecipitation - Anti-Fibrinogen beta chain antibody [EPR19765] - BSA and Azide free (AB251177)

Western blot - Anti-Fibrinogen beta chain antibody [EPR19765] - BSA and Azide free (AB251177)

Western blot - Anti-Fibrinogen beta chain antibody [EPR19765] - BSA and Azide free (AB251177)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Product promise