Rabbit Recombinant Monoclonal Fibrinogen beta chain antibody. Carrier free. Suitable for ICC/IF, IP, WB and reacts with Human samples.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-Fibrinogen beta chain antibody [EPR19765] - BSA and Azide free (AB251177), expandable thumbnail](https://content.abcam.com/products/images/fibrinogen-beta-chain-antibody-epr19765-bsa-and-azide-free-ab251177--immunocytochemistry-immunofluorescence-img133134.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Fibrinogen beta chain antibody [EPR19765] - BSA and Azide free (AB251177)")
![Western blot - Anti-Fibrinogen beta chain antibody [EPR19765] - BSA and Azide free (AB251177), expandable thumbnail](https://content.abcam.com/products/images/fibrinogen-beta-chain-antibody-epr19765-bsa-and-azide-free-ab251177--western-blot-img9271.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Fibrinogen beta chain antibody [EPR19765] - BSA and Azide free (AB251177)")
![Immunoprecipitation - Anti-Fibrinogen beta chain antibody [EPR19765] - BSA and Azide free (AB251177), expandable thumbnail](https://content.abcam.com/products/images/fibrinogen-beta-chain-antibody-epr19765-bsa-and-azide-free-ab251177--immunoprecipitation-img3803.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Fibrinogen beta chain antibody [EPR19765] - BSA and Azide free (AB251177)")
![Western blot - Anti-Fibrinogen beta chain antibody [EPR19765] - BSA and Azide free (AB251177), expandable thumbnail](https://content.abcam.com/products/images/fibrinogen-beta-chain-antibody-epr19765-bsa-and-azide-free-ab251177--western-blot-img147654.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Fibrinogen beta chain antibody [EPR19765] - BSA and Azide free (AB251177)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | WB | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 products for Alternative Version
Target data
Function
Cleaved by the protease thrombin to yield monomers which, together with fibrinogen alpha (FGA) and fibrinogen gamma (FGG), polymerize to form an insoluble fibrin matrix. Fibrin has a major function in hemostasis as one of the primary components of blood clots. In addition, functions during the early stages of wound repair to stabilize the lesion and guide cell migration during re-epithelialization. Was originally thought to be essential for platelet aggregation, based on in vitro studies using anticoagulated blood. However subsequent studies have shown that it is not absolutely required for thrombus formation in vivo. Enhances expression of SELP in activated platelets. Maternal fibrinogen is essential for successful pregnancy. Fibrin deposition is also associated with infection, where it protects against IFNG-mediated hemorrhage. May also facilitate the antibacterial immune response via both innate and T-cell mediated pathways.
Alternative names
Fibrinogen beta chain, FGB
Recommended products
Rabbit Recombinant Monoclonal Fibrinogen beta chain antibody. Carrier free. Suitable for ICC/IF, IP, WB and reacts with Human samples.
Alternative names
Fibrinogen beta chain, FGB
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR19765
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab251177 is the carrier-free version of Anti-Fibrinogen beta chain antibody [EPR19765] ab193932.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunocytochemistry/ Immunofluorescence - Anti-Fibrinogen beta chain antibody [EPR19765] - BSA and Azide free (ab251177), expandable thumbnail](https://content.abcam.com/products/images/fibrinogen-beta-chain-antibody-epr19765-bsa-and-azide-free-ab251177--immunocytochemistry-immunofluorescence-img133134.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Fibrinogen beta chain antibody [EPR19765] - BSA and Azide free (ab251177)")
Immunocytochemistry/ Immunofluorescence - Anti-Fibrinogen beta chain antibody [EPR19765] - BSA and Azide free (ab251177)
This data was developed using Anti-Fibrinogen beta chain antibody [EPR19765] ab193932, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% PFA-fixed, 0.1% Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells labeling Fibrinogen beta chain with Anti-Fibrinogen beta chain antibody [EPR19765] ab193932 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining of Fibrinogen beta on HepG2 cells.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) (red) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.
![Western blot - Anti-Fibrinogen beta chain antibody [EPR19765] - BSA and Azide free (ab251177), expandable thumbnail](https://content.abcam.com/products/images/fibrinogen-beta-chain-antibody-epr19765-bsa-and-azide-free-ab251177--western-blot-img9271.jpg "Western blot - Anti-Fibrinogen beta chain antibody [EPR19765] - BSA and Azide free (ab251177)")
Western blot - Anti-Fibrinogen beta chain antibody [EPR19765] - BSA and Azide free (ab251177)
This data was developed using Anti-Fibrinogen beta chain antibody [EPR19765] ab193932, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Fibrinogen beta chain antibody [EPR19765] (Anti-Fibrinogen beta chain antibody [EPR19765] ab193932) at 1/2000 dilution
Lane 1: His-tagged human fibrinogen alpha chain recombinant protein (aa401-866) at 0.02 µg
Lane 2: GST-His-tagged human fibrinogen beta chain recombinant protein (aa1-491) at 0.02 µg
Lane 3: GST-His-tagged human fibrinogen gamma chain recombinant protein (aa1-453) at 0.02 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 56 kDa
Observed band size: 80 kDa
Exposure time: 1s
Blocking/Dilution buffer: 5% NFDM/TBST.
![Immunoprecipitation - Anti-Fibrinogen beta chain antibody [EPR19765] - BSA and Azide free (ab251177), expandable thumbnail](https://content.abcam.com/products/images/fibrinogen-beta-chain-antibody-epr19765-bsa-and-azide-free-ab251177--immunoprecipitation-img3803.jpg "Immunoprecipitation - Anti-Fibrinogen beta chain antibody [EPR19765] - BSA and Azide free (ab251177)")
Immunoprecipitation - Anti-Fibrinogen beta chain antibody [EPR19765] - BSA and Azide free (ab251177)
This data was developed using Anti-Fibrinogen beta chain antibody [EPR19765] ab193932, the same antibody clone in a different buffer formulation.
Fibrinogen beta chain was immunoprecipitated from 0.35 mg of HepG2 (human liver hepatocellular carcinoma cell line) lysate with Anti-Fibrinogen beta chain antibody [EPR19765] ab193932 at 1/30 dilution. Western blot was performed from the immunoprecipitate using Anti-Fibrinogen beta chain antibody [EPR19765] ab193932 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/1000 dilution.
Lane 1: HepG2 whole cell lysate 10 μg (Input).
Lane 2: Anti-Fibrinogen beta chain antibody [EPR19765] ab193932 IP in HepG2 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Fibrinogen beta chain antibody [EPR19765] ab193932 in HepG2 whole cell lysate.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 30 seconds.
All lanes: Immunoprecipitation - Anti-Fibrinogen beta chain antibody [EPR19765] (Anti-Fibrinogen beta chain antibody [EPR19765] ab193932)
Developed using the ECL technique.
Predicted band size: 56 kDa
Observed band size: 55 kDa
![Western blot - Anti-Fibrinogen beta chain antibody [EPR19765] - BSA and Azide free (ab251177), expandable thumbnail](https://content.abcam.com/products/images/fibrinogen-beta-chain-antibody-epr19765-bsa-and-azide-free-ab251177--western-blot-img147654.jpg "Western blot - Anti-Fibrinogen beta chain antibody [EPR19765] - BSA and Azide free (ab251177)")
Western blot - Anti-Fibrinogen beta chain antibody [EPR19765] - BSA and Azide free (ab251177)
This data was developed using Anti-Fibrinogen beta chain antibody [EPR19765] ab193932, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 5 seconds; Lane 2: 1 second; Lane 3: 3 minutes.
All lanes: Western blot - Anti-Fibrinogen beta chain antibody [EPR19765] (Anti-Fibrinogen beta chain antibody [EPR19765] ab193932) at 1/1000 dilution
Lane 1: Human fetal liver tissue lysate at 20 µg
Lane 2: Human plasma at 20 µg
Lane 3: HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg
SecondaryLanes 1 - 2: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution
Lane 3: Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 56 kDa
Observed band size: 55 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com