AB96532

Anti-Fibrinogen gamma chain antibody

Name: Anti-Fibrinogen gamma chain antibody (ab96532) | Abcam
Brand: Abcam Limited
SKU: AB96532

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(5 Publications)

Rabbit Polyclonal Fibrinogen gamma chain antibody. Suitable for IP, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human FGG aa 1 to C-terminus.

View Alternative Names

PRO2061, FGG, Fibrinogen gamma chain

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-Fibrinogen gamma chain antibody (AB96532)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Fibrinogen gamma chain antibody (AB96532)

Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling Fibrinogen gamma protein using ab96532 at a 1/500 dilution (Green). alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody at a 1/10000 dilution (Red). Hoechst 33342 was used for nuclear staining (Blue).

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Fibrinogen gamma chain antibody (AB96532)

Immunofluorescence analysis of HepG2 cells fixed in 4% paraformaldehyde at RT for 15 min labelling Fibrinogen gamma protein using ab96532 at a 1/500 dilution (Green). DAPI was used for nuclear staining (Blue). Scale bar= 10µm.

Supplier Data

Western blot - Anti-Fibrinogen gamma chain antibody (AB96532)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-Fibrinogen gamma chain antibody (ab96532) at 1/1000 dilution

All lanes:

Mouse liver tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 50 kDa

false

Supplier Data

Western blot - Anti-Fibrinogen gamma chain antibody (AB96532)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-Fibrinogen gamma chain antibody (ab96532) at 1/500 dilution

All lanes:

Rat plasma at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 50 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IP, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human FGG aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P02679

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/20000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/20000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/20000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Together with fibrinogen alpha (FGA) and fibrinogen beta (FGB), polymerizes to form an insoluble fibrin matrix. Has a major function in hemostasis as one of the primary components of blood clots. In addition, functions during the early stages of wound repair to stabilize the lesion and guide cell migration during re-epithelialization. Was originally thought to be essential for platelet aggregation, based on in vitro studies using anticoagulated blood. However, subsequent studies have shown that it is not absolutely required for thrombus formation in vivo. Enhances expression of SELP in activated platelets via an ITGB3-dependent pathway. Maternal fibrinogen is essential for successful pregnancy. Fibrin deposition is also associated with infection, where it protects against IFNG-mediated hemorrhage. May also facilitate the antibacterial immune response via both innate and T-cell mediated pathways.

See full target information FGG

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:8430 PubMed39341842

2024

The urotensin II receptor triggers an early meningeal response and a delayed macrophage-dependent vasospasm after subarachnoid hemorrhage in male mice.

Applications

Unspecified application

Species

Unspecified reactive species

Martin Pedard,Lucie Prevost,Camille Carpena,Brian Holleran,Laurence Desrues,Martine Dubois,Celeste Nicola,Roxane Gruel,David Godefroy,Thomas Deffieux,Mickael Tanter,Carine Ali,Richard Leduc,Laurent Prézeau,Pierrick Gandolfo,Fabrice Morin,Olivier Wurtz,Thomas Bonnard,Denis Vivien,Hélène Castel

Research and practice in thrombosis and haemostasis 6:e12697 PubMed35494504

2022

Factor XIII is a newly identified binding partner for platelet collagen receptor GPVI-dimer-An interaction that may modulate fibrin crosslinking.

Applications

Unspecified application

Species

Unspecified reactive species

Masaaki Moroi,Isuru Induruwa,Richard W Farndale,Stephanie M Jung

Experimental neurobiology 28:104-118 PubMed30853828

2019

Persistent Malfunction of Glymphatic and Meningeal Lymphatic Drainage in a Mouse Model of Subarachnoid Hemorrhage.

Applications

Unspecified application

Species

Unspecified reactive species

Tinglin Pu,Wenyan Zou,Weixi Feng,Yanli Zhang,Linmei Wang,Hongxing Wang,Ming Xiao

Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics 15:216-232 PubMed29247448

2017

iTRAQ-Based Quantitative Proteomics Reveals the New Evidence Base for Traumatic Brain Injury Treated with Targeted Temperature Management.

Applications

Unspecified application

Species

Unspecified reactive species

Shi-Xiang Cheng,Zhong-Wei Xu,Tai-Long Yi,Hong-Tao Sun,Cheng Yang,Ze-Qi Yu,Xiao-Sa Yang,Xiao-Han Jin,Yue Tu,Sai Zhang

The Journal of biological chemistry 292:9063-9074 PubMed28364042

2017

Kinetic-based trapping by intervening sequence variants of the active sites of protein-disulfide isomerase identifies platelet protein substrates.

Applications

Unspecified application

Species

Unspecified reactive species

Jack D Stopa,Katherine M Baker,Steven P Grover,Robert Flaumenhaft,Bruce Furie

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Fibrinogen gamma chain antibody

Immunocytochemistry/ Immunofluorescence - Anti-Fibrinogen gamma chain antibody (AB96532)

Immunocytochemistry/ Immunofluorescence - Anti-Fibrinogen gamma chain antibody (AB96532)

Western blot - Anti-Fibrinogen gamma chain antibody (AB96532)

Western blot - Anti-Fibrinogen gamma chain antibody (AB96532)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (5)

Product promise