Rabbit Recombinant Monoclonal Fibrinogen gamma chain antibody. Suitable for ICC/IF, IP, WB, IHC-P and reacts with Human, Rat, Mouse samples. Cited in 2 publications.
Images
![Western blot - Anti-Fibrinogen gamma chain antibody [EP24539-127] (AB281924), expandable thumbnail](https://content.abcam.com/products/images/fibrinogen-gamma-chain-antibody-ep24539-127-ab281924--western-blot-img174784.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Fibrinogen gamma chain antibody [EP24539-127] (AB281924)")
![Western blot - Anti-Fibrinogen gamma chain antibody [EP24539-127] (AB281924), expandable thumbnail](https://content.abcam.com/products/images/fibrinogen-gamma-chain-antibody-ep24539-127-ab281924--western-blot-img180088.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Fibrinogen gamma chain antibody [EP24539-127] (AB281924)")
![Western blot - Anti-Fibrinogen gamma chain antibody [EP24539-127] (AB281924), expandable thumbnail](https://content.abcam.com/products/images/fibrinogen-gamma-chain-antibody-ep24539-127-ab281924--western-blot-img175982.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Fibrinogen gamma chain antibody [EP24539-127] (AB281924)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen gamma chain antibody [EP24539-127] (AB281924), expandable thumbnail](https://content.abcam.com/products/images/fibrinogen-gamma-chain-antibody-ep24539-127-ab281924--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img171000.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen gamma chain antibody [EP24539-127] (AB281924)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen gamma chain antibody [EP24539-127] (AB281924), expandable thumbnail](https://content.abcam.com/products/images/fibrinogen-gamma-chain-antibody-ep24539-127-ab281924--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img167065.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen gamma chain antibody [EP24539-127] (AB281924)")
Publications
Filter by
- American journal of cancer research 12:5552-55632022Claudin 19 inhibits the malignant potential of breast cancer cells by modulating extracellular matrix-associated UBE2C/Wnt signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJingxiu Xu,Mingjie Chen,Mingyu Hu,Hanlu Wang,Zhongqiang Zuo,Jianguo Wang,Zhiyin XiePubMed 36628278
- Frontiers in physiology 12:7312342021Revealing the Angiopathy of Lacrimal Gland Lesion in Type 2 Diabetes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJunfa Xue,Bin Zhang,Shengqian Dou,Qingjun Zhou,Min Ding,Mingming Zhou,Huifeng Wang,Yanling Dong,Dongfang Li,Lixin XiePubMed 34531764
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | Flow Cyt | WB | IHC-P | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Tested | Tested |
| Mouse | Not recommended | Tested | Not recommended | Tested | Not recommended |
| Rat | Expected | Expected | Not recommended | Tested | Not recommended |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/30 | Notes - |
Species Human | Dilution info 1/30 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/4000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Together with fibrinogen alpha (FGA) and fibrinogen beta (FGB), polymerizes to form an insoluble fibrin matrix. Has a major function in hemostasis as one of the primary components of blood clots. In addition, functions during the early stages of wound repair to stabilize the lesion and guide cell migration during re-epithelialization. Was originally thought to be essential for platelet aggregation, based on in vitro studies using anticoagulated blood. However, subsequent studies have shown that it is not absolutely required for thrombus formation in vivo. Enhances expression of SELP in activated platelets via an ITGB3-dependent pathway. Maternal fibrinogen is essential for successful pregnancy. Fibrin deposition is also associated with infection, where it protects against IFNG-mediated hemorrhage. May also facilitate the antibacterial immune response via both innate and T-cell mediated pathways.
Alternative names
PRO2061, FGG, Fibrinogen gamma chain
Recommended products
Rabbit Recombinant Monoclonal Fibrinogen gamma chain antibody. Suitable for ICC/IF, IP, WB, IHC-P and reacts with Human, Rat, Mouse samples. Cited in 2 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EP24539-127
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
9 product images
![Western blot - Anti-Fibrinogen gamma chain antibody [EP24539-127] (ab281924), expandable thumbnail](https://content.abcam.com/products/images/fibrinogen-gamma-chain-antibody-ep24539-127-ab281924--western-blot-img174784.jpg "Western blot - Anti-Fibrinogen gamma chain antibody [EP24539-127] (ab281924)")
Western blot - Anti-Fibrinogen gamma chain antibody [EP24539-127] (ab281924)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 21480334).
Exposure time: Lanes 1-5: 10 seconds
Lane 6: 37 seconds.
All lanes: Western blot - Anti-Fibrinogen gamma chain antibody [EP24539-127] (ab281924) at 1/5000 dilution
Lane 1: Human plasma lysate at 10 µg
Lane 2: Human plasma lysate treated with PNGase F whole cell lysate at 10 µg
Lane 3: Mouse plasma lysate at 10 µg
Lane 4: Mouse plasma lysate treated with PNGase F whole cell lysate at 10 µg
Lane 5: Rat plasma lysate at 10 µg
Lane 6: Rat liver lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/20000 dilution
Predicted band size: 52 kDa
Observed band size: 100 kDa, 50 kDa
![Western blot - Anti-Fibrinogen gamma chain antibody [EP24539-127] (ab281924), expandable thumbnail](https://content.abcam.com/products/images/fibrinogen-gamma-chain-antibody-ep24539-127-ab281924--western-blot-img180088.jpg "Western blot - Anti-Fibrinogen gamma chain antibody [EP24539-127] (ab281924)")
Western blot - Anti-Fibrinogen gamma chain antibody [EP24539-127] (ab281924)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Low expression: A549/DU145 (PMID: 17949478).
All lanes: Western blot - Anti-Fibrinogen gamma chain antibody [EP24539-127] (ab281924) at 1/1000 dilution
Lane 1: HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate at 10 µg
Lane 2: A549 (human lung carcinoma epithelial cell), whole cell lysate at 10 µg
Lane 3: DU145 (human prostate carcinoma epithelial cell), whole cell lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Predicted band size: 52 kDa
Observed band size: 50 kDa
Exposure time: 26s
![Western blot - Anti-Fibrinogen gamma chain antibody [EP24539-127] (ab281924), expandable thumbnail](https://content.abcam.com/products/images/fibrinogen-gamma-chain-antibody-ep24539-127-ab281924--western-blot-img175982.jpg "Western blot - Anti-Fibrinogen gamma chain antibody [EP24539-127] (ab281924)")
Western blot - Anti-Fibrinogen gamma chain antibody [EP24539-127] (ab281924)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: Lanes 1: 1 seconds.
Lane 2: 26 seconds.
All lanes: Western blot - Anti-Fibrinogen gamma chain antibody [EP24539-127] (ab281924) at 1/1000 dilution
Lane 1: Human liver lysate at 10 µg
Lane 2: Mouse liver lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/1000 dilution
Predicted band size: 52 kDa
Observed band size: 100 kDa, 50 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen gamma chain antibody [EP24539-127] (ab281924), expandable thumbnail](https://content.abcam.com/products/images/fibrinogen-gamma-chain-antibody-ep24539-127-ab281924--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img171000.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen gamma chain antibody [EP24539-127] (ab281924)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen gamma chain antibody [EP24539-127] (ab281924)
Immunohistochemical analysis of paraffin-embedded Human liver tissue labelling Fibrinogen gamma chain with ab281924 at 1/4000 (0.135 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human liver (PMID:24648543). The section was incubated with ab281924 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen gamma chain antibody [EP24539-127] (ab281924), expandable thumbnail](https://content.abcam.com/products/images/fibrinogen-gamma-chain-antibody-ep24539-127-ab281924--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img167065.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen gamma chain antibody [EP24539-127] (ab281924)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen gamma chain antibody [EP24539-127] (ab281924)
Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labelling Fibrinogen gamma chain with ab281924 at 1/4000 (0.135 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining in the blood plasma and stroma of human pancreas (PMID:16510577). The section was incubated with ab281924 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Immunoprecipitation - Anti-Fibrinogen gamma chain antibody [EP24539-127] (ab281924), expandable thumbnail](https://content.abcam.com/products/images/fibrinogen-gamma-chain-antibody-ep24539-127-ab281924--immunoprecipitation-img175783.jpg "Immunoprecipitation - Anti-Fibrinogen gamma chain antibody [EP24539-127] (ab281924)")
Immunoprecipitation - Anti-Fibrinogen gamma chain antibody [EP24539-127] (ab281924)
Fibrinogen gamma chain was immunoprecipitated from 0.35 mg HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate 10 ug with ab281924 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab281924 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate 10 ug.
Lane 2: ab281924 IP in HepG2 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab281924 in HepG2 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.
All lanes: Immunoprecipitation - Anti-Fibrinogen gamma chain antibody [EP24539-127] (ab281924)
Predicted band size: 52 kDa
Observed band size: 50 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen gamma chain antibody [EP24539-127] (ab281924), expandable thumbnail](https://content.abcam.com/products/images/fibrinogen-gamma-chain-antibody-ep24539-127-ab281924--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img167026.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen gamma chain antibody [EP24539-127] (ab281924)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen gamma chain antibody [EP24539-127] (ab281924)
Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labelling Fibrinogen gamma chain with ab281924 at 1/4000 (0.135 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human hepatocellular carcinoma (PMID:30863175). The section was incubated with ab281924 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Immunoprecipitation - Anti-Fibrinogen gamma chain antibody [EP24539-127] (ab281924), expandable thumbnail](https://content.abcam.com/products/images/fibrinogen-gamma-chain-antibody-ep24539-127-ab281924--immunoprecipitation-img182544.jpg "Immunoprecipitation - Anti-Fibrinogen gamma chain antibody [EP24539-127] (ab281924)")
Immunoprecipitation - Anti-Fibrinogen gamma chain antibody [EP24539-127] (ab281924)
Fibrinogen gamma chain was immunoprecipitated from 0.35 mg Mouse plasma lysate 10 ug with ab281924 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab281924 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Mouse plasma lysate 10 ug.
Lane 2: ab281924 IP in Mouse plasma lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab281924 in mouse plasma lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.
All lanes: Immunoprecipitation - Anti-Fibrinogen gamma chain antibody [EP24539-127] (ab281924)
Predicted band size: 52 kDa
Observed band size: 50 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-Fibrinogen gamma chain antibody [EP24539-127] (ab281924), expandable thumbnail](https://content.abcam.com/products/images/fibrinogen-gamma-chain-antibody-ep24539-127-ab281924--immunocytochemistry-immunofluorescence-img166189.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Fibrinogen gamma chain antibody [EP24539-127] (ab281924)")
Immunocytochemistry/ Immunofluorescence - Anti-Fibrinogen gamma chain antibody [EP24539-127] (ab281924)
Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized HepG2 cells labelling Fibrinogen gamma chain with ab281924 at 1/100 (5.38 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green) Confocal image showing cytoplasmic staining in HepG2 cells is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
![Immunoprecipitation - Anti-Fibrinogen gamma chain antibody [EP24539-127] (ab281924), expandable thumbnail](https://content.abcam.com/products/images/fibrinogen-gamma-chain-antibody-ep24539-127-ab281924--immunoprecipitation-img175783.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Fibrinogen gamma chain antibody [EP24539-127] (ab281924)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen gamma chain antibody [EP24539-127] (ab281924), expandable thumbnail](https://content.abcam.com/products/images/fibrinogen-gamma-chain-antibody-ep24539-127-ab281924--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img167026.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen gamma chain antibody [EP24539-127] (ab281924)")
![Immunoprecipitation - Anti-Fibrinogen gamma chain antibody [EP24539-127] (ab281924), expandable thumbnail](https://content.abcam.com/products/images/fibrinogen-gamma-chain-antibody-ep24539-127-ab281924--immunoprecipitation-img182544.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Fibrinogen gamma chain antibody [EP24539-127] (ab281924)")
![Immunocytochemistry/ Immunofluorescence - Anti-Fibrinogen gamma chain antibody [EP24539-127] (ab281924), expandable thumbnail](https://content.abcam.com/products/images/fibrinogen-gamma-chain-antibody-ep24539-127-ab281924--immunocytochemistry-immunofluorescence-img166189.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Fibrinogen gamma chain antibody [EP24539-127] (ab281924)")
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