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AB281944

Anti-Fibrinogen gamma chain antibody [EP24539-127] - BSA and Azide free

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Rabbit Recombinant Monoclonal Fibrinogen gamma chain antibody. Carrier free. Suitable for ICC/IF, IP, WB, IHC-P and reacts with Human, Rat, Mouse samples.

View Alternative Names

PRO2061, FGG, Fibrinogen gamma chain

9 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen gamma chain antibody [EP24539-127] - BSA and Azide free (AB281944)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen gamma chain antibody [EP24539-127] - BSA and Azide free (AB281944)

This data was developed using ab281924, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labelling Fibrinogen gamma chain with ab281924 at 1/4000 (0.135 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining in the blood plasma and stroma of human pancreas (PMID : 16510577). The section was incubated with ab281924 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen gamma chain antibody [EP24539-127] - BSA and Azide free (AB281944)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen gamma chain antibody [EP24539-127] - BSA and Azide free (AB281944)

This data was developed using ab281924, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labelling Fibrinogen gamma chain with ab281924 at 1/4000 (0.135 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human hepatocellular carcinoma (PMID : 30863175). The section was incubated with ab281924 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Immunocytochemistry/ Immunofluorescence - Anti-Fibrinogen gamma chain antibody [EP24539-127] - BSA and Azide free (AB281944)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Fibrinogen gamma chain antibody [EP24539-127] - BSA and Azide free (AB281944)

This data was developed using ab281924, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized HepG2 cells labelling Fibrinogen gamma chain with ab281924 at 1/100 (5.38 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green) Confocal image showing cytoplasmic staining in HepG2 cells is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen gamma chain antibody [EP24539-127] - BSA and Azide free (AB281944)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen gamma chain antibody [EP24539-127] - BSA and Azide free (AB281944)

This data was developed using ab281924, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human liver tissue labelling Fibrinogen gamma chain with ab281924 at 1/4000 (0.135 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human liver (PMID : 24648543). The section was incubated with ab281924 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Immunoprecipitation - Anti-Fibrinogen gamma chain antibody [EP24539-127] - BSA and Azide free (AB281944)
  • IP

Supplier Data

Immunoprecipitation - Anti-Fibrinogen gamma chain antibody [EP24539-127] - BSA and Azide free (AB281944)

This data was developed using ab281924, the same antibody clone in a different buffer formulation.

Fibrinogen gamma chain was immunoprecipitated from 0.35 mg HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate 10 ug with ab281924 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab281924 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate 10 ug

Lane 2 : ab281924 IP in HepG2 whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab281924 in HepG2 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 seconds.

All lanes:

Immunoprecipitation - Anti-Fibrinogen gamma chain antibody [EP24539-127] (<a href='/en-us/products/primary-antibodies/fibrinogen-gamma-chain-antibody-ep24539-127-ab281924'>ab281924</a>)

Predicted band size: 52 kDa

Observed band size: 50 kDa

false

Immunoprecipitation - Anti-Fibrinogen gamma chain antibody [EP24539-127] - BSA and Azide free (AB281944)
  • IP

Supplier Data

Immunoprecipitation - Anti-Fibrinogen gamma chain antibody [EP24539-127] - BSA and Azide free (AB281944)

This data was developed using ab281924, the same antibody clone in a different buffer formulation.

Fibrinogen gamma chain was immunoprecipitated from 0.35 mg Mouse plasma lysate 10 ug with ab281924 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab281924 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : Mouse plasma lysate 10 ug

Lane 2 : ab281924 IP in Mouse plasma lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab281924 in mouse plasma lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 seconds.

All lanes:

Immunoprecipitation - Anti-Fibrinogen gamma chain antibody [EP24539-127] (<a href='/en-us/products/primary-antibodies/fibrinogen-gamma-chain-antibody-ep24539-127-ab281924'>ab281924</a>)

Predicted band size: 52 kDa

Observed band size: 50 kDa

false

Western blot - Anti-Fibrinogen gamma chain antibody [EP24539-127] - BSA and Azide free (AB281944)
  • WB

Lab

Western blot - Anti-Fibrinogen gamma chain antibody [EP24539-127] - BSA and Azide free (AB281944)

This data was developed using ab281924, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Exposure time : Lanes 1 : 1.

seconds Lane 2 : 26 seconds.

All lanes:

Western blot - Anti-Fibrinogen gamma chain antibody [EP24539-127] (<a href='/en-us/products/primary-antibodies/fibrinogen-gamma-chain-antibody-ep24539-127-ab281924'>ab281924</a>) at 1/1000 dilution

Lane 1:

Human liver lysate at 10 µg

Lane 2:

Mouse liver lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/1000 dilution

Predicted band size: 52 kDa

Observed band size: 100 kDa,50 kDa

false

Western blot - Anti-Fibrinogen gamma chain antibody [EP24539-127] - BSA and Azide free (AB281944)
  • WB

Lab

Western blot - Anti-Fibrinogen gamma chain antibody [EP24539-127] - BSA and Azide free (AB281944)

This data was developed using ab281924, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Low expression : A549/DU145 (PMID : 17949478).

All lanes:

Western blot - Anti-Fibrinogen gamma chain antibody [EP24539-127] (<a href='/en-us/products/primary-antibodies/fibrinogen-gamma-chain-antibody-ep24539-127-ab281924'>ab281924</a>) at 1/1000 dilution

Lane 1:

HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate at 10 µg

Lane 2:

A549 (human lung carcinoma epithelial cell), whole cell lysate at 10 µg

Lane 3:

DU145 (human prostate carcinoma epithelial cell), whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 52 kDa

Observed band size: 50 kDa

false

Exposure time: 26s

Western blot - Anti-Fibrinogen gamma chain antibody [EP24539-127] - BSA and Azide free (AB281944)
  • WB

Lab

Western blot - Anti-Fibrinogen gamma chain antibody [EP24539-127] - BSA and Azide free (AB281944)

This data was developed using ab281924, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 21480334).

Exposure time : Lanes 1-5 : 10 seconds.

Lane 6 : 37 seconds.

All lanes:

Western blot - Anti-Fibrinogen gamma chain antibody [EP24539-127] (<a href='/en-us/products/primary-antibodies/fibrinogen-gamma-chain-antibody-ep24539-127-ab281924'>ab281924</a>) at 1/5000 dilution

Lane 1:

Human plasma lysate at 10 µg

Lane 2:

Human plasma lysate treated with PNGase F whole cell lysate at 10 µg

Lane 3:

Mouse plasma lysate at 10 µg

Lane 4:

Mouse plasma lysate treated with PNGase F whole cell lysate at 10 µg

Lane 5:

Rat plasma lysate at 10 µg

Lane 6:

Rat liver lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/20000 dilution

Predicted band size: 52 kDa

Observed band size: 100 kDa,50 kDa

false

  • Unconjugated

    Anti-Fibrinogen gamma chain antibody [EP24539-127]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP24539-127

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

IP, IHC-P, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Secondary Antibodies

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Reactivity data

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Product details

ab281944 is the carrier-free version of ab281924.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Together with fibrinogen alpha (FGA) and fibrinogen beta (FGB), polymerizes to form an insoluble fibrin matrix. Has a major function in hemostasis as one of the primary components of blood clots. In addition, functions during the early stages of wound repair to stabilize the lesion and guide cell migration during re-epithelialization. Was originally thought to be essential for platelet aggregation, based on in vitro studies using anticoagulated blood. However, subsequent studies have shown that it is not absolutely required for thrombus formation in vivo. Enhances expression of SELP in activated platelets via an ITGB3-dependent pathway. Maternal fibrinogen is essential for successful pregnancy. Fibrin deposition is also associated with infection, where it protects against IFNG-mediated hemorrhage. May also facilitate the antibacterial immune response via both innate and T-cell mediated pathways.
See full target information FGG
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com