Rabbit Recombinant Monoclonal Fibrinogen gamma chain antibody. Carrier free. Suitable for ICC/IF, IP, WB, IHC-P and reacts with Human, Rat, Mouse samples.
pH: 7.2 - 7.4
Constituents: 100% PBS
ICC/IF | IP | Flow Cyt | WB | IHC-P | |
---|---|---|---|---|---|
Human | Tested | Tested | Not recommended | Tested | Tested |
Mouse | Not recommended | Tested | Not recommended | Tested | Not recommended |
Rat | Expected | Expected | Not recommended | Tested | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Together with fibrinogen alpha (FGA) and fibrinogen beta (FGB), polymerizes to form an insoluble fibrin matrix. Has a major function in hemostasis as one of the primary components of blood clots. In addition, functions during the early stages of wound repair to stabilize the lesion and guide cell migration during re-epithelialization. Was originally thought to be essential for platelet aggregation, based on in vitro studies using anticoagulated blood. However, subsequent studies have shown that it is not absolutely required for thrombus formation in vivo. Enhances expression of SELP in activated platelets via an ITGB3-dependent pathway. Maternal fibrinogen is essential for successful pregnancy. Fibrin deposition is also associated with infection, where it protects against IFNG-mediated hemorrhage. May also facilitate the antibacterial immune response via both innate and T-cell mediated pathways.
PRO2061, FGG, Fibrinogen gamma chain
Rabbit Recombinant Monoclonal Fibrinogen gamma chain antibody. Carrier free. Suitable for ICC/IF, IP, WB, IHC-P and reacts with Human, Rat, Mouse samples.
pH: 7.2 - 7.4
Constituents: 100% PBS
ab281944 is the carrier-free version of Anti-Fibrinogen gamma chain antibody [EP24539-127] ab281924.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
This data was developed using Anti-Fibrinogen gamma chain antibody [EP24539-127] ab281924, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 21480334).
Exposure time: Lanes 1-5: 10 seconds.
Lane 6: 37 seconds.
All lanes: Western blot - Anti-Fibrinogen gamma chain antibody [EP24539-127] (Anti-Fibrinogen gamma chain antibody [EP24539-127] ab281924) at 1/5000 dilution
Lane 1: Human plasma lysate at 10 µg
Lane 2: Human plasma lysate treated with PNGase F whole cell lysate at 10 µg
Lane 3: Mouse plasma lysate at 10 µg
Lane 4: Mouse plasma lysate treated with PNGase F whole cell lysate at 10 µg
Lane 5: Rat plasma lysate at 10 µg
Lane 6: Rat liver lysate at 10 µg
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/20000 dilution
Predicted band size: 52 kDa
Observed band size: 100 kDa, 50 kDa
This data was developed using Anti-Fibrinogen gamma chain antibody [EP24539-127] ab281924, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Low expression: A549/DU145 (PMID: 17949478).
All lanes: Western blot - Anti-Fibrinogen gamma chain antibody [EP24539-127] (Anti-Fibrinogen gamma chain antibody [EP24539-127] ab281924) at 1/1000 dilution
Lane 1: HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate at 10 µg
Lane 2: A549 (human lung carcinoma epithelial cell), whole cell lysate at 10 µg
Lane 3: DU145 (human prostate carcinoma epithelial cell), whole cell lysate at 10 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Predicted band size: 52 kDa
Observed band size: 50 kDa
Exposure time: 26s
This data was developed using Anti-Fibrinogen gamma chain antibody [EP24539-127] ab281924, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: Lanes 1: 1.
seconds Lane 2: 26 seconds.
All lanes: Western blot - Anti-Fibrinogen gamma chain antibody [EP24539-127] (Anti-Fibrinogen gamma chain antibody [EP24539-127] ab281924) at 1/1000 dilution
Lane 1: Human liver lysate at 10 µg
Lane 2: Mouse liver lysate at 10 µg
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/1000 dilution
Predicted band size: 52 kDa
Observed band size: 100 kDa, 50 kDa
This data was developed using Anti-Fibrinogen gamma chain antibody [EP24539-127] ab281924, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human liver tissue labelling Fibrinogen gamma chain with Anti-Fibrinogen gamma chain antibody [EP24539-127] ab281924 at 1/4000 (0.135 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human liver (PMID:24648543). The section was incubated with Anti-Fibrinogen gamma chain antibody [EP24539-127] ab281924 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
This data was developed using Anti-Fibrinogen gamma chain antibody [EP24539-127] ab281924, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labelling Fibrinogen gamma chain with Anti-Fibrinogen gamma chain antibody [EP24539-127] ab281924 at 1/4000 (0.135 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining in the blood plasma and stroma of human pancreas (PMID:16510577). The section was incubated with Anti-Fibrinogen gamma chain antibody [EP24539-127] ab281924 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
This data was developed using Anti-Fibrinogen gamma chain antibody [EP24539-127] ab281924, the same antibody clone in a different buffer formulation.
Fibrinogen gamma chain was immunoprecipitated from 0.35 mg HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate 10 ug with Anti-Fibrinogen gamma chain antibody [EP24539-127] ab281924 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Fibrinogen gamma chain antibody [EP24539-127] ab281924 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate 10 ug
Lane 2: Anti-Fibrinogen gamma chain antibody [EP24539-127] ab281924 IP in HepG2 whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Fibrinogen gamma chain antibody [EP24539-127] ab281924 in HepG2 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.
All lanes: Immunoprecipitation - Anti-Fibrinogen gamma chain antibody [EP24539-127] (Anti-Fibrinogen gamma chain antibody [EP24539-127] ab281924)
Predicted band size: 52 kDa
Observed band size: 50 kDa
This data was developed using Anti-Fibrinogen gamma chain antibody [EP24539-127] ab281924, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labelling Fibrinogen gamma chain with Anti-Fibrinogen gamma chain antibody [EP24539-127] ab281924 at 1/4000 (0.135 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human hepatocellular carcinoma (PMID:30863175). The section was incubated with Anti-Fibrinogen gamma chain antibody [EP24539-127] ab281924 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
This data was developed using Anti-Fibrinogen gamma chain antibody [EP24539-127] ab281924, the same antibody clone in a different buffer formulation.
Fibrinogen gamma chain was immunoprecipitated from 0.35 mg Mouse plasma lysate 10 ug with Anti-Fibrinogen gamma chain antibody [EP24539-127] ab281924 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Fibrinogen gamma chain antibody [EP24539-127] ab281924 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Mouse plasma lysate 10 ug
Lane 2: Anti-Fibrinogen gamma chain antibody [EP24539-127] ab281924 IP in Mouse plasma lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Fibrinogen gamma chain antibody [EP24539-127] ab281924 in mouse plasma lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.
All lanes: Immunoprecipitation - Anti-Fibrinogen gamma chain antibody [EP24539-127] (Anti-Fibrinogen gamma chain antibody [EP24539-127] ab281924)
Predicted band size: 52 kDa
Observed band size: 50 kDa
This data was developed using Anti-Fibrinogen gamma chain antibody [EP24539-127] ab281924, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized HepG2 cells labelling Fibrinogen gamma chain with Anti-Fibrinogen gamma chain antibody [EP24539-127] ab281924 at 1/100 (5.38 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green) Confocal image showing cytoplasmic staining in HepG2 cells is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
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