Rabbit Polyclonal Fibroblast activation protein, alpha antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 134 publications. Immunogen corresponding to Synthetic Peptide within Human FAP.
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- Frontiers in bioengineering and biotechnology 11:12918242023Development of a fibroblast activation protein-targeted PET/NIR dual-modality probe and its application in head and neck cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDanni Li,Xuran Li,Jiaojiao Li,Yanhong Wang,Fei Tan,Xiao LiPubMed 38026901
- Journal of nuclear cardiology : official publication of the American Society of Nuclear Cardiology 30:2712-27202023Fibroblast activation protein imaging in atrial fibrillation: a proof-of-concept study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLina Li,Jie Gao,Bi-Xi Chen,Xingpeng Liu,Liang Shi,Yanjiang Wang,Li Wang,Yidan Wang,Pixiong Su,Min-Fu Yang,Boqia XiePubMed 37626209
- Nature communications 14:42512023IL1R1 cancer-associated fibroblasts drive tumor development and immunosuppression in colorectal cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesE Koncina,M Nurmik,V I Pozdeev,C Gilson,M Tsenkova,R Begaj,S Stang,A Gaigneaux,C Weindorfer,F Rodriguez,M Schmoetten,E Klein,J Karta,V S Atanasova,K Grzyb,P Ullmann,R Halder,M Hengstschläger,J Graas,V Augendre,Y E Karapetyan,L Kerger,N Zuegel,A Skupin,S Haan,J Meiser,H Dolznig,E LetellierPubMed 37460545
- Advanced materials (Deerfield Beach, Fla.) 35:e23014932023Tenascin-C Activation of Lung Fibroblasts in a 3D Synthetic Lung Extracellular Matrix Mimic.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAritra Nath Kundu,Carey E Dougan,Samar Mahmoud,Alara Kilic,Alexi Panagiotou,Nathan R Richbourg,Ninette Irakoze,Shelly R PeytonPubMed 37227134
- Cancers 15:2023CTHRC1 Induces Pancreatic Stellate Cells (PSCs) into Myofibroblast-like Cancer-Associated Fibroblasts (myCAFs).Applications:Unspecified applicationReactive species:Unspecified reactive speciesMin Kyung Kang,Fen Jiang,Ye Ji Kim,Kyoungjin Ryu,Atsushi Masamune,Shin Hamada,Yun-Yong Park,Sang Seok KohPubMed 37444482
- Cell communication and signaling : CCS 21:1392023The Prognostic and therapeutic value and clinical implications of fibroblast activation protein-α as a novel biomarker in colorectal cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZahra Kalaei,Reyhaneh Manafi-Farid,Bentolhoda Rashidi,Fariba Karoon Kiani,Asieh Zarei,Mehrdad Fathi,Farhad Jadidi-NiaraghPubMed 37316886
- Heliyon 9:e149522023FGF21/FGFR1-β-KL cascade in cardiomyocytes modulates angiogenesis and inflammation under metabolic stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNamrita Kaur,Sanskruti Ravindra Gare,Andrea Ruiz-Velasco,Jessica M Miller,Riham R E Abouleisa,Qinghui Ou,Jiahan Shen,Handrean Soran,Tamer M A Mohamed,Wei LiuPubMed 37123894
- Journal of Cancer 14:464-4792023Different Expression and Clinical Implications of Cancer-Associated Fibroblast (CAF) Markers in Brain Metastases.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMd Rashedunnabi Akanda,Eun-Jung Ahn,Yeong Jin Kim,S M Abdus Salam,Myung-Giun Noh,Sung Sun Kim,Tae-Young Jung,In-Young Kim,Chang-Hyun Kim,Kyung-Hwa Lee,Kyung-Sub MoonPubMed 36860926
- American journal of physiology. Lung cellular and molecular physiology 324:L211-L2272023Indocyanine-enhanced mouse model of bleomycin-induced lung fibrosis with hallmarks of progressive emphysema.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAndrea Grandi,Erica Ferrini,Laura Mecozzi,Roberta Ciccimarra,Matteo Zoboli,Ludovica Leo,Zahra Khalajzeyqami,Alex Kleinjan,Clemens W G M Löwik,Gaetano Donofrio,Gino Villetti,Franco Fabio StellariPubMed 36625471
- The FEBS journal 290:2604-26152023A novel tdTomato transgenic mouse model to visualize FAP-positive cancer-associated fibroblasts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFeng Wei,Tomoyuki Uchihara,Atsuko Yonemura,Noriko Yasuda-Yoshihara,Tadahito Yasuda,Takashi Semba,Masahiro Fukuda,Takahiko Akiyama,Fumimasa Kitamura,Luke Bu,Xichen Hu,Lingfeng Fu,Jun Zhang,Ryusho Kariya,Juntaro Yamasaki,Kazuki Aihara,Kohei Yamashita,Osamu Nagano,Seiji Okada,Hideo Baba,Takatsugu IshimotoPubMed 36565059
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human FAP. The exact immunogen used to generate this antibody is proprietary information. Database link Q12884
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Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/1000 | Notes Please see WB protocol details in the image legend. |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 - 1/100 | Notes Antigen retrieval: Microwave method - put the slice into 10 mmol/L citrate buffer (pH 6.0), microwave high temperature for 5 minutes, and then medium temperature for 15 minutes. Primary antibody incubation: 1 hour at 37°C Secondary antibody: Poly-HRP-Anti Mouse/Rabbit IgG, 50 μL for 30 minutes. DAB reagent. |
Associated Products
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Target data
Function
Cell surface glycoprotein serine protease that participates in extracellular matrix degradation and involved in many cellular processes including tissue remodeling, fibrosis, wound healing, inflammation and tumor growth. Both plasma membrane and soluble forms exhibit post-proline cleaving endopeptidase activity, with a marked preference for Ala/Ser-Gly-Pro-Ser/Asn/Ala consensus sequences, on substrate such as alpha-2-antiplasmin SERPINF2 and SPRY2 (PubMed:14751930, PubMed:16223769, PubMed:16480718, PubMed:16410248, PubMed:17381073, PubMed:18095711, PubMed:21288888, PubMed:24371721). Degrade also gelatin, heat-denatured type I collagen, but not native collagen type I and IV, vitronectin, tenascin, laminin, fibronectin, fibrin or casein (PubMed:9065413, PubMed:2172980, PubMed:7923219, PubMed:10347120, PubMed:10455171, PubMed:12376466, PubMed:16223769, PubMed:16651416, PubMed:18095711). Also has dipeptidyl peptidase activity, exhibiting the ability to hydrolyze the prolyl bond two residues from the N-terminus of synthetic dipeptide substrates provided that the penultimate residue is proline, with a preference for Ala-Pro, Ile-Pro, Gly-Pro, Arg-Pro and Pro-Pro (PubMed:10347120, PubMed:10593948, PubMed:16175601, PubMed:16223769, PubMed:16651416, PubMed:16410248, PubMed:17381073, PubMed:21314817, PubMed:24371721, PubMed:24717288). Natural neuropeptide hormones for dipeptidyl peptidase are the neuropeptide Y (NPY), peptide YY (PYY), substance P (TAC1) and brain natriuretic peptide 32 (NPPB) (PubMed:21314817). The plasma membrane form, in association with either DPP4, PLAUR or integrins, is involved in the pericellular proteolysis of the extracellular matrix (ECM), and hence promotes cell adhesion, migration and invasion through the ECM. Plays a role in tissue remodeling during development and wound healing. Participates in the cell invasiveness towards the ECM in malignant melanoma cancers. Enhances tumor growth progression by increasing angiogenesis, collagen fiber degradation and apoptosis and by reducing antitumor response of the immune system. Promotes glioma cell invasion through the brain parenchyma by degrading the proteoglycan brevican. Acts as a tumor suppressor in melanocytic cells through regulation of cell proliferation and survival in a serine protease activity-independent manner.
Alternative names
Prolyl endopeptidase FAP, 170 kDa melanoma membrane-bound gelatinase, Dipeptidyl peptidase FAP, Fibroblast activation protein alpha, Gelatine degradation protease FAP, Integral membrane serine protease, Post-proline cleaving enzyme, Serine integral membrane protease, Surface-expressed protease, FAPalpha, SIMP, Seprase, FAP
Recommended products
Rabbit Polyclonal Fibroblast activation protein, alpha antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 134 publications. Immunogen corresponding to Synthetic Peptide within Human FAP.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human FAP. The exact immunogen used to generate this antibody is proprietary information. Database link Q12884
- Purification technique
Affinity purification Immunogen
- Specificity
ab53066 detects endogenous levels of total Fibroblast activation protein, alpha. Please note the immunogen used to produce this product has 92% homology with Mouse DPPIV/CD26.
- Concentration
- Purification notes
ab53066 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Storage information
Stable for 12 months at -20°C
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody (ab53066)
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue sections labeling Fibroblast activation protein, alpha with ab53066 (without peptide-right and with peptide- left).
Western blot - Anti-Fibroblast activation protein, alpha antibody (ab53066)
Blocking buffer: 5% (w/v) non-fat dry milk in TBST.
Primary antibody dilution buffer: 5%（w/v）non-fat dried milk，0.1%（v/v）, Tween-20 in TBST.
Secondary antibody dilution buffer: 5%（w/v）non-fat dried milk，0.1%（v/v），Tween-20 in TBST.
12% SDS gel. Nitrocellulose membrane.
Blocking: Room temperature for 2 hours or overnight at 4°C. Then wash 3x for 5 minutes with 0.05% blocking buffer.
Primary antibody incubation: diluted in TBST at 1/500. Incubate overnight with 4 degrees shaking. Then, in 0.05% TBST, wash membrane 3-4 times for 10min.
Secondary antibody incubation: diluted in TBST at 1/2000. Incubate 37°C for 1 hour. Then, in 0.05% TBST, wash membrane 3-4 times for 10min.
ECL development.
All lanes: Western blot - Anti-Fibroblast activation protein, alpha antibody (ab53066) at 1/500 dilution
Lane 1: LOVO cell extract
Lane 2: LOVO cell extract at 40 µg
Predicted band size: 88 kDa
Observed band size: 90 kDa
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