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Anti-Fibroblast activation protein, alpha antibody [EPR20021] (ab207178) is a rabbit monoclonal antibody detecting Fibroblast activation protein, alpha in Western Blot, IHC-P. Suitable for Human.



- Biophysical QC for unrivalled batch-batch consistency

- Over 80 publications



Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [EPR20021] (AB207178), expandable thumbnail
  • Western blot - Anti-Fibroblast activation protein, alpha antibody [EPR20021] (AB207178), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [EPR20021] (AB207178), expandable thumbnail
  • Western blot - Anti-Fibroblast activation protein, alpha antibody [EPR20021] (AB207178), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [EPR20021] (AB207178), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1/250
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

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Target data

Function

The protein expressed by the FAP gene is a cell surface glycoprotein serine protease involved in the degradation of the extracellular matrix (ECM) and various cellular processes such as tissue remodeling, fibrosis, wound healing, inflammation, and tumor growth. It exists in both plasma membrane-bound and soluble forms, exhibiting post-proline cleaving endopeptidase activity, specifically targeting Ala/Ser-Gly-Pro-Ser/Asn/Ala sequences in substrates like alpha-2-antiplasmin SERPINF2 and SPRY2. FAP protein degrades gelatin and heat-denatured type I collagen but not native type I and IV collagens, nor vitronectin, tenascin, laminin, fibronectin, fibrin, or casein. It also has dipeptidyl peptidase activity, hydrolyzing prolyl bonds when the penultimate residue is proline, with a preference for sequences such as Ala-Pro and Gly-Pro. It acts on natural neuropeptides like neuropeptide Y, peptide YY, substance P, and brain natriuretic peptide 32. In its membrane form, in association with DPP4, PLAUR, or integrins, FAP is involved in pericellular ECM proteolysis, promoting cell adhesion, migration, invasion, and plays roles in development, wound healing, and cell invasiveness in malignant melanoma. It supports tumor progression by facilitating angiogenesis, collagen degradation, apoptosis, and reducing immune response. Additionally, it promotes glioma cell invasion by degrading brevican and functions as a tumor suppressor in melanocytic cells by regulating cell proliferation and survival independently of its serine protease activity. This supplementary information is collated from multiple sources and compiled automatically.

Alternative names

Prolyl endopeptidase FAP, 170 kDa melanoma membrane-bound gelatinase, Dipeptidyl peptidase FAP, Fibroblast activation protein alpha, Gelatine degradation protease FAP, Integral membrane serine protease, Post-proline cleaving enzyme, Serine integral membrane protease, Surface-expressed protease, FAPalpha, SIMP, Seprase, FAP

Recommended products

Anti-Fibroblast activation protein, alpha antibody [EPR20021] (ab207178) is a rabbit monoclonal antibody detecting Fibroblast activation protein, alpha in Western Blot, IHC-P. Suitable for Human.



- Biophysical QC for unrivalled batch-batch consistency

- Over 80 publications


Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR20021
Purification technique
Affinity purification Protein A
Specificity

Except for the 95kDa form, 100-120kDa or even higher molecular weights were also observed due to glycosylation (PMID: 24551161, PMID: 28452380).

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Anti-Fibroblast activation protein, alpha antibody [EPR20021] (ab207178) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in IHC-P, WB in human samples.

Anti-Fibroblast activation protein, alpha antibody [EPR20021] (ab207178) has been cited over 85 times in peer reviewed journals and is trusted by the scientific community.

Abcams high quality manufacturing and validation processes ensure Anti-Fibroblast activation protein, alpha antibody [EPR20021] (ab207178) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.

Anti-Fibroblast activation protein, alpha antibody [EPR20021] (ab207178) has 16 independent reviews from customers.

Anti-Fibroblast activation protein, alpha antibody [EPR20021] (ab207178) specifically detects Fibroblast activation protein, alpha (UniProt ID: Q12884; Molecular weight: 88kDa) and is sold in 100 uL and 1 mL selling sizes.

Conjugation-ready, carrier free format available for antibody clone EPR20021 - Anti-Fibroblast activation protein, alpha antibody [EPR20021] - BSA and Azide free ab271976.

Fibroblast activation protein-alpha (FAP) is a serine protease predominantly expressed by cancer-associated fibroblasts (CAFs) in the tumor microenvironment. It promotes tumor growth, invasion, and immunosuppression by remodeling the extracellular matrix and influencing intracellular signaling pathways.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

12 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [EPR20021] (ab207178), expandable thumbnail
    Courtesy of an anonymous customer review

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [EPR20021] (ab207178)

    Formalin-fixed, paraffin-embedded human colon cancer tissue labeling Fibroblast activation protein, alpha using ab207178 at 1/100 dilution in immunohistochemical analysis. Heat mediated antigen retieval using Citrate, pH 6.0.

  • Western blot - Anti-Fibroblast activation protein, alpha antibody [EPR20021] (ab207178), expandable thumbnail

    Western blot - Anti-Fibroblast activation protein, alpha antibody [EPR20021] (ab207178)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The molecular weight is consistent with the literature.

    Negative control: Human fetal liver (PMID: 10593948, PMID: 9139873).

    All lanes: Western blot - Anti-Fibroblast activation protein, alpha antibody [EPR20021] (ab207178) at 1/1000 dilution

    Lane 1: WI-38 (Human fetal lung fibroblast cell line) whole cell lysate at 10 µg

    Lane 2: U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate at 10 µg

    Lane 3: Human fetal liver lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 88 kDa

    Observed band size: 95 kDa

    Exposure time: 3min

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [EPR20021] (ab207178), expandable thumbnail
    This image is of an Abreciew submitted byCarl Hobbs.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [EPR20021] (ab207178)

    Immunohistochemical analysis of formaldehyde fixed paraffin embedded human Ovarian carcinoma tissue sectioins labeling Fibroblast activation protein with ab207178 at 1/500. Biotin conjugated goat anti-rabbit IgG at 1/300 was used as the secondary antibody. Antigen retrieval was heat mediated using citric acid.

  • Western blot - Anti-Fibroblast activation protein, alpha antibody [EPR20021] (ab207178), expandable thumbnail

    Western blot - Anti-Fibroblast activation protein, alpha antibody [EPR20021] (ab207178)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 1 minute; Lane 2: 3 minutes.

    The molecular weight is consistent with the literature (PMID: 10593948).

    All lanes: Western blot - Anti-Fibroblast activation protein, alpha antibody [EPR20021] (ab207178) at 1/1000 dilution

    Lane 1: HFF-1 (Human forskin fibroblast cell line) whole cell lysate at 10 µg

    Lane 2: IMR-90 (Human lung fibroblast cell line) whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 88 kDa

    Observed band size: 95 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [EPR20021] (ab207178), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [EPR20021] (ab207178)

    Immunohistochemical analysis of paraffin-embedded human pancreatic cancer tissue labeling Fibroblast activation protein, alpha with ab207178 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Cell surface staining on stromal cells of human pancreas cancer is observed [PMID:2371645].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-Fibroblast activation protein, alpha antibody [EPR20021] (ab207178), expandable thumbnail

    Western blot - Anti-Fibroblast activation protein, alpha antibody [EPR20021] (ab207178)

    Different batches of ab207178 were tested on IMR-90 (Human lung fibroblast cell line) lysate at 1.0 µg/ml. 15 µg of lysate was loaded in each lane. Bands observed at 95 kDa.

    All lanes: Western blot - Anti-Fibroblast activation protein, alpha antibody [EPR20021] (ab207178)

    Predicted band size: 88 kDa

    Observed band size: 95 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [EPR20021] (ab207178), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [EPR20021] (ab207178)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling Fibroblast activation protein, alpha with ab207178 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Cell surface staining on stromal cells of human breast cancer is observed [PMID:18628473].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [EPR20021] (ab207178), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [EPR20021] (ab207178)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling Fibroblast activation protein, alpha with ab207178 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Negative control: no staining on human pancreas [PMID:22371645].

    Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [EPR20021] (ab207178), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [EPR20021] (ab207178)

    Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Fibroblast activation protein, alpha with ab207178 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Negative control: no staining on human liver [PMID: 9139873].

    Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [EPR20021] (ab207178), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [EPR20021] (ab207178)

    Immunohistochemical analysis of paraffin-embedded Human stomach cancer tissue labeling Fibroblast activation protein, alpha with ab207178 at 1/1000 (0.718 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on stromal fibroblast cells of human stomach cancer. The section was incubated with ab207178 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection)
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [EPR20021] (ab207178), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [EPR20021] (ab207178)

    Immunohistochemical analysis of paraffin-embedded Human sciatic nerve tissue labeling Fibroblast activation protein, alpha with ab207178 at 1/1000 (0.718 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: no staining on human sciatic nerve. The section was incubated with ab207178 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection)
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [EPR20021] (ab207178), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [EPR20021] (ab207178)

    Immunohistochemical analysis of formalin fixed paraffin embedded human tonsil labelling Fibroblast activation protein, alpha with ab207178 at a concentration of 3 µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with an OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was conducted for 32 mins at 100°C with ULTRA cell conditioning solution (CC1, pH 8.5). ab207178 anti- Fibroblast activation protein, alpha [EPR20021] was incubated at 37°C for 16 mins. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

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