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anti-Fibroblast activation protein, alpha antibody [EPR20021] ab271976 is a carrier free rabbit monoclonal antibody that is used in Fibroblast activation protein, alpha western blotting and IHC. Suitable for human samples.

- PBS only, conjugation-ready, removing anything extra from your antibodies, greater flexibility in assay design
- Antibody clone EPR20021 is the most widely used clone for Fibroblast activation protein, alpha on the market
- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray validation


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [EPR20021] - BSA and Azide free (AB271976), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [EPR20021] - BSA and Azide free (AB271976), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [EPR20021] - BSA and Azide free (AB271976), expandable thumbnail
  • Western blot - Anti-Fibroblast activation protein, alpha antibody [EPR20021] - BSA and Azide free (AB271976), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [EPR20021] - BSA and Azide free (AB271976), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Associated Products

Select an associated product type

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Target data

Function

Cell surface glycoprotein serine protease that participates in extracellular matrix degradation and involved in many cellular processes including tissue remodeling, fibrosis, wound healing, inflammation and tumor growth. Both plasma membrane and soluble forms exhibit post-proline cleaving endopeptidase activity, with a marked preference for Ala/Ser-Gly-Pro-Ser/Asn/Ala consensus sequences, on substrate such as alpha-2-antiplasmin SERPINF2 and SPRY2 (PubMed:14751930, PubMed:16223769, PubMed:16410248, PubMed:16480718, PubMed:17381073, PubMed:18095711, PubMed:21288888, PubMed:24371721). Degrade also gelatin, heat-denatured type I collagen, but not native collagen type I and IV, vitronectin, tenascin, laminin, fibronectin, fibrin or casein (PubMed:10347120, PubMed:10455171, PubMed:12376466, PubMed:16223769, PubMed:16651416, PubMed:18095711, PubMed:2172980, PubMed:7923219, PubMed:9065413). Also has dipeptidyl peptidase activity, exhibiting the ability to hydrolyze the prolyl bond two residues from the N-terminus of synthetic dipeptide substrates provided that the penultimate residue is proline, with a preference for Ala-Pro, Ile-Pro, Gly-Pro, Arg-Pro and Pro-Pro (PubMed:10347120, PubMed:10593948, PubMed:16175601, PubMed:16223769, PubMed:16410248, PubMed:16651416, PubMed:17381073, PubMed:21314817, PubMed:24371721, PubMed:24717288). Natural neuropeptide hormones for dipeptidyl peptidase are the neuropeptide Y (NPY), peptide YY (PYY), substance P (TAC1) and brain natriuretic peptide 32 (NPPB) (PubMed:21314817). The plasma membrane form, in association with either DPP4, PLAUR or integrins, is involved in the pericellular proteolysis of the extracellular matrix (ECM), and hence promotes cell adhesion, migration and invasion through the ECM. Plays a role in tissue remodeling during development and wound healing. Participates in the cell invasiveness towards the ECM in malignant melanoma cancers. Enhances tumor growth progression by increasing angiogenesis, collagen fiber degradation and apoptosis and by reducing antitumor response of the immune system. Promotes glioma cell invasion through the brain parenchyma by degrading the proteoglycan brevican. Acts as a tumor suppressor in melanocytic cells through regulation of cell proliferation and survival in a serine protease activity-independent manner.

Alternative names

Prolyl endopeptidase FAP, 170 kDa melanoma membrane-bound gelatinase, Dipeptidyl peptidase FAP, Fibroblast activation protein alpha, Gelatine degradation protease FAP, Integral membrane serine protease, Post-proline cleaving enzyme, Serine integral membrane protease, Surface-expressed protease, FAPalpha, SIMP, Seprase, FAP

Recommended products

anti-Fibroblast activation protein, alpha antibody [EPR20021] ab271976 is a carrier free rabbit monoclonal antibody that is used in Fibroblast activation protein, alpha western blotting and IHC. Suitable for human samples.

- PBS only, conjugation-ready, removing anything extra from your antibodies, greater flexibility in assay design
- Antibody clone EPR20021 is the most widely used clone for Fibroblast activation protein, alpha on the market
- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray validation

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR20021
Purification technique
Affinity purification Protein A
Specificity

Except for the 95kDa form, 100-120kDa or even higher molecular weights were also observed due to glycosylation (PMID: 24551161, PMID: 28452380).

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab271976 is the carrier-free version of Anti-Fibroblast activation protein, alpha antibody [EPR20021] ab207178.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

11 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [EPR20021] - BSA and Azide free (ab271976), expandable thumbnail
    Courtesy of an anonymous customer review

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [EPR20021] - BSA and Azide free (ab271976)

    Formalin-fixed, paraffin-embedded human colon cancer tissue labeling Fibroblast activation protein, alpha using Anti-Fibroblast activation protein, alpha antibody [EPR20021] ab207178 at 1/100 dilution in immunohistochemical analysis. Heat mediated antigen retieval using Citrate, pH 6.0.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Fibroblast activation protein, alpha antibody [EPR20021] ab207178).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [EPR20021] - BSA and Azide free (ab271976), expandable thumbnail
    This image is of an Abreciew submitted byCarl Hobbs.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [EPR20021] - BSA and Azide free (ab271976)

    Immunohistochemical analysis of formaldehyde fixed paraffin embedded human Ovarian carcinoma tissue sectioins labeling Fibroblast activation protein with Anti-Fibroblast activation protein, alpha antibody [EPR20021] ab207178 at 1/500. Biotin conjugated goat anti-rabbit IgG at 1/300 was used as the secondary antibody. Antigen retrieval was heat mediated using citric acid. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Fibroblast activation protein, alpha antibody [EPR20021] ab207178).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [EPR20021] - BSA and Azide free (ab271976), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [EPR20021] - BSA and Azide free (ab271976)

    Immunohistochemical analysis of paraffin-embedded human pancreatic cancer tissue labeling Fibroblast activation protein, alpha with Anti-Fibroblast activation protein, alpha antibody [EPR20021] ab207178 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Cell surface staining on stromal cells of human pancreas cancer is observed [PMID:2371645].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Fibroblast activation protein, alpha antibody [EPR20021] ab207178).

  • Western blot - Anti-Fibroblast activation protein, alpha antibody [EPR20021] - BSA and Azide free (ab271976), expandable thumbnail

    Western blot - Anti-Fibroblast activation protein, alpha antibody [EPR20021] - BSA and Azide free (ab271976)

    This data was developed using Anti-Fibroblast activation protein, alpha antibody [EPR20021] ab207178, the same antibody clone in a different buffer formulation. Different batches of Anti-Fibroblast activation protein, alpha antibody [EPR20021] ab207178 were tested on IMR-90 (Human lung fibroblast cell line) lysate at 1.0 µg/ml. 15 µg of lysate was loaded in each lane. Bands observed at 95 kDa.

    All lanes: Western blot - Anti-Fibroblast activation protein, alpha antibody [EPR20021] (Anti-Fibroblast activation protein, alpha antibody [EPR20021] ab207178)

    Predicted band size: 88 kDa

    Observed band size: 95 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [EPR20021] - BSA and Azide free (ab271976), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [EPR20021] - BSA and Azide free (ab271976)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling Fibroblast activation protein, alpha with Anti-Fibroblast activation protein, alpha antibody [EPR20021] ab207178 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Cell surface staining on stromal cells of human breast cancer is observed [PMID:18628473].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Fibroblast activation protein, alpha antibody [EPR20021] ab207178).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [EPR20021] - BSA and Azide free (ab271976), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [EPR20021] - BSA and Azide free (ab271976)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling Fibroblast activation protein, alpha with Anti-Fibroblast activation protein, alpha antibody [EPR20021] ab207178 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Negative control: no staining on human pancreas [PMID:22371645].

    Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Fibroblast activation protein, alpha antibody [EPR20021] ab207178).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [EPR20021] - BSA and Azide free (ab271976), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [EPR20021] - BSA and Azide free (ab271976)

    Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Fibroblast activation protein, alpha with Anti-Fibroblast activation protein, alpha antibody [EPR20021] ab207178 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Negative control: no staining on human liver [PMID: 9139873].

    Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Fibroblast activation protein, alpha antibody [EPR20021] ab207178).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [EPR20021] - BSA and Azide free (ab271976), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [EPR20021] - BSA and Azide free (ab271976)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Fibroblast activation protein, alpha antibody [EPR20021] ab207178).
    Immunohistochemical analysis of paraffin-embedded Human stomach cancer tissue labeling Fibroblast activation protein, alpha with Anti-Fibroblast activation protein, alpha antibody [EPR20021] ab207178 at 1/1000 (0.718 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on stromal fibroblast cells of human stomach cancer. The section was incubated with Anti-Fibroblast activation protein, alpha antibody [EPR20021] ab207178 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection)
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [EPR20021] - BSA and Azide free (ab271976), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [EPR20021] - BSA and Azide free (ab271976)

    Immunohistochemical analysis of formalin fixed paraffin embedded human colon carcinoma labelling fibroblast activation protein alpha with ab271976 at a concentration of 5µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with an OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was conducted for 32min with ULTRA cell conditioning solution (CC1 pH8.5). ab271976 anti fibroblast activation protein alpha antibody was incubated at 37oC for 16min. Sections were counterstained is with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [EPR20021] - BSA and Azide free (ab271976), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [EPR20021] - BSA and Azide free (ab271976)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Fibroblast activation protein, alpha antibody [EPR20021] ab207178).
    Immunohistochemical analysis of paraffin-embedded Human sciatic nerve tissue labeling Fibroblast activation protein, alpha with Anti-Fibroblast activation protein, alpha antibody [EPR20021] ab207178 at 1/1000 (0.718 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: no staining on human sciatic nerve. The section was incubated with Anti-Fibroblast activation protein, alpha antibody [EPR20021] ab207178 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection)
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [EPR20021] - BSA and Azide free (ab271976), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [EPR20021] - BSA and Azide free (ab271976)

    This data was developed using the same antibody clone in a different buffer formulation (Anti-Fibroblast activation protein, alpha antibody [EPR20021] ab207178).

    Immunohistochemical analysis of formalin fixed paraffin embedded human tonsil labelling Fibroblast activation protein, alpha with Anti-Fibroblast activation protein, alpha antibody [EPR20021] ab207178 at a concentration of 3 µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with an OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was conducted for 32 mins at 100°C with ULTRA cell conditioning solution (CC1, pH 8.5). Anti-Fibroblast activation protein, alpha antibody [EPR20021] ab207178 anti- Fibroblast activation protein, alpha [EPR20021] was incubated at 37°C for 16 mins. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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