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AB317556

Anti-Fibroblast activation protein, alpha antibody [EPR27358-9] - BSA and Azide free

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Rabbit Recombinant Monoclonal Fibroblast activation protein, alpha antibody. Carrier free. Suitable for Flow Cyt and reacts with Transfected cell line, Human, Mouse samples.

View Alternative Names

Prolyl endopeptidase FAP, Dipeptidyl peptidase FAP, Fibroblast activation protein alpha, Gelatine degradation protease FAP, Integral membrane serine protease, Post-proline cleaving enzyme, Serine integral membrane protease, Surface-expressed protease, FAPalpha, SIMP, Seprase, Fap

3 Images
Flow Cytometry - Anti-Fibroblast activation protein, alpha antibody [EPR27358-9] - BSA and Azide free (AB317556)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-Fibroblast activation protein, alpha antibody [EPR27358-9] - BSA and Azide free (AB317556)

This data was developed using ab317555, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of Isotype (Left) / 293T cells transfected with a Fibroblast activation protein, alpha expression vector containing a myc-His-tag® (Middle) / 293T cells transfected with an empty expression vector containing a myc-His-tag® (Right) cells labelling Fibroblast activation protein, alpha with ab317555 at 1/5000 dilution (0.01 ug)/Middle and Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Flow Cytometry - Anti-Fibroblast activation protein, alpha antibody [EPR27358-9] - BSA and Azide free (AB317556)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-Fibroblast activation protein, alpha antibody [EPR27358-9] - BSA and Azide free (AB317556)

This data was developed using ab317555, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of Jurkat(human T cell leukemia T lymphocyte from peripheral blood, Left) / WI-38(human fetal lung fibroblast, Right) cells labelling Fibroblast activation protein, alpha with ab317555 at 1/50 dilution (1 ug)/Red compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cell.
Negative control : Jurkat.

Flow Cytometry - Anti-Fibroblast activation protein, alpha antibody [EPR27358-9] - BSA and Azide free (AB317556)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-Fibroblast activation protein, alpha antibody [EPR27358-9] - BSA and Azide free (AB317556)

This data was developed using ab317555, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of MEF(mouse embryo fibroblast, Top left) / C2C12(mouse myoblast, Top right) / RAW 264.7(mouse Abelson murine leukemia virus-induced tumor macrophage, Bottom left) / EL4(mouse lymphoma T lymphocyte, Bottom right) cells labelling Fibroblast activation protein, alpha with ab317555 at 1/50 dilution (1 ug)/Red compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cell.
Negative control : RAW 264.7(PMID : 512331) and EL4(PMID : 16322266).

  • Unconjugated

    Anti-Fibroblast activation protein, alpha antibody [EPR27358-9]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR27358-9

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Assay Kits

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Primary Antibodies

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Secondary Antibodies

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Primary Antibodies

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Primary Antibodies

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Reactivity data

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Product details

ab317556 is the carrirer-free version of ab317555.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cell surface glycoprotein serine protease that participates in extracellular matrix degradation and involved in many cellular processes including tissue remodeling, fibrosis, wound healing, inflammation and tumor growth. Both plasma membrane and soluble forms exhibit post-proline cleaving endopeptidase activity, with a marked preference for Ala/Ser-Gly-Pro-Ser/Asn/Ala consensus sequences, on substrate such as alpha-2-antiplasmin SERPINF2 and SPRY2. Degrade also gelatin, heat-denatured type I collagen, but not native collagen type I and IV, vibronectin, tenascin, laminin, fibronectin, fibrin or casein. Also has dipeptidyl peptidase activity, exhibiting the ability to hydrolyze the prolyl bond two residues from the N-terminus of synthetic dipeptide substrates provided that the penultimate residue is proline, with a preference for Ala-Pro, Ile-Pro, Gly-Pro, Arg-Pro and Pro-Pro. Natural neuropeptide hormones for dipeptidyl peptidase are the neuropeptide Y (NPY), peptide YY (PYY), substance P (TAC1) and brain natriuretic peptide 32 (NPPB). The plasma membrane form, in association with either DPP4, PLAUR or integrins, is involved in the pericellular proteolysis of the extracellular matrix (ECM), and hence promotes cell adhesion, migration and invasion through the ECM. Plays a role in tissue remodeling during development and wound healing. Participates in the cell invasiveness towards the ECM in malignant melanoma cancers. Enhances tumor growth progression by increasing angiogenesis, collagen fiber degradation and apoptosis and by reducing antitumor response of the immune system. Promotes glioma cell invasion through the brain parenchyma by degrading the proteoglycan brevican. Acts as a tumor suppressor in melanocytic cells through regulation of cell proliferation and survival in a serine protease activity-independent manner.
See full target information Fap
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com