Anti-Fibroblast activation protein, alpha antibody [SP325]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [SP325] (AB227703)

Formalin-fixed paraffin-embedded human ovarian adenocarcinoma tissue stained for Fibroblast activation protein alpha using ab227703 at 1/100 dilution in immunohistochemical analysis.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [SP325] (AB227703)

Formalin-fixed paraffin-embedded human melanoma tissue stained for Fibroblast activation protein alpha using ab227703 at 1/100 dilution in immunohistochemical analysis.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [SP325] (AB227703)

Formalin-fixed paraffin-embedded human colon adenocarcinoma tissue stained for Fibroblast activation protein alpha using ab227703 at 1/100 dilution in immunohistochemical analysis.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [SP325] (AB227703)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon carcinoma tissue sections labeling Fibroblast activation protein, alpha with ab227703 at 1/100 dilution (1.76 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the stroma in the human colon carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab227703 for 10 mins at room temperature.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [SP325] (AB227703)

Formalin-fixed paraffin-embedded human skin squamous cell carcinoma tissue stained for Fibroblast activation protein alpha using ab227703 at 1/100 dilution in immunohistochemical analysis.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [SP325] (AB227703)

Formalin-fixed paraffin-embedded human pancreatic adenocarcinoma tissue stained for Fibroblast activation protein alpha using ab227703 at 1/100 dilution in immunohistochemical analysis.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [SP325] (AB227703)

Formalin-fixed paraffin-embedded human lung squamous cell carcinoma tissue stained for Fibroblast activation protein alpha using ab227703 at 1/100 dilution in immunohistochemical analysis.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [SP325] (AB227703)

Formalin-fixed paraffin-embedded human cervical squamous cell carcinoma tissue stained for Fibroblast activation protein alpha using ab227703 at 1/100 dilution in immunohistochemical analysis.

• WB

Lab

Western blot - Anti-Fibroblast activation protein, alpha antibody [SP325] (AB227703)

Western blot : Rabbit Monoclonal[SP325] to Fibroblast activation protein, alpha ab227703 staining at 0.176 µg/mL, shown in green; Mouse anti GAPDH ab8245 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 90-100 kDa in Wild-type U-87 MG cell lysates with no signal observed at this size in FAP knockout U-87 MG cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-Fibroblast activation protein, alpha antibody [SP325] (ab227703) at 0.176 µg/mL

Lane 1:

Wild-type U-87 MG at 20 µg

Lane 2:

Western blot - Human FAP knockout U-87 MG cell line (ab306819) at 20 µg

Lane 3:

WI-38 at 20 µg

Lane 4:

A549 at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 88 kDa

Observed band size: 90-100 kDa,36 kDa

false

• WB

Lab

Western blot - Anti-Fibroblast activation protein, alpha antibody [SP325] (AB227703)

Blocking/Diluting buffer : 5% NFDM /TBST

Lane 1:

Western blot - Anti-Fibroblast activation protein, alpha antibody [SP325] (ab227703) at 0.176 µg/mL

Lane 2:

Western blot - Anti-Fibroblast activation protein, alpha antibody [SP325] (ab227703)

Lane 1:

HFF-1 (Human Skin fibroblast) cell lysates at 20 µg

Lane 2:

HFF-1 (Human Skin fibroblast) at 20 µg

Secondary

Lane 1:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 0.05 µg/mL

Lane 2:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 0.05 µg

Predicted band size: 88 kDa

Observed band size: 95 kDa

false