Anti-Fibroblast activation protein, alpha antibody [SP325] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [SP325] - BSA and Azide free (AB240989)

Formalin-fixed, paraffin-embedded human ovarian adenocarcinoma tissue stained for Fibroblast activation protein, alpha using ab227703 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227703)

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [SP325] - BSA and Azide free (AB240989)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon carcinoma tissue sections labeling Fibroblast activation protein, alpha with ab227703 at 1/100 dilution (1.76 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the stroma in the human colon carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab227703 for 10 mins at room temperature.

This image was generated using ab227703, the same clone, but with a different buffer formulation.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [SP325] - BSA and Azide free (AB240989)

Formalin-fixed, paraffin-embedded human skin squamous cell carcinoma tissue stained for Fibroblast activation protein, alpha using ab227703 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227703)

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [SP325] - BSA and Azide free (AB240989)

Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma tissue stained for Fibroblast activation protein, alpha using ab227703 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227703)

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [SP325] - BSA and Azide free (AB240989)

Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for Fibroblast activation protein, alpha using ab227703 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227703)

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [SP325] - BSA and Azide free (AB240989)

Formalin-fixed, paraffin-embedded human cervical squamous cell carcinoma tissue stained for Fibroblast activation protein, alpha using ab227703 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227703)

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [SP325] - BSA and Azide free (AB240989)

Formalin-fixed, paraffin-embedded human melanoma tissue stained for Fibroblast activation protein, alpha using ab227703 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227703)

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [SP325] - BSA and Azide free (AB240989)

Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for Fibroblast activation protein, alpha using ab227703 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227703)

• WB

Lab

Western blot - Anti-Fibroblast activation protein, alpha antibody [SP325] - BSA and Azide free (AB240989)

This data was developed using ab227703, the same antibody clone in a different buffer formulation.

Western blot : Rabbit Monoclonal[SP325] to Fibroblast activation protein, alpha ab227703 staining at 0.176 µg/mL, shown in green; Mouse anti GAPDH ab8245 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 90-100 kDa in Wild-type U-87 MG cell lysates with no signal observed at this size in FAP knockout U-87 MG cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-Fibroblast activation protein, alpha antibody [SP325] (<a href='/en-us/products/primary-antibodies/fibroblast-activation-protein-alpha-antibody-sp325-ab227703'>ab227703</a>) at 0.176 µg/mL

Lane 1:

Wild-type U-87 MG at 20 µg

Lane 2:

Western blot - Human FAP knockout U-87 MG cell line (ab306819) at 20 µg

Lane 3:

WI-38 at 20 µg

Lane 4:

A549 at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 88 kDa

Observed band size: 90-100 kDa,36 kDa

false